Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1986 Jan 15;233(2):613–616. doi: 10.1042/bj2330613

Structural analysis of the asparagine-linked oligosaccharides of human complement component C3.

S Hirani, J D Lambris, H J Müller-Eberhard
PMCID: PMC1153071  PMID: 3954758

Abstract

The asparagine-linked oligosaccharides of human C3 were characterized. The C3 oligosaccharides were released by endo-beta-N-acetylglucosaminidase H and were analysed by lectin affinity chromatography and h.p.l.c. The released oligosaccharides bound tightly to concanavalin A-Sepharose and were not retained by agarose-bound wheat-germ agglutinin, indicating that they were only of high-mannose type. Two major oligosaccharide structures were separated from both the alpha- and beta-chains of C3 by h.p.l.c. on Micropak AX-5, calibrated with high-mannose-type oligosaccharides of known structures. The oligosaccharide structures on the alpha-chain have the compositions (Man)9(GlcNAc)2-Asn and (Man)8 (GlcNAc)2-Asn, and those on the beta-chain have the compositions (Man)6(GlcNAc)2-Asn and (Man)5(GlcNAc)2-Asn.

Full text

PDF
614

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bokisch V. A., Dierich M. P., Mūller-Eberhard H. J. Third component of complement (C3): structural properties in relation to functions. Proc Natl Acad Sci U S A. 1975 Jun;72(6):1989–1993. doi: 10.1073/pnas.72.6.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chan A. C., Atkinson J. P. Oligosaccharide structure of human C4. J Immunol. 1985 Mar;134(3):1790–1798. [PubMed] [Google Scholar]
  3. Chapman A., Kornfeld R. Structure of the high mannose oligosaccharides of a human IgM myeloma protein. I. The major oligosaccharides of the two high mannose glycopeptides. J Biol Chem. 1979 Feb 10;254(3):816–823. [PubMed] [Google Scholar]
  4. Chiu F. J., Atkin C. L. Catabolites of the third component of complement in urines of hereditary nephritis patients. J Biol Chem. 1983 Jun 10;258(11):7200–7207. [PubMed] [Google Scholar]
  5. Endo Y., Yamashita K., Tachibana Y., Tojo S., Kobata A. Structures of the asparagine-linked sugar chains of human chorionic gonadotropin. J Biochem. 1979 Mar;85(3):669–679. [PubMed] [Google Scholar]
  6. Finne J., Krusius T. Preparation and fractionation of glycopeptides. Methods Enzymol. 1982;83:269–277. doi: 10.1016/0076-6879(82)83020-6. [DOI] [PubMed] [Google Scholar]
  7. Hirani S., Lambris J. D., Müller-Eberhard H. J. Localization of the conglutinin binding site on the third component of human complement. J Immunol. 1985 Feb;134(2):1105–1109. [PubMed] [Google Scholar]
  8. Hsieh P., Rosner M. R., Robbins P. W. Selective cleavage by endo-beta-N-acetylglucosaminidase H at individual glycosylation sites of Sindbis virion envelope glycoproteins. J Biol Chem. 1983 Feb 25;258(4):2555–2561. [PubMed] [Google Scholar]
  9. Kornfeld K., Reitman M. L., Kornfeld R. The carbohydrate-binding specificity of pea and lentil lectins. Fucose is an important determinant. J Biol Chem. 1981 Jul 10;256(13):6633–6640. [PubMed] [Google Scholar]
  10. Krusius T., Finne J., Rauvala H. The structural basis of the different affinities of two types of acidic N-glycosidic glycopeptides for concanavalin A--sepharose. FEBS Lett. 1976 Nov 15;72(1):117–120. doi: 10.1016/0014-5793(76)80911-8. [DOI] [PubMed] [Google Scholar]
  11. LEON M. A., YOKOHARI R. CONGLUTINATION: SPECIFIC INHIBITION BY CARBOHYDRATES. Science. 1964 Mar 20;143(3612):1327–1328. [PubMed] [Google Scholar]
  12. Lachmann P. J. Conglutinin and immunoconglutinins. Adv Immunol. 1967;6:479–527. doi: 10.1016/s0065-2776(08)60527-1. [DOI] [PubMed] [Google Scholar]
  13. Lambris J. D., Dobson N. J., Ross G. D. Release of endogenous C3b inactivator from lymphocytes in response to triggering membrane receptors for beta 1H globulin. J Exp Med. 1980 Dec 1;152(6):1625–1644. doi: 10.1084/jem.152.6.1625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Li E., Kornfeld S. Structural studies of the major high mannose oligosaccharide units from Chinese hamster ovary cell glycoproteins. J Biol Chem. 1979 Mar 10;254(5):1600–1605. [PubMed] [Google Scholar]
  15. Mellis S. J., Baenziger J. U. Separation of neutral oligosaccharides by high-performance liquid chromatography. Anal Biochem. 1981 Jul 1;114(2):276–280. doi: 10.1016/0003-2697(81)90480-2. [DOI] [PubMed] [Google Scholar]
  16. Morris K. M., Goldberger G., Colten H. R., Aden D. P., Knowles B. B. Biosynthesis and processing of a human precursor complement protein, pro-C3, in a hepatoma-derived cell line. Science. 1982 Jan 22;215(4531):399–400. doi: 10.1126/science.7199205. [DOI] [PubMed] [Google Scholar]
  17. Ogata S., Muramatsu T., Kobata A. Fractionation of glycopeptides by affinity column chromatography on concanavalin A-sepharose. J Biochem. 1975 Oct;78(4):687–696. doi: 10.1093/oxfordjournals.jbchem.a130956. [DOI] [PubMed] [Google Scholar]
  18. Tack B. D., Prahl J. W. Third component of human complement: purification from plasma and physicochemical characterization. Biochemistry. 1976 Oct 5;15(20):4513–4521. doi: 10.1021/bi00665a028. [DOI] [PubMed] [Google Scholar]
  19. Takahashi T., Schmidt P. G., Tang J. Oligosaccharide units of lysosomal cathepsin D from porcine spleen. Amino acid sequence and carbohydrate structure of the glycopeptides. J Biol Chem. 1983 Mar 10;258(5):2819–2830. [PubMed] [Google Scholar]
  20. Taylor J. C., Crawford I. P., Hugli T. E. Limited degradation of the third component (C3) of human complement by human leukocyte elastase (HLE): partial characterization of C3 fragments. Biochemistry. 1977 Jul 26;16(15):3390–3396. doi: 10.1021/bi00634a016. [DOI] [PubMed] [Google Scholar]
  21. Tomana M., Niemann M., Garner C., Volanakis J. E. Carbohydrate composition of the second, third and fifth components and factors B and D of human complement. Mol Immunol. 1985 Feb;22(2):107–111. doi: 10.1016/s0161-5890(85)80004-3. [DOI] [PubMed] [Google Scholar]
  22. Varki A., Kornfeld S. The spectrum of anionic oligosaccharides released by endo-beta-N-acetylglucosaminidase H from glycoproteins. Structural studies and interactions with the phosphomannosyl receptor. J Biol Chem. 1983 Mar 10;258(5):2808–2818. [PubMed] [Google Scholar]
  23. Yamamoto K., Tsuji T., Matsumoto I., Osawa T. Structural requirements for the binding of oligosaccharides and glycopeptides to immobilized wheat germ agglutinin. Biochemistry. 1981 Sep 29;20(20):5894–5899. doi: 10.1021/bi00523a037. [DOI] [PubMed] [Google Scholar]
  24. de Bruijn M. H., Fey G. H. Human complement component C3: cDNA coding sequence and derived primary structure. Proc Natl Acad Sci U S A. 1985 Feb;82(3):708–712. doi: 10.1073/pnas.82.3.708. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES