Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1985 Oct 1;231(1):47–51. doi: 10.1042/bj2310047

Proteinases from invasive larvae of the trematode parasite Schistosoma mansoni degrade connective-tissue and basement-membrane macromolecules.

J H McKerrow, P Jones, H Sage, S Pino-Heiss
PMCID: PMC1152701  PMID: 3904737

Abstract

Larvae of the human parasite Schistosoma mansoni, which invade the vascular system through the skin, secrete proteinases that degrade radioactively labeled extracellular matrices produced by smooth-muscle cells, dermal fibroblasts and endothelial cells. The proteinase purified from one larval form, the cercaria, degrades fibronectin and laminin and is a type-specific collagenase with activity against basement-membrane collagens IV and VIII, but not interstitial collagens I, III and V. The substrate specificity of this enzyme resembles that of the proteolytic enzymes which facilitate tissue invasion by inflammatory cells and tumour cells.

Full text

PDF
47

Images in this article

48Image
on p.48
49Fig. 2.
on p.49

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auriault C., Pierce R., Cesari I. M., Capron A. Neutral protease activities at different developmental stages of Schistosoma mansoni in mammalian hosts. Comp Biochem Physiol B. 1982;72(3):377–384. doi: 10.1016/0305-0491(82)90215-2. [DOI] [PubMed] [Google Scholar]
  2. Basch P. F. Cultivation of Schistosoma mansoni in vitro. I. Establishment of cultures from cercariae and development until pairing. J Parasitol. 1981 Apr;67(2):179–185. [PubMed] [Google Scholar]
  3. Bogenmann E., Jones P. A. Role of plasminogen in matrix breakdown by neoplastic cells. J Natl Cancer Inst. 1983 Dec;71(6):1177–1182. [PubMed] [Google Scholar]
  4. Bruce J. I., Pezzlo F., McCarty J. E., Yajima Y. Migration of Schistosoma mansoni through mouse tissue. Ultrastructure of host tissue and integument of migrating larva following cercarial penetration. Am J Trop Med Hyg. 1970 Nov;19(6):959–981. doi: 10.4269/ajtmh.1970.19.959. [DOI] [PubMed] [Google Scholar]
  5. Campbell D. L., Frappaolo P. J., Stirewalt M. A., Dresden M. H. Schistosoma mansoni: partial characterization of enzyme(s) secreted from the preacetabular glands of cercariae. Exp Parasitol. 1976 Aug;40(1):33–40. doi: 10.1016/0014-4894(76)90060-6. [DOI] [PubMed] [Google Scholar]
  6. Fessler L. I., Duncan K. G., Fessler J. H., Salo T., Tryggvason K. Characterization of the procollagen IV cleavage products produced by a specific tumor collagenase. J Biol Chem. 1984 Aug 10;259(15):9783–9789. [PubMed] [Google Scholar]
  7. Jones P. A., Scott-Burden T. Activated macrophages digest the extracellular matrix proteins produced by cultured cells. Biochem Biophys Res Commun. 1979 Jan 15;86(1):71–77. doi: 10.1016/0006-291x(79)90383-8. [DOI] [PubMed] [Google Scholar]
  8. Jones P. A., Scott-Burden T., Gevers W. Glycoprotein, elastin, and collagen secretion by rat smooth muscle cells. Proc Natl Acad Sci U S A. 1979 Jan;76(1):353–357. doi: 10.1073/pnas.76.1.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Keene W. E., Jeong K. H., McKerrow J. H., Werb Z. Degradation of extracellular matrix by larvae of Schistosoma mansoni. II. Degradation by newly transformed and developing schistosomula. Lab Invest. 1983 Aug;49(2):201–207. [PubMed] [Google Scholar]
  10. LEWERT R. M., LEE C. L. Studies on the passage of helminth larvae through host tissues. I. Histochemical studies on the extracellular changes caused by penetrating larvae. II. Enzymatic activity of larvae in vitro and in vivo. J Infect Dis. 1954 Jul-Aug;95(1):13–51. doi: 10.1093/infdis/95.1.13. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Landsperger W. J., Stirewalt M. A., Dresden M. H. Purification and properties of a proteolytic enzyme from the cercariae of the human trematode parasite Schistosoma mansoni. Biochem J. 1982 Jan 1;201(1):137–144. doi: 10.1042/bj2010137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laug W. E., DeClerck Y. A., Jones P. A. Degradation of the subendothelial matrix by tumor cells. Cancer Res. 1983 Apr;43(4):1827–1834. [PubMed] [Google Scholar]
