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. 1991 Jul 1;277(Pt 1):239–243. doi: 10.1042/bj2770239

Kinetics of GTP hydrolysis during the assembly of chick brain MAP2-tubulin microtubule protein.

R G Burns 1
PMCID: PMC1151215  PMID: 1854336

Abstract

The kinetics of GTP hydrolysis during microtubule assembly have been examined using chick brain MAP2-tubulin microtubule protein in a NaCl-supplemented buffer. The elongating microtubules terminate in a 'GTP cap', since the kinetics of GTP hydrolysis are slower than those of subunit addition. GTP hydrolysis is (a) stoichiometric, (b) occurs as a vectorial wave as the initial rate of hydrolysis is proportional to the molar concentration of microtubule ends and not to the initial rate of subunit addition, and (c) either does not occur, or occurs only at a much lower rate, in the terminal subunits.

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Selected References

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