Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1984 May 1;219(3):781–784. doi: 10.1042/bj2190781

Phospholipid substrate-specificity of the L-serine base-exchange enzyme in rat liver microsomal fraction.

K S Bjerve
PMCID: PMC1153544  PMID: 6430274

Abstract

The specificity of the L-serine base-exchange enzyme towards the fatty acid composition of the phospholipid substrate was investigated with a rat liver microsomal fraction. The relative rates of L-serine incorporation into saturated-hexaenoic, saturated-pentaenoic, saturated-tetraenoic, saturated-trienoic, dienoic-dienoic, monoenoic-dienoic, saturated-dienoic and saturated-monoenoic + saturated-saturated phosphatidylserine molecular species were 42, 5, 23, 4, 5, 4, 5 and 11% respectively. This is similar to, but not identical with, the relative mass abundance of these molecular species in total liver cell phosphatidylserines. The results indicate that the substrate-specificity of the L-serine base-exchange enzyme can at least in part explain the observed fatty acid composition of rat liver phosphatidylserines.

Full text

PDF
781

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arvidson G. A. Structural and metabolic heterogeneity of rat liver glycerophosphatides. Eur J Biochem. 1968 May;4(4):478–486. doi: 10.1111/j.1432-1033.1968.tb00237.x. [DOI] [PubMed] [Google Scholar]
  2. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  3. Barańska J. Biosynthesis and transport of phosphatidylserine in the cell. Adv Lipid Res. 1982;19:163–184. doi: 10.1016/b978-0-12-024919-0.50011-4. [DOI] [PubMed] [Google Scholar]
  4. Barańska J. Phosphatidylserine biosynthesis in mitochondria from Ehrlich ascites tumor cells. Biochim Biophys Acta. 1980 Aug 11;619(2):258–266. doi: 10.1016/0005-2760(80)90074-0. [DOI] [PubMed] [Google Scholar]
  5. Bjerve K. S. Chromatographic resolution of molecular species of phosphatidylserines and phosphatidylethanolamines as their N-trifluoroacetyl-O-methyl and N-acetyl-O-methyl derivatives. J Chromatogr. 1982 Oct 8;232(1):39–46. doi: 10.1016/s0378-4347(00)86005-3. [DOI] [PubMed] [Google Scholar]
  6. Bjerve K. S. The Ca 2+ -dependent biosynthesis of lecithin, phosphatidyl-ethanolamine and phosphatidylserine in rat liver subcellular particles. Biochim Biophys Acta. 1973 Mar 8;296(3):549–562. [PubMed] [Google Scholar]
  7. Bjerve K. S. The Ca(2+) stimulated incorporation of choline into microsomal lecithin subspecies in vitro. FEBS Lett. 1971 Sep 15;17(1):14–16. doi: 10.1016/0014-5793(71)80551-3. [DOI] [PubMed] [Google Scholar]
  8. Bjerve K. S. The phospholipid substrates in the Ca2+ -stimulated incorporation of nitrogen bases into microsomal phospholipids. Biochim Biophys Acta. 1973 Jun 21;306(3):396–402. doi: 10.1016/0005-2760(73)90178-1. [DOI] [PubMed] [Google Scholar]
  9. Bygrave F. L., Bücher T. The energy-dependent incorporation of serine into phospholipids of isolated rat liver mitochondria. Eur J Biochem. 1968 Nov;6(2):256–263. doi: 10.1111/j.1432-1033.1968.tb00445.x. [DOI] [PubMed] [Google Scholar]
  10. DILS R. R., HUBSCHER G. Metabolism of phospholipids. III. The effect of calcium ions on the incorporation of labelled choline into rat-liver microsomes. Biochim Biophys Acta. 1961 Jan 29;46:505–513. doi: 10.1016/0006-3002(61)90581-9. [DOI] [PubMed] [Google Scholar]
  11. HUBSCHER G., DILS R. R., POVER W. F. Studies on the biosynthesis of phosphatidyl serine. Biochim Biophys Acta. 1959 Dec;36:518–528. doi: 10.1016/0006-3002(59)90194-5. [DOI] [PubMed] [Google Scholar]
  12. Holub B. J. The biosynthesis of phosphatidylserines by acylation of 1-acyl-sn-glycero-3-phosphoserine in rat liver. Biochim Biophys Acta. 1980 May 28;618(2):255–262. doi: 10.1016/0005-2760(80)90031-4. [DOI] [PubMed] [Google Scholar]
  13. Kanfer J. N. The base exchange enzymes and phospholipase D of mammalian tissue. Can J Biochem. 1980 Dec;58(12):1370–1380. doi: 10.1139/o80-186. [DOI] [PubMed] [Google Scholar]
  14. LANDS W. E., MERKL I. Metabolism of glycerolipids. III. Reactivity of various acyl esters of coenzyme A with alpha'-acylglycerophosphorylcholine, and positional specificities in lecithin synthesis. J Biol Chem. 1963 Mar;238:898–904. [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Miura T., Kanfer J. Studies on base-exchange reactions of phospholipids in rat brain. Heterogeneity of base-exchange enzymes. Arch Biochem Biophys. 1976 Aug;175(2):654–660. doi: 10.1016/0003-9861(76)90556-7. [DOI] [PubMed] [Google Scholar]
  17. Porcellati G., Arienti G., Pirotta M., Giorgini D. Base-exchange reactions for the synthesis of phospholipids in nervous tissue: the incorporation of serine and ethanolamine into the phospholipids of isolated brain microsomes. J Neurochem. 1971 Aug;18(8):1395–1417. doi: 10.1111/j.1471-4159.1971.tb00004.x. [DOI] [PubMed] [Google Scholar]
  18. Pullarkat R. K., Sbaschnig-Agler M., Reha H. Biosynthesis of phosphatidylserine in rat brain microsomes. Biochim Biophys Acta. 1981 Apr 23;664(1):117–123. doi: 10.1016/0005-2760(81)90034-5. [DOI] [PubMed] [Google Scholar]
  19. Taki T., Kanfer J. N. A phospholipid serine base exchange enzyme. Biochim Biophys Acta. 1978 Mar 30;528(3):309–317. doi: 10.1016/0005-2760(78)90020-6. [DOI] [PubMed] [Google Scholar]
  20. Webb R. A., Mettrick D. F. Quantitative liquid scintillation radioassay of phospholipids from thin-layer chromatograms. J Chromatogr. 1972 Apr 26;67(1):75–78. doi: 10.1016/s0021-9673(01)97149-3. [DOI] [PubMed] [Google Scholar]
  21. Yeung S. K., Kuksis A., Marai L., Myher J. J. Resolution of molecular species of intact serine and ethanolamine phosphatides by argentation chromatography of their trifluoroacetamides. Lipids. 1977 Jul;12(7):529–537. doi: 10.1007/BF02533377. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES