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. 1984 May 1;219(3):953–957. doi: 10.1042/bj2190953

Changes in prostaglandin release associated with inhibition of muscle protein synthesis by dexamethasone.

P J Reeds, R M Palmer
PMCID: PMC1153567  PMID: 6588968

Abstract

Forelimb digit extensor muscles from fed rabbits were incubated in the absence or presence of dexamethasone (100 nM). The presence of dexamethasone decreased the rates of protein synthesis, prostaglandin F2 alpha and prostaglandin E2 release after a time lag of 2.5-3 h. Although intermittent stretching stimulated both protein synthesis and prostaglandin release in the presence of dexamethasone, the absolute activities of both processes were lower in the presence of the steroid than in its absence. It is suggested that the inhibitory action of dexamethasone on muscle protein synthesis in vitro results from its effect on the activity of plasma-membrane phospholipase A2.

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Selected References

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  1. BARRETT A. M., STOCKHAM M. A. The effect of housing conditions and simple experimental procedures upon the corticosterone level in the plasma of rats. J Endocrinol. 1963 Mar;26:97–105. doi: 10.1677/joe.0.0260097. [DOI] [PubMed] [Google Scholar]
  2. Baracos V., Rodemann H. P., Dinarello C. A., Goldberg A. L. Stimulation of muscle protein degradation and prostaglandin E2 release by leukocytic pyrogen (interleukin-1). A mechanism for the increased degradation of muscle proteins during fever. N Engl J Med. 1983 Mar 10;308(10):553–558. doi: 10.1056/NEJM198303103081002. [DOI] [PubMed] [Google Scholar]
  3. Bellamy D., Leonard R. A., Dulieu K., Stevenson A. Starvation metabolism and plasma corticosterone with reference to the actions of metopirone and propylthiouracil. Gen Comp Endocrinol. 1968 Feb;10(1):119–125. doi: 10.1016/0016-6480(68)90017-8. [DOI] [PubMed] [Google Scholar]
  4. Blackwell G. J., Carnuccio R., Di Rosa M., Flower R. J., Parente L., Persico P. Macrocortin: a polypeptide causing the anti-phospholipase effect of glucocorticoids. Nature. 1980 Sep 11;287(5778):147–149. doi: 10.1038/287147a0. [DOI] [PubMed] [Google Scholar]
  5. Bullock G. R., Carter E. E., Elliott P., Peters R. F., Simpson P., White A. M. Relative changes in the function of muscle ribosomes and mitochondria during the early phase of steroid-induced catabolism. Biochem J. 1972 May;127(5):881–892. doi: 10.1042/bj1270881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bullock G., White A. M., Worthington J. The effects of catabolic and anabolic steroids on amino acid incorporation by skeletal-muscle ribosomes. Biochem J. 1968 Jul;108(3):417–425. doi: 10.1042/bj1080417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chandrabose K. A., Lapetina E. G., Schmitges C. J., Siegel M. I., Cuatrecasas P. Action of corticosteroids in regulation of prostaglandin biosynthesis in cultured fibroblasts. Proc Natl Acad Sci U S A. 1978 Jan;75(1):214–217. doi: 10.1073/pnas.75.1.214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Danon A., Assouline G. Inhibition of prostaglandin biosynthesis by corticosteroids requires RNA and protein synthesis. Nature. 1978 Jun 15;273(5663):552–554. doi: 10.1038/273552a0. [DOI] [PubMed] [Google Scholar]
  9. Fain J. N., Kovacev V. P., Scow R. O. Effect of growth hormone and dexamethasone on lipolysis and metabolism in isolated fat cells of the rat. J Biol Chem. 1965 Sep;240(9):3522–3529. [PubMed] [Google Scholar]
  10. Flaim K. E., Copenhaver M. E., Jefferson L. S. Effects of diabetes on protein synthesis in fast- and slow-twitch rat skeletal muscle. Am J Physiol. 1980 Jul;239(1):E88–E95. doi: 10.1152/ajpendo.1980.239.1.E88. [DOI] [PubMed] [Google Scholar]
  11. Flower R. J., Blackwell G. J. Anti-inflammatory steroids induce biosynthesis of a phospholipase A2 inhibitor which prevents prostaglandin generation. Nature. 1979 Mar 29;278(5703):456–459. doi: 10.1038/278456a0. [DOI] [PubMed] [Google Scholar]
  12. Garlick P. J., Fern M., Preedy V. R. The effect of insulin infusion and food intake on muscle protein synthesis in postabsorptive rats. Biochem J. 1983 Mar 15;210(3):669–676. doi: 10.1042/bj2100669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Goldberg A. L., Goodman H. M. Relationship between cortisone and muscle work in determining muscle size. J Physiol. 1969 Feb;200(3):667–675. doi: 10.1113/jphysiol.1969.sp008715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hassid A. Transport-active renal tubular epithelial cells (MDCK and LLC-PK1) in culture. Prostaglandin biosynthesis and its regulation by peptide hormones and ionophore. Prostaglandins. 1981 Jun;21(6):985–1001. doi: 10.1016/0090-6980(81)90166-0. [DOI] [PubMed] [Google Scholar]