  14. Liotta L. A., Lanzer W. L., Garbisa S. Identification of a type V collagenolytic enzyme. Biochem Biophys Res Commun. 1981 Jan 15;98(1):184–190. doi: 10.1016/0006-291x(81)91886-6. [DOI] [PubMed] [Google Scholar]
  15. Liotta L. A., Tryggvason K., Garbisa S., Hart I., Foltz C. M., Shafie S. Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature. 1980 Mar 6;284(5751):67–68. doi: 10.1038/284067a0. [DOI] [PubMed] [Google Scholar]
  16. Mainardi C. L., Seyer J. M., Kang A. H. Type-specific collagenolysis: a type V collagen-degrading enzyme from macrophages. Biochem Biophys Res Commun. 1980 Dec 16;97(3):1108–1115. doi: 10.1016/0006-291x(80)91490-4. [DOI] [PubMed] [Google Scholar]
  17. McKerrow J. H., Keene W. E., Jeong K. H., Werb Z. Degradation of extracellular matrix by larvae of Schistosoma mansoni. I. Degradation by cercariae as a model for initial parasite invasion of host. Lab Invest. 1983 Aug;49(2):195–200. [PubMed] [Google Scholar]
  18. McKerrow J. H., Pino-Heiss S., Lindquist R., Werb Z. Purification and characterization of an elastinolytic proteinase secreted by cercariae of Schistosoma mansoni. J Biol Chem. 1985 Mar 25;260(6):3703–3707. [PubMed] [Google Scholar]
  19. Ott U., Odermatt E., Engel J., Furthmayr H., Timpl R. Protease resistance and conformation of laminin. Eur J Biochem. 1982 Mar;123(1):63–72. doi: 10.1111/j.1432-1033.1982.tb06499.x. [DOI] [PubMed] [Google Scholar]
  20. Sage H., Bornstein P. Characterization of a novel collagen chain in human placenta and its relation to AB collagen. Biochemistry. 1979 Aug 21;18(17):3815–3822. doi: 10.1021/bi00584a027. [DOI] [PubMed] [Google Scholar]
  21. Sage H., Bornstein P. Preparation and characterization of procollagens and procollagen-collagen intermediates. Methods Enzymol. 1982;82(Pt A):96–127. doi: 10.1016/0076-6879(82)82061-2. [DOI] [PubMed] [Google Scholar]
  22. Sage H., Pritzl P., Bornstein P. A unique, pepsin-sensitive collagen synthesized by aortic endothelial cells in culture. Biochemistry. 1980 Dec 9;19(25):5747–5755. doi: 10.1021/bi00566a013. [DOI] [PubMed] [Google Scholar]
  23. Sage H., Trüeb B., Bornstein P. Biosynthetic and structural properties of endothelial cell type VIII collagen. J Biol Chem. 1983 Nov 10;258(21):13391–13401. [PubMed] [Google Scholar]
  24. Stirewalt M. A., Cousin C. E., Dorsey C. H. Schistosoma mansoni: stimulus and transformation of cercariae into schistosomules. Exp Parasitol. 1983 Dec;56(3):358–368. doi: 10.1016/0014-4894(83)90081-4. [DOI] [PubMed] [Google Scholar]
  25. Stirewalt M. A. Schistosoma mansoni: cercaria to schistosomule. Adv Parasitol. 1974;12:115–182. doi: 10.1016/s0065-308x(08)60388-7. [DOI] [PubMed] [Google Scholar]
  26. Tzeng S., McKerrow J. H., Fukuyama K., Jeong K., Epstein W. L. Degradation of purified skin keratin by a proteinase secreted from Schistosoma mansoni cercariae. J Parasitol. 1983 Oct;69(5):992–994. [PubMed] [Google Scholar]
  27. Werb Z., Banda M. J., Jones P. A. Degradation of connective tissue matrices by macrophages. I. Proteolysis of elastin, glycoproteins, and collagen by proteinases isolated from macrophages. J Exp Med. 1980 Nov 1;152(5):1340–1357. doi: 10.1084/jem.152.5.1340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. de Clerck Y. A., Jones P. A. The effect of ascorbic acid on the nature and production of collagen and elastin by rat smooth-muscle cells. Biochem J. 1980 Jan 15;186(1):217–225. doi: 10.1042/bj1860217. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES