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. 1991 Jul 1;277(Pt 1):281–284. doi: 10.1042/bj2770281

Requirements for the inactivation of ribosomes by gelonin.

S Sperti 1, M Brigotti 1, M Zamboni 1, D Carnicelli 1, L Montanaro 1
PMCID: PMC1151221  PMID: 1854340

Abstract

Inactivation of Artemia salina and rabbit ribosomes by gelonin requires ATP and a high-Mr factor present in the rabbit reticulocyte-lysate post-ribosomal supernatant. The kinetic constants of the gelonin-catalysed release of adenine from A. salina ribosomes are Km = 4.35 microM and Kcat. = 0.1 min-1 in the absence of cofactors, and Km = 1.15 microM and Kcat. = 108 min-1 in their presence. The last two values are similar to those measured for ricin A chain in the absence of cofactors (Km = 2.02 microM and Kcat. = 317 min-1).

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Selected References

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