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. 1983 Apr 15;212(1):189–195. doi: 10.1042/bj2120189

Glucagon inhibition of insulin-stimulated 2-deoxyglucose uptake by rat adipocytes in the presence of adenosine deaminase.

A Green
PMCID: PMC1152028  PMID: 6347192

Abstract

Effects of adenosine deaminase and glucagon on insulin-stimulated 2-deoxyglucose uptake by rat adipocytes are reported. (1) Adenosine deaminase (10 micrograms/ml) caused a rightward shift in the dose-response curve for the stimulation by insulin of 2-deoxyglucose uptake, but the enzyme did not alter either the basal or the maximally insulin-stimulated uptake rate. (2) In adipocytes obtained from 24 h-starved rats, glucagon inhibited the effect of insulin on 2-deoxyglucose uptake in the presence (but not in the absence) of adenosine deaminase. Basal uptake rates were unaffected. (3) Glucagon inhibited insulin-stimulated 2-deoxyglucose uptake to a greater extent in cells isolated from starved rats than in cells from fed rats. (4) Adipocytes isolated from fed and from starved rats did not differ in their capacity for degradation of 125I-labelled glucagon. The results suggest that adenosine and glucagon are regulators of insulin action in adipose tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alford F. P., Chisholm D. J. Glucagon--new concepts about and "old" hormone. Aust N Z J Med. 1979 Dec;9(6):733–743. doi: 10.1111/j.1445-5994.1979.tb04210.x. [DOI] [PubMed] [Google Scholar]
  2. Blecher M., Merlino N. S., Ro'Ane J. T., Flynn P. D. Independence of the effects of epinephrine, glucagon, and adrenocorticotropin on glucose utilization from those on lipolysis in isolated rat adipose cells. J Biol Chem. 1969 Jul 10;244(13):3423–3429. [PubMed] [Google Scholar]
  3. Caro J. F., Amatruda J. M. The effects of fasting on glucagon binding and action in isolated rat hepatocytes. Metabolism. 1980 Aug;29(8):732–738. doi: 10.1016/0026-0495(80)90195-x. [DOI] [PubMed] [Google Scholar]
  4. Fernandez B. M., Saggerson E. D. Alterations in response of rat white adipocytes to insulin, noradrenaline, corticotropin and glucagon after adrenalectomy. Correction of these changes by adenosine deaminase. Biochem J. 1978 Jul 15;174(1):111–118. doi: 10.1042/bj1740111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fisher M., Sherwin R. S., Hendler R., Felig P. Kinetics of glucagon in man: effects of starvation. Proc Natl Acad Sci U S A. 1976 May;73(5):1735–1739. doi: 10.1073/pnas.73.5.1735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Green A., Fisher M., Newsholme E. A. Maximum activities of enzymes involved in adenosine metabolism in adipose tissue of rats and mice under conditions of variations in insulin sensitivity. Biochim Biophys Acta. 1981 Aug 5;676(1):125–128. doi: 10.1016/0304-4165(81)90018-0. [DOI] [PubMed] [Google Scholar]
  7. Green A., Newsholme E. A. Sensitivity of glucose uptake and lipolysis of white adipocytes of the rat to insulin and effects of some metabolites. Biochem J. 1979 May 15;180(2):365–370. doi: 10.1042/bj1800365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  9. Helman A. M., Amira R., Nicolaïdis S., Assan R. Glucagon release induced by ventrolateral hypothalamic stimulation in the rat. Endocrinology. 1980 May;106(5):1612–1619. doi: 10.1210/endo-106-5-1612. [DOI] [PubMed] [Google Scholar]
  10. Hirsch J., Gallian E. Methods for the determination of adipose cell size in man and animals. J Lipid Res. 1968 Jan;9(1):110–119. [PubMed] [Google Scholar]
  11. Honnor R. C., Saggerson E. D. Altered lipolytic response to glucagon and adenosine deaminase in adipocytes from starved rats. Biochem J. 1980 Jun 15;188(3):757–761. doi: 10.1042/bj1880757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lefebvre P. Glucagon and adipose tissue. Biochem Pharmacol. 1975 Jul 15;24(13-14):1261–1266. doi: 10.1016/0006-2952(75)90333-0. [DOI] [PubMed] [Google Scholar]
  13. Marliss E. B., Aoki T. T., Unger R. H., Soeldner J. S., Cahill G. F., Jr Glucagon levels and metabolic effects in fasting man. J Clin Invest. 1970 Dec;49(12):2256–2270. doi: 10.1172/JCI106445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Olefsky J. M. Effects of fasting on insulin binding, glucose transport, and glucose oxidation in isolated rat adipocytes: relationships between insulin receptors and insulin action. J Clin Invest. 1976 Dec;58(6):1450–1460. doi: 10.1172/JCI108601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Olefsky J. M. Mechanisms of the ability of insulin to activate the glucose-transport system in rat adipocytes. Biochem J. 1978 Apr 15;172(1):137–145. doi: 10.1042/bj1720137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Perez-Castillo A., Blázquez E. Tissue distribution of glucagon, glucagonlike immunoreactivity, and insulin in the rat. Am J Physiol. 1980 Mar;238(3):E258–E266. doi: 10.1152/ajpendo.1980.238.3.E258. [DOI] [PubMed] [Google Scholar]
  17. RODBELL M. METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS. J Biol Chem. 1964 Feb;239:375–380. [PubMed] [Google Scholar]
  18. Schwabe U., Ebert R., Erbler H. C. Adenosine release from fat cells: effect on cyclic AMP levels and hormone actions. Adv Cyclic Nucleotide Res. 1975;5:569–584. [PubMed] [Google Scholar]
  19. Schwabe U., Ebert R., Erbler H. C. Adenosine release from isolated fat cells and its significance for the effects of hormones on cyclic 3',5'-AMP levels and lipolysis. Naunyn Schmiedebergs Arch Pharmacol. 1973;276(2):133–148. doi: 10.1007/BF00501186. [DOI] [PubMed] [Google Scholar]
  20. Sonne O., Gliemann J. Receptor binding of glucagon and adenosine 3',5'-monophosphate accumulation in isolated rat fat cells. Biochim Biophys Acta. 1977 Sep 29;499(2):259–272. doi: 10.1016/0304-4165(77)90008-3. [DOI] [PubMed] [Google Scholar]
  21. Unger R. H., Orci L. Glucagon and the A cell: physiology and pathophysiology (first two parts). N Engl J Med. 1981 Jun 18;304(25):1518–1524. doi: 10.1056/NEJM198106183042504. [DOI] [PubMed] [Google Scholar]
  22. Woodson L. C., Potter D. E. The influence of endogenous glucagon release on hyperglycemic responses to catecholamines in normal fed and diabetic rats. J Pharmacol Exp Ther. 1979 Sep;210(3):458–464. [PubMed] [Google Scholar]

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