Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1983 Aug 1;213(2):297–304. doi: 10.1042/bj2130297

Evidence for the presence and structure of asparagine-linked oligosaccharide units in the core protein of proteodermatan sulphate.

H Shinkai, T Nakamura, E Matsunaga
PMCID: PMC1152128  PMID: 6615437

Abstract

The N-glycosidically linked oligosaccharides were liberated by hydrazinolysis from purified proteodermatan sulphate from newborn-calf skin and reduced with NaB3H4 at the reducing terminal sugar. One asparagine-linked oligosaccharide chain was linked to one core-protein molecule in proteodermatan sulphate. Structural sequences were analysed by using exoglycosidase digestion. These oligosaccharides were composed of di- and tri-antennary oligosaccharide structures of complex type.

Full text

PDF
300

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arakawa M., Ogata S., Muramatsu T., Kobata A. Beta-galactosidases from jack bean meal and almond emulsin. Application for the enzymatic distinction of Galbeta1 leads to 4GlcNAc and Galbeta1 leads to 3GlcNAc linkages. J Biochem. 1974 Apr;75(4):707–714. doi: 10.1093/oxfordjournals.jbchem.a130443. [DOI] [PubMed] [Google Scholar]
  2. Cöster L., Fransson L. A. Isolation and characterization of dermatan sulphate proteoglycans from bovine sclera. Biochem J. 1981 Jan 1;193(1):143–153. doi: 10.1042/bj1930143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. De Luca S., Lohmander L. S., Nilsson B., Hascall V. C., Caplan A. I. Proteoglycans from chick limb bud chondrocyte cultures. Keratan sulfate and oligosaccharides which contain mannose and sialic acid. J Biol Chem. 1980 Jul 10;255(13):6077–6083. [PubMed] [Google Scholar]
  4. Fleischmajer R., Perlish J. S., Bashey R. I. Human dermal glycosaminoglycans and aging. Biochim Biophys Acta. 1972 Sep 15;279(2):265–275. doi: 10.1016/0304-4165(72)90142-0. [DOI] [PubMed] [Google Scholar]
  5. Fujii N., Nagai Y. Isolation and characterization of a proteodermatan sulfate from calf skin. J Biochem. 1981 Nov;90(5):1249–1258. doi: 10.1093/oxfordjournals.jbchem.a133589. [DOI] [PubMed] [Google Scholar]
  6. HUGHES R. C., JEANLOZ R. W. THE EXTRACELLULAR GLYCOSIDASES OF DIPLOCOCCUS PNEUMONIAE. I. PURIFICATION AND PROPERTIES OF A NEURAMINIDASE AND A BETA-GALACTOSIDASE. ACTION ON THE ALPHA-1-ACID GLYCOPROTEIN OF HUMAN PLASMA. Biochemistry. 1964 Oct;3:1535–1543. doi: 10.1021/bi00898a025. [DOI] [PubMed] [Google Scholar]
  7. Kobata A. Use of endo- and exoglycosidases for structural studies of glycoconjugates. Anal Biochem. 1979 Nov 15;100(1):1–14. doi: 10.1016/0003-2697(79)90102-7. [DOI] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Lohmander L. S., De Luca S., Nilsson B., Hascall V. C., Caputo C. B., Kimura J. H., Heinegard D. Oligosaccharides on proteoglycans from the swarm rat chondrosarcoma. J Biol Chem. 1980 Jul 10;255(13):6084–6091. [PubMed] [Google Scholar]
  10. Mizuochi T., Taniguchi T., Fujii-Kadowaki J., Yonemasu K., Sasaki T., Kobata A. Structures of the asparagine-linked sugar chains of subcomponent C1q of the first component of bovine complement. J Biol Chem. 1982 Nov 25;257(22):13300–13305. [PubMed] [Google Scholar]
  11. Mizuochi T., Yonemasu K., Yamashita K., Kobata A. The asparagine-linked sugar chains of subcomponent C1q of the first component of human complement. J Biol Chem. 1978 Oct 25;253(20):7404–7409. [PubMed] [Google Scholar]
  12. Nakamura T., Matsunaga E., Shinkai H. Isolation and some structural analyses of a proteodermatan sulphate from calf skin. Biochem J. 1983 Aug 1;213(2):289–296. doi: 10.1042/bj2130289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nilsson B., De Luca S., Lohmander S., Hascall V. C. Structures of N-linked and O-linked oligosaccharides on proteoglycan monomer isolated from the Swarm rat chondrosarcoma. J Biol Chem. 1982 Sep 25;257(18):10920–10927. [PubMed] [Google Scholar]
  14. Nishigaki M., Yamashita K., Matsuda I., Arashima S., Kobata A. Urinary oligosaccharides of fucosidosis. Evidence of the occurrence of X-antigenic determinant in serum-type sugar chains of glycoproteins. J Biochem. 1978 Oct;84(4):823–834. doi: 10.1093/oxfordjournals.jbchem.a132194. [DOI] [PubMed] [Google Scholar]
  15. Scott J. E., Orford C. R. Dermatan sulphate-rich proteoglycan associates with rat tail-tendon collagen at the d band in the gap region. Biochem J. 1981 Jul 1;197(1):213–216. doi: 10.1042/bj1970213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Takasaki S., Kobata A. Microdetermination of individual neutral and amino sugars and N-acetylneuraminic acid in complex saccharides. J Biochem. 1974 Oct;76(4):783–789. [PubMed] [Google Scholar]
  17. Yoshima H., Takasaki S., Kobata A. The asparagine-linked sugar chains of the glycoproteins in calf thymocyte plasma membrane. Structural studies of acidic oligosaccharides. J Biol Chem. 1980 Nov 25;255(22):10793–10804. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES