Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1991 Nov 1;279(Pt 3):633–641. doi: 10.1042/bj2790633

Characterization and purification of proteins which bind high-density lipoprotein. A putative cell-surface receptor.

H M Bond 1, G Morrone 1, S Venuta 1, K E Howell 1
PMCID: PMC1151492  PMID: 1659384

Abstract

High-density lipoprotein (HDL) is shown by ligand blotting to bind membrane-associated polypeptides with sizes of 60, 100 and 210 kDa. Binding was concentration-dependent and competed by excess unlabelled HDL. All the major apolipoproteins of HDL, apoA-I, apoA-II and apoA-IV, bound independently. The 100 kDa and 210 kDa HDL-binding activities were purified from membranes of Hep3B tumour cells by ion-exchange chromatography and gel filtration. The binding activities at 100 kDa and 210 kDa co-purified. After treatment with disulphide-reducing reagent, the 210 kDa band was no longer present and an increase was observed in the amount and binding ability of the 100 kDa polypeptide. The 100 kDa binding protein labelled at the cell surface with 125I could be immunoprecipitated after cross-linking to cell-surface-bound HDL. It is proposed that this HDL-binding activity, a putative cell-surface receptor for HDL, exists totally or in part as a high-molecular-mass complex composed of 100 kDa subunits.

Full text

PDF
638

Images in this article

635Fig. 1.
on p.635
635Fig. 2.
on p.635
636Fig. 3.
on p.636
637Fig. 4.
on p.637
638Fig. 5.
on p.638
639Fig. 6.
on p.639
640Fig. 7.
on p.640
640Fig. 8.
on p.640

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alam R., Yatsu F. M., Tsui L., Alam S. Receptor-mediated uptake and 'retroendocytosis' of high-density lipoproteins by cholesterol-loaded human monocyte-derived macrophages: possible role in enhancing reverse cholesterol transport. Biochim Biophys Acta. 1989 Aug 22;1004(3):292–299. doi: 10.1016/0005-2760(89)90076-3. [DOI] [PubMed] [Google Scholar]
  2. Barbaras R., Puchois P., Fruchart J. C., Pradines-Figueres A., Ailhaud G. Purification of an apolipoprotein A binding protein from mouse adipose cells. Biochem J. 1990 Aug 1;269(3):767–773. doi: 10.1042/bj2690767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barbaras R., Puchois P., Grimaldi P., Barkia A., Fruchart J. C., Ailhaud G. Relationship in adipose cells between the presence of receptor sites for high density lipoproteins and the promotion of reverse cholesterol transport. Biochem Biophys Res Commun. 1987 Dec 16;149(2):545–554. doi: 10.1016/0006-291x(87)90402-5. [DOI] [PubMed] [Google Scholar]
  4. Beisiegel U., Weber W., Havinga J. R., Ihrke G., Hui D. Y., Wernette-Hammond M. E., Turck C. W., Innerarity T. L., Mahley R. W. Apolipoprotein E-binding proteins isolated from dog and human liver. Arteriosclerosis. 1988 May-Jun;8(3):288–297. doi: 10.1161/01.atv.8.3.288. [DOI] [PubMed] [Google Scholar]
  5. Bierman E. L., Stein O., Stein Y. Lipoprotein uptake and metabolism by rat aortic smooth muscle cells in tissue culture. Circ Res. 1974 Jul;35(1):136–150. doi: 10.1161/01.res.35.1.136. [DOI] [PubMed] [Google Scholar]
  6. Boguski M. S., Elshourbagy N. A., Taylor J. M., Gordon J. I. Rat apolipoprotein A-IV: application of computational methods for studying the structure, function, and evolution of a protein. Methods Enzymol. 1986;128:753–773. doi: 10.1016/0076-6879(86)28104-5. [DOI] [PubMed] [Google Scholar]
  7. Daniel T. O., Schneider W. J., Goldstein J. L., Brown M. S. Visualization of lipoprotein receptors by ligand blotting. J Biol Chem. 1983 Apr 10;258(7):4606–4611. [PubMed] [Google Scholar]
  8. DeLamatre J. G., Sarphie T. G., Archibold R. C., Hornick C. A. Metabolism of apoE-free high density lipoproteins in rat hepatoma cells: evidence for a retroendocytic pathway. J Lipid Res. 1990 Feb;31(2):191–202. [PubMed] [Google Scholar]
  9. DeLamatre J., Wolfbauer G., Phillips M. C., Rothblat G. H. Role of apolipoproteins in cellular cholesterol efflux. Biochim Biophys Acta. 1986 Feb 28;875(3):419–428. doi: 10.1016/0005-2760(86)90061-5. [DOI] [PubMed] [Google Scholar]
  10. Fidge N. H., Nestel P. J. Identification of apolipoproteins involved in the interaction of human high density lipoprotein3 with receptors on cultured cells. J Biol Chem. 1985 Mar 25;260(6):3570–3575. [PubMed] [Google Scholar]
  11. Fidge N., Kagami A., O'Connor M. Identification of a high density lipoprotein binding protein from adrenocortical membranes. Biochem Biophys Res Commun. 1985 Jun 28;129(3):759–765. doi: 10.1016/0006-291x(85)91957-6. [DOI] [PubMed] [Google Scholar]
  12. Ghiselli G., Crump W., 3rd, Musanti R., Gotto A. M., Jr Identification of a detergent-solubilized plasma membrane protein from rat liver recognizing apolipoprotein A-IV. Biochim Biophys Acta. 1990 May 1;1044(1):173–175. doi: 10.1016/0005-2760(90)90234-o. [DOI] [PubMed] [Google Scholar]
  13. Graham D. L., Oram J. F. Identification and characterization of a high density lipoprotein-binding protein in cell membranes by ligand blotting. J Biol Chem. 1987 Jun 5;262(16):7439–7442. [PubMed] [Google Scholar]
  14. Havekes L., Schouten D., van Hinsbergh V., de Wit E. Characterisation of the binding of apolipoprotein E-free high density lipoprotein to cultured human endothelial cells. Biochem Biophys Res Commun. 1984 Jul 31;122(2):785–790. doi: 10.1016/s0006-291x(84)80102-3. [DOI] [PubMed] [Google Scholar]
  15. Hwang J., Menon K. M. Binding of apolipoprotein A-I and A-II after recombination with phospholipid vesicles to the high density lipoprotein receptor of luteinized rat ovary. J Biol Chem. 1985 May 10;260(9):5660–5668. [PubMed] [Google Scholar]
  16. Karlin J. B., Johnson W. J., Benedict C. R., Chacko G. K., Phillips M. C., Rothblat G. H. Cholesterol flux between cells and high density lipoprotein. Lack of relationship to specific binding of the lipoprotein to the cell surface. J Biol Chem. 1987 Sep 15;262(26):12557–12564. [PubMed] [Google Scholar]
  17. Keso L., Lukka M., Ehnholm C., Baumann M., Vihko P., Olkinuora M. Apolipoprotein A-I-binding protein from human term placenta. Purification and partial characterization. FEBS Lett. 1987 May 4;215(1):105–108. doi: 10.1016/0014-5793(87)80122-9. [DOI] [PubMed] [Google Scholar]
  18. Lorenzetti R., Sidoli A., Palomba R., Monaco L., Martineau D., Lappi D. A., Soria M. Expression of the human apolipoprotein AI gene fused to the E. coli gene for beta-galactosidase. FEBS Lett. 1986 Jan 6;194(2):343–346. doi: 10.1016/0014-5793(86)80114-4. [DOI] [PubMed] [Google Scholar]
  19. Louvard D., Reggio H., Warren G. Antibodies to the Golgi complex and the rough endoplasmic reticulum. J Cell Biol. 1982 Jan;92(1):92–107. doi: 10.1083/jcb.92.1.92. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mendel C. M., Kunitake S. T., Kane J. P., Kempner E. S. Radiation inactivation of binding sites for high density lipoproteins in human fibroblast membranes. J Biol Chem. 1988 Jan 25;263(3):1314–1319. [PubMed] [Google Scholar]
  21. Monaco L., Bond H. M., Howell K. E., Cortese R. A recombinant apoA-1--protein A hybrid reproduces the binding parameters of HDL to its receptor. EMBO J. 1987 Nov;6(11):3253–3260. doi: 10.1002/j.1460-2075.1987.tb02643.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nilsson B., Abrahmsén L., Uhlén M. Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors. EMBO J. 1985 Apr;4(4):1075–1080. doi: 10.1002/j.1460-2075.1985.tb03741.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Oppenheimer M. J., Oram J. F., Bierman E. L. Up-regulation of high density lipoprotein receptor activity by gamma-interferon associated with inhibition of cell proliferation. J Biol Chem. 1988 Dec 25;263(36):19318–19323. [PubMed] [Google Scholar]
  24. Phillips M. C., Johnson W. J., Rothblat G. H. Mechanisms and consequences of cellular cholesterol exchange and transfer. Biochim Biophys Acta. 1987 Jun 24;906(2):223–276. doi: 10.1016/0304-4157(87)90013-x. [DOI] [PubMed] [Google Scholar]
  25. Rifici V. A., Eder H. A. A hepatocyte receptor for high-density lipoproteins specific for apolipoprotein A-I. J Biol Chem. 1984 Nov 25;259(22):13814–13818. [PubMed] [Google Scholar]
  26. Schmitz G., Niemann R., Brennhausen B., Krause R., Assmann G. Regulation of high density lipoprotein receptors in cultured macrophages: role of acyl-CoA:cholesterol acyltransferase. EMBO J. 1985 Nov;4(11):2773–2779. doi: 10.1002/j.1460-2075.1985.tb04003.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Schmitz G., Robenek H., Lohmann U., Assmann G. Interaction of high density lipoproteins with cholesteryl ester-laden macrophages: biochemical and morphological characterization of cell surface receptor binding, endocytosis and resecretion of high density lipoproteins by macrophages. EMBO J. 1985 Mar;4(3):613–622. doi: 10.1002/j.1460-2075.1985.tb03674.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schumaker V. N., Puppione D. L. Sequential flotation ultracentrifugation. Methods Enzymol. 1986;128:155–170. doi: 10.1016/0076-6879(86)28066-0. [DOI] [PubMed] [Google Scholar]
  29. Slotte J. P., Oram J. F., Bierman E. L. Binding of high density lipoproteins to cell receptors promotes translocation of cholesterol from intracellular membranes to the cell surface. J Biol Chem. 1987 Sep 25;262(27):12904–12907. [PubMed] [Google Scholar]
  30. Stein O., Stein Y., Lefevre M., Roheim P. S. The role of apolipoprotein A-IV in reverse cholesterol transport studied with cultured cells and liposomes derived from an ether analog of phosphatidylcholine. Biochim Biophys Acta. 1986 Aug 14;878(1):7–13. doi: 10.1016/0005-2760(86)90337-1. [DOI] [PubMed] [Google Scholar]
  31. Tozuka M., Fidge N. Purification and characterization of two high-density-lipoprotein-binding proteins from rat and human liver. Biochem J. 1989 Jul 1;261(1):239–244. doi: 10.1042/bj2610239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Vadiveloo P. K., Fidge N. H. Studies on the interaction between apolipoprotein A-II-enriched HDL3 and cultured bovine aortic endothelial (BAE) cells. Biochim Biophys Acta. 1990 Jul 16;1045(2):135–141. doi: 10.1016/0005-2760(90)90142-k. [DOI] [PubMed] [Google Scholar]
  33. Wetterau J. R., Combs K. A., Spinner S. N., Joiner B. J. Protein disulfide isomerase is a component of the microsomal triglyceride transfer protein complex. J Biol Chem. 1990 Jun 15;265(17):9800–9807. [PubMed] [Google Scholar]
  34. van Driel I. R., Davis C. G., Goldstein J. L., Brown M. S. Self-association of the low density lipoprotein receptor mediated by the cytoplasmic domain. J Biol Chem. 1987 Nov 25;262(33):16127–16134. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES