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. 1983 Oct 15;216(1):207–213. doi: 10.1042/bj2160207

Large effects of preparative techniques on lymphocyte cyclic AMP content.

J P Moore, G A Smith, T R Hesketh, J C Metcalfe
PMCID: PMC1152488  PMID: 6316936

Abstract

When a cell suspension is formed by disruption of a pig lymph node into medium, large and transient increases in intracellular cyclic AMP occur. Similar effects are observed when pig lymphocytes are centrifuged and the cell pellets resuspended, or when the cells are subjected to rapid temperature changes. These observations define the conditions required to manipulate the cells while maintaining a stable cyclic AMP concentration. Under these conditions, neither concanavalin A nor ionophore A23187 at mitogenic concentrations have any early effect on cyclic AMP in pig lymphocytes, but small increases in cyclic AMP (less than 2-fold) were observed at supramitogenic concentrations of concanavalin A (50 microgram/ml) or A23187 (500nM). Mouse thymocytes show qualitatively similar but much smaller changes in cyclic AMP concentration in response to experimental manipulations, and no response to mitogenic or supramitogenic concentrations of concanavalin A below the cytotoxic value.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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