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. 1984 Mar 15;218(3):893–897. doi: 10.1042/bj2180893

Evidence for two forms of ligandin (YaYa dimers of glutathione S-transferase) in rat liver and kidney.

D Sheehan, T J Mantle
PMCID: PMC1153420  PMID: 6721840

Abstract

CM-cellulose chromatography of rat liver and kidney cytosol at pH6 reveals the presence of a second Ya-subunit dimer of glutathione S-transferase (GST-F) in addition to the recently described GST-YaYa (GST-L; our nomenclature) [Hayes & Clarkson (1982) Biochem. J. 207, 459-470]. The two forms are structurally similar (by the criteria of CNBr- and Staphylococcus-V8-proteinase-cleavage peptide maps), and both are sensitive to inhibition by haemin. However, their kinetic parameters with 1-chloro-2,4-dinitrobenzene are quite distinct, and they show differential inducibility by phenobarbitone. These results suggest a similar heterogeneity in Ya-subunits to that previously described for Yb-subunits of glutathione S-transferase and indicate that significant gene duplication may have occurred in these multifunctional intracellular binding proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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