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. 1985 Nov 15;232(1):133–137. doi: 10.1042/bj2320133

Effect of fructose 1-phosphate on the activation of liver glycogen synthase.

P Gergely, B Tóth, I Farkas, G Bot
PMCID: PMC1152849  PMID: 3936480

Abstract

The activation (dephosphorylation) of glycogen synthase and the inactivation (dephosphorylation) of phosphorylase in rat liver extracts on the administration of fructose were examined. The lag in the conversion of synthase b into a was cancelled, owing to the accumulation of fructose 1-phosphate. A decrease in the rate of dephosphorylation of phosphorylase a was also observed. The latency re-appeared in gel-filtered liver extracts. Similar latency was demonstrated in extracts from glucagon-treated rats. Addition of fructose 1-phosphate to the extract was able to abolish the latency, and the activation of glycogen synthase and the inactivation of phosphorylase occurred simultaneously. Fructose 1-phosphate increased the activity of glycogen synthase b measured in the presence of 0.2-0.4 mM-glucose 6-phosphate. According to kinetic investigations, fructose 1-phosphate increased the affinity of synthase b for its substrate, UDP-glucose. The accumulation of fructose 1-phosphate resulted in glycogen synthesis in the liver by inducing the enzymic activity of glycogen synthase b in the presence of glucose 6-phosphate in vivo and by promoting the activation of glycogen synthase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Burch H. B., Choi S., Dence C. N., Alvey T. R., Cole B. R., Lowry O. H. Metabolic effects of large fructose loads in different parts of the rat nephron. J Biol Chem. 1980 Sep 10;255(17):8239–8244. [PubMed] [Google Scholar]
  3. Burch H. B., Max P., Jr, Ghyu K., Lowry O. H. Metabolic intermediates in liver of rats given large amounts of fructose or dihydroxyacetone. Biochem Biophys Res Commun. 1969 Mar 10;34(5):619–626. doi: 10.1016/0006-291x(69)90783-9. [DOI] [PubMed] [Google Scholar]
  4. Camici M., Ahmad Z., DePaoli-Roach A. A., Roach P. J. Phosphorylation of rabbit liver glycogen synthase by multiple protein kinases. J Biol Chem. 1984 Feb 25;259(4):2466–2473. [PubMed] [Google Scholar]
  5. Ciudad C. J., Massagué J., Guinovart J. J. The inactivation of glycogen phosphorylase is not a prerequisite for the activation of liver glycogen synthase. FEBS Lett. 1979 Mar 15;99(2):321–324. doi: 10.1016/0014-5793(79)80982-5. [DOI] [PubMed] [Google Scholar]
  6. Ciudad C. J., Massagué J., Salavert A., Guinovart J. J. Synthesis of glycogen from fructose in the presence of elevated levels of glycogen phosphorylase a in rat hepatocytes. Mol Cell Biochem. 1980 Mar 20;30(1):33–38. doi: 10.1007/BF00215303. [DOI] [PubMed] [Google Scholar]
  7. Doperé F., Vanstapel F., Stalmans W. Glycogen-synthase phosphatase activity in rat liver. Two protein components and their requirement for the activation of different types of substrate. Eur J Biochem. 1980 Feb;104(1):137–146. doi: 10.1111/j.1432-1033.1980.tb04409.x. [DOI] [PubMed] [Google Scholar]
  8. Gilboe D. P., Nuttall F. Q. Direct glucose stimulation of glycogen synthase phosphatase activity in a liver glycogen particle preparation. Arch Biochem Biophys. 1984 Feb 1;228(2):587–591. doi: 10.1016/0003-9861(84)90026-2. [DOI] [PubMed] [Google Scholar]
  9. Iles R. A., Griffiths J. R., Stevens A. N., Gadian D. G., Porteous R. Effects of fructose on the energy metabolism and acid-base status of the perfused starved-rat liver. A 31phosphorus nuclear magnetic resonance study. Biochem J. 1980 Oct 15;192(1):191–202. doi: 10.1042/bj1920191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jakob A. Interactions of glucagon and fructose in the control of glycogenolysis in perfused rat liver. Mol Cell Endocrinol. 1976 Nov;6(1):47–58. doi: 10.1016/0303-7207(76)90043-5. [DOI] [PubMed] [Google Scholar]
  11. Kaufmann U., Froesch E. R. Inhibition of phosphorylase-a by fructose-1-phosphate, alpha-glycerophosphate and fructose-1,6-diphosphate: explanation for fructose-induced hypoglycaemia in hereditary fructose intolerance and fructose-1,6-diphosphatase deficiency. Eur J Clin Invest. 1973 Sep;3(5):407–413. doi: 10.1111/j.1365-2362.1973.tb02208.x. [DOI] [PubMed] [Google Scholar]
  12. Miller T. B., Jr Cyclic AMP-mediated activation of hepatic glycogenolysis by fructose. Biochim Biophys Acta. 1978 Apr 19;540(1):151–161. doi: 10.1016/0304-4165(78)90444-0. [DOI] [PubMed] [Google Scholar]
  13. Mvumbi L., Doperé F., Stalmans W. The inhibitory effect of phosphorylase a on the activation of glycogen synthase depends on the type of synthase phosphatase. Biochem J. 1983 May 15;212(2):407–416. doi: 10.1042/bj2120407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Niewoehner C. B., Gilboe D. P., Nuttall F. Q. Metabolic effects of oral glucose in the liver of fasted rats. Am J Physiol. 1984 Jan;246(1 Pt 1):E89–E94. doi: 10.1152/ajpendo.1984.246.1.E89. [DOI] [PubMed] [Google Scholar]
  15. Nuttall F. Q., Theen J. W., Niewoehner C., Gilboe D. P. Response of liver glycogen synthase and phosphorylase to in vivo glucose and glucose analogues. Am J Physiol. 1983 Nov;245(5 Pt 1):E521–E527. doi: 10.1152/ajpendo.1983.245.5.E521. [DOI] [PubMed] [Google Scholar]
  16. Regan J. J., Jr, Doorneweerd D. D., Gilboe D. P., Nuttall F. Q. Influence of fructose on the glycogen synthase and phosphorylase systems in rat liver. Metabolism. 1980 Oct;29(10):965–969. doi: 10.1016/0026-0495(80)90040-2. [DOI] [PubMed] [Google Scholar]
  17. Stalmans W., De Wulf H., Hue L., Hers H. G. The sequential inactivation of glycogen phosphorylase and activation of glycogen synthetase in liver after the administration of glucose to mice and rats. The mechanism of the hepatic threshold to glucose. Eur J Biochem. 1974 Jan 3;41(1):127–134. doi: 10.1111/j.1432-1033.1974.tb03252.x. [DOI] [PubMed] [Google Scholar]
  18. Stalmans W., Hers H. G. The stimulation of liver phosphorylase b by AMP, fluoride and sulfate. A technical note on the specific determination of the a and b forms of liver glycogen phosphorylase. Eur J Biochem. 1975 Jun;54(2):341–350. doi: 10.1111/j.1432-1033.1975.tb04144.x. [DOI] [PubMed] [Google Scholar]
  19. Stalmans W., de Wulf H., Hers H. G. The control of liver glycogen synthetase phosphatase by phosphorylase. Eur J Biochem. 1971 Feb;18(4):582–587. doi: 10.1111/j.1432-1033.1971.tb01279.x. [DOI] [PubMed] [Google Scholar]
  20. Tan A. W., Nuttall F. Q. Endogenous phosphates on liver glycogen synthase D and synthase I. Studies on the number and location. J Biol Chem. 1983 Aug 25;258(16):9624–9630. [PubMed] [Google Scholar]
  21. Tan A. W., Nuttall F. Q. Evidence for the non-identity of proteins having synthase phosphatase, phosphorylase phosphatase and histone phosphatase activity in rat liver. Biochim Biophys Acta. 1978 Jan 12;522(1):139–150. doi: 10.1016/0005-2744(78)90330-3. [DOI] [PubMed] [Google Scholar]
  22. Thurston J. H., Jones E. M., Hauhart R. E. Decrease and inhibition of liver glycogen phosphorylase after fructose. An experimental model for the study of hereditary fructose intolerance. Diabetes. 1974 Jul;23(7):597–604. doi: 10.2337/diab.23.7.597. [DOI] [PubMed] [Google Scholar]
  23. Van Den Berghe G., Hue L., Hers H. G. Effect of administration of the fructose on the glycogenolytic action of glucagon. An investigation of the pathogeny of hereditary fructose intolerance. Biochem J. 1973 Jun;134(2):637–645. doi: 10.1042/bj1340637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Van de Werve G., Hers H. G. Mechanism of activation of glycogen phosphorylase by fructose in the liver. Stimulation of phosphorylase kinase related to the consumption of adenosine triphosphate. Biochem J. 1979 Jan 15;178(1):119–126. doi: 10.1042/bj1780119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Van den Berghe G. Metabolic effects of fructose in the liver. Curr Top Cell Regul. 1978;13:97–135. doi: 10.1016/b978-0-12-152813-3.50008-2. [DOI] [PubMed] [Google Scholar]
  26. Whitton P. D., Hems D. A. Glycogen synthesis in the perfused liver of streptozotocin-diabetic rats. Biochem J. 1975 Aug;150(2):153–165. doi: 10.1042/bj1500153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Woods H. F., Eggleston L. V., Krebs H. A. The cause of hepatic accumulation of fructose 1-phosphate on fructose loading. Biochem J. 1970 Sep;119(3):501–510. doi: 10.1042/bj1190501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. de Wulf H., Hers H. G. The influence of inorganic phosphate, adenosine triphosphate and glucose 6-phosphate on the activity of liver glycogen synthetase. Eur J Biochem. 1968 Dec 5;6(4):545–551. doi: 10.1111/j.1432-1033.1968.tb00479.x. [DOI] [PubMed] [Google Scholar]

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