Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1983 Dec 15;216(3):675–680. doi: 10.1042/bj2160675

Beta-adrenergic control of phosphatidylcholine synthesis by transmethylation in hepatocytes from juvenile, adult and adrenalectomized rats.

D Marin-Cao, V Alvarez Chiva, J M Mato
PMCID: PMC1152561  PMID: 6320796

Abstract

Changes in isoprenaline-sensitive phospholipid methyltransferase were studied in hepatocytes isolated from juvenile, mature and adrenalectomized rats. Isoprenaline produced greater stimulation of cyclic AMP accumulation in juvenile and mature adrenalectomized rats than in mature animals. Similarly, isoprenaline stimulated phospholipid methyltransferase in juvenile and mature adrenalectomized rats but had no effect in mature animals. Isoprenaline-mediated activation of phospholipid methyltransferase in adrenalectomized rats was time- and dose-dependent. In hepatocytes isolated from adrenalectomized rats incubated with [Me-3H]methionine or [3H]-ethanolamine the addition of isoprenaline increased the amount of radioactivity incorporated into phosphatidylcholine. The activation by isoprenaline of phospholipid methyltransferase was abolished by the beta-blocker propranolol and by insulin. These results indicate that rat liver the occupation of functional beta-receptors causes a stimulation of phospholipid methylation. It is suggested that, as reported previously, cyclic AMP activates phospholipid methyltransferase.

Full text

PDF
680

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alemany S., Varela I., Harper J. F., Mato J. M. Calmodulin regulation of phospholipid and fatty acid methylation by rat liver microsomes. J Biol Chem. 1982 Aug 25;257(16):9249–9251. [PubMed] [Google Scholar]
  2. Alemany S., Varela I., Mato J. M. Inhibition of phosphatidylcholine synthesis by vasopressin and angiotensin in rat hepatocytes. Biochem J. 1982 Nov 15;208(2):453–457. doi: 10.1042/bj2080453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alemany S., Varela I., Mato J. M. Stimulation by vasopressin and angiotensin of phospholipid methyltransferase in isolated rat hepatocytes. FEBS Lett. 1981 Nov 30;135(1):111–114. doi: 10.1016/0014-5793(81)80955-6. [DOI] [PubMed] [Google Scholar]
  4. Alonso F., Sánchez-Crespo M., Mato J. M. Modulatory role of cyclic AMP in the release of platelet-activating factor from human polymorphonuclear leucocytes. Immunology. 1982 Mar;45(3):493–500. [PMC free article] [PubMed] [Google Scholar]
  5. Blair J. B., James M. E., Foster J. L. Adrenergic control of glucose output and adenosine 3':5'-monophosphate levels in hepatocytes from juvenile and adult rats. J Biol Chem. 1979 Aug 25;254(16):7579–7584. [PubMed] [Google Scholar]
  6. Castaño J. G., Alemany S., Nieto A., Mato J. M. Activation of phospholipid methyltransferase by glucagon in rat hepatocytes. J Biol Chem. 1980 Oct 10;255(19):9041–9043. [PubMed] [Google Scholar]
  7. Chan T. M., Blackmore P. F., Steiner K. E., Exton J. H. Effects of adrenalectomy on hormone action on hepatic glucose metabolism. Reciprocal change in alpha- and beta-adrenergic activation of hepatic glycogen phosphorylase and calcium mobilization in adrenalectomized rats. J Biol Chem. 1979 Apr 10;254(7):2428–2433. [PubMed] [Google Scholar]
  8. Claus T. H., Pilkis S. J. Regulation by insulin of gluconeogenesis in isolated rat hepatocytes. Biochim Biophys Acta. 1976 Feb 24;421(2):246–262. doi: 10.1016/0304-4165(76)90291-9. [DOI] [PubMed] [Google Scholar]
  9. GIBSON K. D., WILSON J. D., UDENFRIEND S. The enzymatic conversion of phospholipid ethanolamine to phospholipid choline in rat liver. J Biol Chem. 1961 Mar;236:673–679. [PubMed] [Google Scholar]
  10. Geelen M. J., Groener J. E., De Haas C. G., Van Golde L. M. Influence of glucagon on the synthesis of phosphatidylcholines and phosphatidylethanolamines in monolayer cultures of rat hepatocytes. FEBS Lett. 1979 Sep 1;105(1):27–30. doi: 10.1016/0014-5793(79)80880-7. [DOI] [PubMed] [Google Scholar]
  11. Hirata F., Axelrod J. Phospholipid methylation and biological signal transmission. Science. 1980 Sep 5;209(4461):1082–1090. doi: 10.1126/science.6157192. [DOI] [PubMed] [Google Scholar]
  12. Hirata F., Strittmatter W. J., Axelrod J. beta-Adrenergic receptor agonists increase phospholipid methylation, membrane fluidity, and beta-adrenergic receptor-adenylate cyclase coupling. Proc Natl Acad Sci U S A. 1979 Jan;76(1):368–372. doi: 10.1073/pnas.76.1.368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hoffman D. R., Cornatzer W. E. Microsomal phosphatidylethanolamine methyltransferase: some physical and kinetic properties. Lipids. 1981 Jul;16(7):533–540. doi: 10.1007/BF02535052. [DOI] [PubMed] [Google Scholar]
  14. KENNEDY E. P., WEISS S. B. The function of cytidine coenzymes in the biosynthesis of phospholipides. J Biol Chem. 1956 Sep;222(1):193–214. [PubMed] [Google Scholar]
  15. Lombardi B., Pani P., Schlunk F. F., Shi-Hua C. Labelling of liver and plasma lecithins after injection of 1-2-14C-2-demethylaminoethanol and 14C-L-methionine-methyl to choline deficient rats. Lipids. 1969 Jan;4(1):67–75. doi: 10.1007/BF02531797. [DOI] [PubMed] [Google Scholar]
  16. Mato J. M., Alemany S. What is the function of phospholipid N-methylation? Biochem J. 1983 Jul 1;213(1):1–10. doi: 10.1042/bj2130001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Münzel P., Koschel K. Alteration in phospholipid methylation and impairment of signal transmission in persistently paramyxovirus-infected C6 rat glioma cells. Proc Natl Acad Sci U S A. 1982 Jun;79(12):3692–3696. doi: 10.1073/pnas.79.12.3692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pelech S. L., Pritchard P. H., Vance D. E. cAMP analogues inhibit phosphatidylcholine biosynthesis in cultured rat hepatocytes. J Biol Chem. 1981 Aug 25;256(16):8283–8286. [PubMed] [Google Scholar]
  19. Pritchard P. H., Chiang P. K., Cantoni G. L., Vance D. E. Inhibition of phosphatidylethanolamine N-methylation by 3-deazaadenosine stimulates the synthesis of phosphatidylcholine via the CDP-choline pathway. J Biol Chem. 1982 Jun 10;257(11):6362–6367. [PubMed] [Google Scholar]
  20. Pritchard P. H., Pelech S. L., Vance D. E. Analogues of cyclic AMP inhibit phosphatidylethanolamine N-methylation by cultured rat hepatocytes. Biochim Biophys Acta. 1981 Nov 23;666(2):301–306. doi: 10.1016/0005-2760(81)90122-3. [DOI] [PubMed] [Google Scholar]
  21. Schanche J. S., Ogreid D., Døskeland S. O., Refsnes M., Sand T. E., Ueland P. M., Christoffersen T. Evidence against a requirement for phospholipid methylation in adenylate cyclase activation by hormones. Methyltransferase inhibitors do not impair cyclic AMP accumulation induced by glucagon or beta-adrenergic agents in rat hepatocytes. FEBS Lett. 1982 Feb 22;138(2):167–172. doi: 10.1016/0014-5793(82)80433-x. [DOI] [PubMed] [Google Scholar]
  22. Schneider W. J., Vance D. E. Conversion of phosphatidylethanolamine to phosphatidylcholine in rat liver. Partial purification and characterization of the enzymatic activities. J Biol Chem. 1979 May 25;254(10):3886–3891. [PubMed] [Google Scholar]
  23. Schneider W. J., Vance D. E. Effect of choline deficiency on the enzymes that synthesize phosphatidylcholine and phosphatidylethanolamine in rat liver. Eur J Biochem. 1978 Apr;85(1):181–187. doi: 10.1111/j.1432-1033.1978.tb12226.x. [DOI] [PubMed] [Google Scholar]
  24. Sundler R., Akesson B. Regulation of phospholipid biosynthesis in isolated rat hepatocytes. Effect of different substrates. J Biol Chem. 1975 May 10;250(9):3359–3367. [PubMed] [Google Scholar]
  25. Thompson W., MacDonald G., Mookerjea S. Metabolism of phosphorylcholine and lecithin in normal and choline-deficient rats. Biochim Biophys Acta. 1969 Mar 4;176(2):306–315. doi: 10.1016/0005-2760(69)90188-x. [DOI] [PubMed] [Google Scholar]
  26. Vance D. E., de Kruijff B. The possible functional significance of phosphatidylethanolamine methylation. Nature. 1980 Nov 20;288(5788):277–279. doi: 10.1038/288277a0. [DOI] [PubMed] [Google Scholar]
  27. Wolfe B. B., Harden T. K., Molinoff P. B. beta-adrenergic receptors in rat liver: effects of adrenalectomy. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1343–1347. doi: 10.1073/pnas.73.4.1343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Zelenka P. S., Beebe D. C., Feagans D. E. Transmethylation of phosphatidylethanolamine: an initial event in embryonic chicken lens fiber cell differentiation. Science. 1982 Sep 24;217(4566):1265–1267. doi: 10.1126/science.7112130. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES