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. 1983 Jul 15;214(1):215–223. doi: 10.1042/bj2140215

The effects of corticosterone, cold exposure and overfeeding with sucrose on brown adipose tissue of obese Zucker rats (fa/fa).

S Holt, D A York, J T Fitzsimons
PMCID: PMC1152229  PMID: 6615467

Abstract

GDP binding to brown-adipose-tissue mitochondria was decreased in obese Zucker rats. Adrenalectomy restored both GDP binding and serum tri-iodothyronine of obese rats to values observed in lean rats. The effects of adrenalectomy on GDP binding and serum tri-iodothyronine were reversed by corticosterone. Decreasing food intake had no effect on brown-adipose-tissue GDP binding in obese rats. Young (5-week-old) obese rats showed a normal increase in brown-adipose-tissue mitochondrial GDP binding after housing at 4 degrees C for 7 days, but this response was attenuated in 10-week-old obese rats. Overfeeding with sucrose increased brown-adipose-tissue thermogenesis in lean, but not in obese, rats. After adrenalectomy, overfeeding with sucrose enhanced brown-adipose-tissue mitochondrial GDP binding in obese rats.

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Selected References

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  1. Autissier N., Dumas P., Loireau A., Michel R. Thyroid status and effects of 3,5,3' triiodothyroacetic acid and fenproporex in genetically lean and obese female rats. Biochem Pharmacol. 1980 Jun 1;29(11):1612–1615. doi: 10.1016/0006-2952(80)90621-8. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Bray G. A. Regulation of energy balance: studies on genetic, hypothalamic and dietary obesity. Proc Nutr Soc. 1982 Jun;41(2):95–108. doi: 10.1079/pns19820018. [DOI] [PubMed] [Google Scholar]
  4. Bray G. A., York D. A. Hypothalamic and genetic obesity in experimental animals: an autonomic and endocrine hypothesis. Physiol Rev. 1979 Jul;59(3):719–809. doi: 10.1152/physrev.1979.59.3.719. [DOI] [PubMed] [Google Scholar]
  5. Bray G. A., York D. A., Swerloff R. S. Genetic obesity in rats. I. The effects of food restriction on body composition and hypothalamic function. Metabolism. 1973 Mar;22(3):435–442. doi: 10.1016/0026-0495(73)90035-8. [DOI] [PubMed] [Google Scholar]
  6. Bray G. A., York D. A. Thyroid function of genetically obese rats. Endocrinology. 1971 Apr;88(4):1095–1099. doi: 10.1210/endo-88-4-1095. [DOI] [PubMed] [Google Scholar]
  7. Brooks S. L., Rothwell N. J., Stock M. J., Goodbody A. E., Trayhurn P. Increased proton conductance pathway in brown adipose tissue mitochondria of rats exhibiting diet-induced thermogenesis. Nature. 1980 Jul 17;286(5770):274–276. doi: 10.1038/286274a0. [DOI] [PubMed] [Google Scholar]
  8. Bruce B. K., King B. M., Phelps G. R., Veitia M. C. Effects of adrenalectomy and corticosterone administration on hypothalamic obesity in rats. Am J Physiol. 1982 Aug;243(2):E152–E157. doi: 10.1152/ajpendo.1982.243.2.E152. [DOI] [PubMed] [Google Scholar]
  9. Carlson H. E., Drenick E. J., Chopra I. J., Hershman J. M. Alterations in basal and TRH-stimulated serum levels of thyrotropin, prolactin, and thyroid hormones in starved obese men. J Clin Endocrinol Metab. 1977 Oct;45(4):707–713. doi: 10.1210/jcem-45-4-707. [DOI] [PubMed] [Google Scholar]
  10. Desautels M., Zaror-Behrens G., Himms-Hagen J. Increased purine nucleotide binding, altered polypeptide composition, and thermogenesis in brown adipose tissue mitochondria of cold-acclimated rats. Can J Biochem. 1978 Jun;56(6):378–383. doi: 10.1139/o78-060. [DOI] [PubMed] [Google Scholar]
  11. Fellenz M., Triandafillou J., Gwilliam C., Himms-Hagen J. Growth of interscapular brown adipose tissue in cold-acclimated hypophysectomized rats maintained on thyroxine and corticosterone. Can J Biochem. 1982 Aug;60(8):838–842. doi: 10.1139/o82-105. [DOI] [PubMed] [Google Scholar]
  12. Godbole V., York D. A., Bloxham D. P. Developmental changes in the fatty (fafa) rat: evidence for defective thermogenesis preceding the hyperlipogenesis and hyperinsulinaemia. Diabetologia. 1978 Jul;15(1):41–44. doi: 10.1007/BF01219327. [DOI] [PubMed] [Google Scholar]
  13. Goodbody A. E., Trayhurn P. GDP binding to brown-adipose-tissue mitochondria of diabetic--obese (db/db) mice. Decreased binding in both the obese and pre-obese states. Biochem J. 1981 Mar 15;194(3):1019–1022. doi: 10.1042/bj1941019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Himms-Hagen J. Cellular thermogenesis. Annu Rev Physiol. 1976;38:315–351. doi: 10.1146/annurev.ph.38.030176.001531. [DOI] [PubMed] [Google Scholar]
  15. Himms-Hagen J., Desautels M. A mitochondrial defect in brown adipose tissue of the obese (ob/ob) mouse: reduced binding of purine nucleotides and a failure to respond to cold by an increase in binding. Biochem Biophys Res Commun. 1978 Jul 28;83(2):628–634. doi: 10.1016/0006-291x(78)91036-7. [DOI] [PubMed] [Google Scholar]
  16. Hogan S., Coscina D. V., Himms-Hagen J. Brown adipose tissue of rats with obesity-inducing ventromedial hypothalamic lesions. Am J Physiol. 1982 Oct;243(4):E338–E344. doi: 10.1152/ajpendo.1982.243.4.E338. [DOI] [PubMed] [Google Scholar]
  17. Hogan S., Himms-Hagen J. Abnormal brown adipose tissue in obese (ob/ob) mice: response to acclimation to cold. Am J Physiol. 1980 Oct;239(4):E301–E309. doi: 10.1152/ajpendo.1980.239.4.E301. [DOI] [PubMed] [Google Scholar]
  18. Holt S., York D. A. The effect of adrenalectomy on GDP binding to brown-adipose-tissue mitochondria of obese rat. Biochem J. 1982 Dec 15;208(3):819–822. doi: 10.1042/bj2080819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Landsberg L., Young J. B. Effects of nutritional status on autonomic nervous system function. Am J Clin Nutr. 1982 May;35(5 Suppl):1234–1240. doi: 10.1093/ajcn/35.5.1234. [DOI] [PubMed] [Google Scholar]
  20. Lavau M., Bazin R., Karaoghlanian Z., Guichard C. Evidence for a high fatty acid synthesis activity in interscapular brown adipose tissue of genetically obese Zucker rats. Biochem J. 1982 May 15;204(2):503–507. doi: 10.1042/bj2040503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Leblanc J., Villemaire A. Throxine and noradrenaline on noradrenaline sensitivity, cold resistance, and brown fat. Am J Physiol. 1970 Jun;218(6):1742–1745. doi: 10.1152/ajplegacy.1970.218.6.1742. [DOI] [PubMed] [Google Scholar]
  22. Levin B. E., Comai K., O'Brien R. A., Sullivan A. C. Abnormal brown adipose composition and beta-adrenoceptor binding in obese Zucker rats. Am J Physiol. 1982 Sep;243(3):E217–E224. doi: 10.1152/ajpendo.1982.243.3.E217. [DOI] [PubMed] [Google Scholar]
  23. Levin B. E., Triscari J., Sullivan A. C. Abnormal sympatho- adrenal function and plasma catecholamines in obese Zucker rats. Pharmacol Biochem Behav. 1980 Jul;13(1):107–113. doi: 10.1016/0091-3057(80)90128-8. [DOI] [PubMed] [Google Scholar]
  24. Martin R. J., Wangsness P. J., Gahagan J. H. Diurnal changes in serum metabolites and hormones in lean and obese Zucker rats. Horm Metab Res. 1978 May;10(3):187–192. doi: 10.1055/s-0028-1093431. [DOI] [PubMed] [Google Scholar]
  25. Nicholls D. G. Brown adipose tissue mitochondria. Biochim Biophys Acta. 1979 Jul 3;549(1):1–29. doi: 10.1016/0304-4173(79)90016-8. [DOI] [PubMed] [Google Scholar]
  26. Pamenter R. W., Hedge G. A. Inhibition of thyrotropin secretion by physiological levels of corticosterone. Endocrinology. 1980 Jan;106(1):162–166. doi: 10.1210/endo-106-1-162. [DOI] [PubMed] [Google Scholar]
  27. Powley T. L., Morton S. A. Hypophysectomy and regulation of body weight in the genetically obese Zucker rat. Am J Physiol. 1976 Apr;230(4):982–987. doi: 10.1152/ajplegacy.1976.230.4.982. [DOI] [PubMed] [Google Scholar]
  28. Radcliffe J. D., Webster A. J. Regulation of food intake during growth in fatty and lean female Zucker rats given diets of different protein content. Br J Nutr. 1976 Nov;36(3):457–469. doi: 10.1079/bjn19760100. [DOI] [PubMed] [Google Scholar]
  29. Rothwell N. J., Stock M. J. A role for brown adipose tissue in diet-induced thermogenesis. Nature. 1979 Sep 6;281(5726):31–35. doi: 10.1038/281031a0. [DOI] [PubMed] [Google Scholar]
  30. Rothwell N. J., Stock M. J. Effects of feeding a palatable "cafeteria' diet on energy balance in young and adult lean (+/?) Zucker rats. Br J Nutr. 1982 May;47(3):461–471. doi: 10.1079/bjn19820058. [DOI] [PubMed] [Google Scholar]
  31. Rothwell N. J., Stock M. J., Tyzbir R. S. Energy balance and mitochondrial function in liver and brown fat of rats fed "cafeteria" diets of varying protein content. J Nutr. 1982 Sep;112(9):1663–1672. doi: 10.1093/jn/112.9.1663. [DOI] [PubMed] [Google Scholar]
  32. Schanbacher B. D., Crouse J. D. Photoperiodic regulation of growth: a photosensitive phase during light-dark cycle. Am J Physiol. 1981 Jul;241(1):E1–E5. doi: 10.1152/ajpendo.1981.241.1.E1. [DOI] [PubMed] [Google Scholar]
  33. Seydoux J., Ricquier D., Rohner-Jeanreanaud F., Assimacopoulos-Jeannet F., Giacobino J. P., Jeanrenaud B., Girardier L. Decreased guanine nucleotide binding and reduced equivalent production by brown adipose tissue in hypothalamic obesity. Recovery after cold acclimation. FEBS Lett. 1982 Sep 6;146(1):161–164. doi: 10.1016/0014-5793(82)80726-6. [DOI] [PubMed] [Google Scholar]
  34. Slinde E., Pedersen J. I., Flatmark T. Sedimentation coefficient and buoyant density of brown adipose tissue mitochondria from guinea pigs. Anal Biochem. 1975 May 12;65(1-2):581–585. doi: 10.1016/0003-2697(75)90551-5. [DOI] [PubMed] [Google Scholar]
  35. Trayhurn P., James W. P. Thermoregulation and non-shivering thermogenesis in the genetically obese (ob/ob) mouse. Pflugers Arch. 1978 Feb 22;373(2):189–193. doi: 10.1007/BF00584859. [DOI] [PubMed] [Google Scholar]
  36. Trayhurn P., Jones P. M., McGuckin M. M., Goodbody A. E. Effects of overfeeding on energy balance and brown fat thermogenesis in obese (ob/ob) mice. Nature. 1982 Jan 28;295(5847):323–325. doi: 10.1038/295323a0. [DOI] [PubMed] [Google Scholar]
  37. Triandafillou J., Gwilliam C., Himms-Hagen J. Role of thyroid hormone in cold-induced changes in rat brown adipose tissue mitochondria. Can J Biochem. 1982 May;60(5):530–537. doi: 10.1139/o82-065. [DOI] [PubMed] [Google Scholar]
  38. York D. A., Godbole V. Effect of adrenalectomy on obese 'fatty' rats. Horm Metab Res. 1979 Nov;11(11):646–646. doi: 10.1055/s-0028-1095808. [DOI] [PubMed] [Google Scholar]
  39. York D. A., Hershman J. M., Utiger R. D., Bray G. A. Thyrotropin secretion in genetically obese rats. Endocrinology. 1972 Jan;90(1):67–72. doi: 10.1210/endo-90-1-67. [DOI] [PubMed] [Google Scholar]
  40. Yukimura Y., Bray G. A. Effects of adrenalectomy on body weight and the size and number of fat cells in the Zucker (fatty) rat. Endocr Res Commun. 1978;5(3):189–198. doi: 10.1080/07435807809083752. [DOI] [PubMed] [Google Scholar]
  41. Yukimura Y., Bray G. A., Wolfsen A. R. Some effects of adrenalectomy in the fatty rat. Endocrinology. 1978 Nov;103(5):1924–1928. doi: 10.1210/endo-103-5-1924. [DOI] [PubMed] [Google Scholar]

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