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. 1991 Mar 1;274(Pt 2):615–617. doi: 10.1042/bj2740615

Relative rates of biosynthesis of collagen type I, type V and type VI in calf cornea.

P Kern 1, M Menasche 1, L Robert 1
PMCID: PMC1150184  PMID: 2006924

Abstract

The biosynthesis of type I, type V and type VI collagens was studied by incubation of calf corneas in vitro with [3H]proline as a marker. Pepsin-solubilized collagen types were isolated by salt fractionation and quantified by SDS/PAGE. Expressed as proportions of the total hydroxyproline solubilized, corneal stroma comprised 75% type I, 8% type V and 17% type VI collagen. The rates of [3H]proline incorporation, linear up to 24 h for each collagen type, were highest for type VI collagen and lowest for type I collagen. From pulse-chase experiments, the calculated apparent half-lives for types I, V and VI collagens were 36 h, 10 h and 6 h respectively.

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Selected References

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  1. Asselot C., Labat-Robert J., Kern P. Heparin fragments regulate collagen phenotype and fibronectin synthesis in the skin of genetically diabetic mice. Biochem Pharmacol. 1989 Mar 15;38(6):895–899. doi: 10.1016/0006-2952(89)90277-3. [DOI] [PubMed] [Google Scholar]
  2. Bruns R. R. Beaded filaments and long-spacing fibrils: relation to type VI collagen. J Ultrastruct Res. 1984 Nov;89(2):136–145. doi: 10.1016/s0022-5320(84)80010-6. [DOI] [PubMed] [Google Scholar]
  3. Bruns R. R., Press W., Engvall E., Timpl R., Gross J. Type VI collagen in extracellular, 100-nm periodic filaments and fibrils: identification by immunoelectron microscopy. J Cell Biol. 1986 Aug;103(2):393–404. doi: 10.1083/jcb.103.2.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cintron C., Hong B. S. Heterogeneity of collagens in rabbit cornea: type VI collagen. Invest Ophthalmol Vis Sci. 1988 May;29(5):760–766. [PubMed] [Google Scholar]
  5. Davison P. F., Hong B. S., Cannon D. J. Quantitative analysis of the collagens in the bovine cornea. Exp Eye Res. 1979 Aug;29(2):97–107. doi: 10.1016/0014-4835(79)90075-7. [DOI] [PubMed] [Google Scholar]
  6. Kern P., Moczar M., Robert L. Biosynthesis of skin collagens in normal and diabetic mice. Biochem J. 1979 Aug 15;182(2):337–345. doi: 10.1042/bj1820337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kern P., Robert L., Courtois Y., Laurent M. Selective decrease of type I collagen synthesis in Fraser mice skin. Biochim Biophys Acta. 1985 Dec 18;826(4):174–179. doi: 10.1016/0167-4781(85)90003-x. [DOI] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Linsenmayer T. F., Bruns R. R., Mentzer A., Mayne R. Type VI collagen: immunohistochemical identification as a filamentous component of the extracellular matrix of the developing avian corneal stroma. Dev Biol. 1986 Dec;118(2):425–431. doi: 10.1016/0012-1606(86)90013-8. [DOI] [PubMed] [Google Scholar]
  10. Linsenmayer T. F., Fitch J. M., Birk D. E. Heterotypic collagen fibrils and stabilizing collagens. Controlling elements in corneal morphogenesis? Ann N Y Acad Sci. 1990;580:143–160. doi: 10.1111/j.1749-6632.1990.tb17926.x. [DOI] [PubMed] [Google Scholar]
  11. Linsenmayer T. F., Fitch J. M., Gross J., Mayne R. Are collagen fibrils in the developing avian cornea composed of two different collagen types? Evidence from monoclonal antibody studies. Ann N Y Acad Sci. 1985;460:232–245. doi: 10.1111/j.1749-6632.1985.tb51171.x. [DOI] [PubMed] [Google Scholar]
  12. Linsenmayer T. F., Mentzer A., Irwin M. H., Waldrep N. K., Mayne R. Avian type VI collagen. Monoclonal antibody production and immunohistochemical identification as a major connective tissue component of cornea and skeletal muscle. Exp Cell Res. 1986 Aug;165(2):518–529. doi: 10.1016/0014-4827(86)90604-x. [DOI] [PubMed] [Google Scholar]
  13. Menasche M., Junqua S., Payrau P., Robert L. Etude de la régulation de la biosynthèse des macromolécules de la matrice intercellulaire de la cornée. Pathol Biol (Paris) 1975 Nov;23(9):705–709. [PubMed] [Google Scholar]
  14. Newsome D. A., Gross J., Hassell J. R. Human corneal stroma contains three distinct collagens. Invest Ophthalmol Vis Sci. 1982 Mar;22(3):376–381. [PubMed] [Google Scholar]
  15. Zimmermann D. R., Fischer R. W., Winterhalter K. H., Witmer R., Vaughan L. Comparative studies of collagens in normal and keratoconus corneas. Exp Eye Res. 1988 Mar;46(3):431–442. doi: 10.1016/s0014-4835(88)80031-9. [DOI] [PubMed] [Google Scholar]
  16. Zimmermann D. R., Trüeb B., Winterhalter K. H., Witmer R., Fischer R. W. Type VI collagen is a major component of the human cornea. FEBS Lett. 1986 Mar 3;197(1-2):55–58. doi: 10.1016/0014-5793(86)80297-6. [DOI] [PubMed] [Google Scholar]
  17. von der Mark H., Aumailley M., Wick G., Fleischmajer R., Timpl R. Immunochemistry, genuine size and tissue localization of collagen VI. Eur J Biochem. 1984 Aug 1;142(3):493–502. doi: 10.1111/j.1432-1033.1984.tb08313.x. [DOI] [PubMed] [Google Scholar]
  18. von der Mark K. Localization of collagen types in tissues. Int Rev Connect Tissue Res. 1981;9:265–324. doi: 10.1016/b978-0-12-363709-3.50012-7. [DOI] [PubMed] [Google Scholar]

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