  15. Hirata F., Schiffmann E., Venkatasubramanian K., Salomon D., Axelrod J. A phospholipase A2 inhibitory protein in rabbit neutrophils induced by glucocorticoids. Proc Natl Acad Sci U S A. 1980 May;77(5):2533–2536. doi: 10.1073/pnas.77.5.2533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hong S. L., Levine L. Inhibition of arachidonic acid release from cells as the biochemical action of anti-inflammatory corticosteroids. Proc Natl Acad Sci U S A. 1976 May;73(5):1730–1734. doi: 10.1073/pnas.73.5.1730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Irvine R. F. How is the level of free arachidonic acid controlled in mammalian cells? Biochem J. 1982 Apr 15;204(1):3–16. doi: 10.1042/bj2040003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Jimenez de Asua L., O'Farrell M., Bennett D., Clingan D., Rugland P. Interaction of two hormones and their effect on observed rate of initiation of DNA synthesis in 3T3 cells. Nature. 1977 Jan 13;265(5590):151–153. doi: 10.1038/265151a0. [DOI] [PubMed] [Google Scholar]
  19. Kantrowitz F., Robinson D. R., McGuire M. B., Levine L. Corticosteroids inhibit prostaglandin production by rheumatiod synovia. Nature. 1975 Dec 25;258(5537):737–739. doi: 10.1038/258737a0. [DOI] [PubMed] [Google Scholar]
  20. Kostyo J. L., Redmond A. F. Role of protein synthesis in the inhibitory action of adrenal steroid hormones on amino acid transport by muscle. Endocrinology. 1966 Sep;79(3):531–540. doi: 10.1210/endo-79-3-531. [DOI] [PubMed] [Google Scholar]
  21. Laurent G. J., Sparrow M. P., Millward D. J. Turnover of muscle protein in the fowl. Changes in rates of protein synthesis and breakdown during hypertrophy of the anterior and posterior latissimus dorsi muscles. Biochem J. 1978 Nov 15;176(2):407–417. doi: 10.1042/bj1760407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. McGrath J. A., Goldspink D. F. Glucocorticoid action on protein synthesis and protein breakdown in isolated skeletal muscles. Biochem J. 1982 Sep 15;206(3):641–645. doi: 10.1042/bj2060641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Millward D. J., Garlick P. J., Nnanyelugo D. O., Waterlow J. C. The relative importance of muscle protein synthesis and breakdown in the regulation of muscle mass. Biochem J. 1976 Apr 15;156(1):185–188. doi: 10.1042/bj1560185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Millward D. J., Garlick P. J., Stewart R. J., Nnanyelugo D. O., Waterlow J. C. Skeletal-muscle growth and protein turnover. Biochem J. 1975 Aug;150(2):235–243. doi: 10.1042/bj1500235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Millward D. J., Odedra B., Bates P. C. The role of insulin, corticosterone and other factors in the acute recovery of muscle protein synthesis on refeeding food-deprived rats. Biochem J. 1983 Dec 15;216(3):583–587. doi: 10.1042/bj2160583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Nijkamp F. P., Flower R. J., Moncada S., Vane J. R. Partial purification of rabbit aorta contracting substance-releasing factor and inhibition of its activity by anti-inflammatory steroids. Nature. 1976 Oct 7;263(5577):479–482. doi: 10.1038/263479a0. [DOI] [PubMed] [Google Scholar]
  27. Odedra B. R., Bates P. C., Millward D. J. Time course of the effect of catabolic doses of corticosterone on protein turnover in rat skeletal muscle and liver. Biochem J. 1983 Aug 15;214(2):617–627. doi: 10.1042/bj2140617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Odedra B. R., Millward D. J. Effect of corticosterone treatment on muscle protein turnover in adrenalectomized rats and diabetic rats maintained on insulin. Biochem J. 1982 Jun 15;204(3):663–672. doi: 10.1042/bj2040663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Palmer R. M., Reeds P. J., Atkinson T., Smith R. H. The influence of changes in tension on protein synthesis and prostaglandin release in isolated rabbit muscles. Biochem J. 1983 Sep 15;214(3):1011–1014. doi: 10.1042/bj2141011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Palmer R. M., Reeds P. J., Lobley G. E., Smith R. H. The effect of intermittent changes in tension on protein and collagen synthesis in isolated rabbit muscles. Biochem J. 1981 Sep 15;198(3):491–498. doi: 10.1042/bj1980491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Rannels S. R., Jefferson L. S. Effects of glucocorticoids on muscle protein turnover in perfused rat hemicorpus. Am J Physiol. 1980 Jun;238(6):E564–E572. doi: 10.1152/ajpendo.1980.238.6.E564. [DOI] [PubMed] [Google Scholar]
  32. Reeds P. J., Haggarty P., Wahle K. W., Fletcher J. M. Tissue and whole-body protein synthesis in immature Zucker rats and their relationship to protein deposition. Biochem J. 1982 May 15;204(2):393–398. doi: 10.1042/bj2040393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Reeds P. J., Palmer R. M., Smith R. H. Protein and collagen synthesis in rat diaphragm muscle incubated in vitro: the effect of alterations in tension produced by electrical or mechanical means. Int J Biochem. 1980;11(1):7–14. doi: 10.1016/0020-711x(80)90274-8. [DOI] [PubMed] [Google Scholar]
  34. Reeds P. J., Palmer R. M. The possible involvement of prostaglandin F2 alpha in the stimulation of muscle protein synthesis by insulin. Biochem Biophys Res Commun. 1983 Nov 15;116(3):1084–1090. doi: 10.1016/s0006-291x(83)80253-8. [DOI] [PubMed] [Google Scholar]
  35. Rodemann H. P., Goldberg A. L. Arachidonic acid, prostaglandin E2 and F2 alpha influence rates of protein turnover in skeletal and cardiac muscle. J Biol Chem. 1982 Feb 25;257(4):1632–1638. [PubMed] [Google Scholar]
  36. Rodemann H. P., Waxman L., Goldberg A. L. The stimulation of protein degradation in muscle by Ca2+ is mediated by prostaglandin E2 and does not require the calcium-activated protease. J Biol Chem. 1982 Aug 10;257(15):8716–8723. [PubMed] [Google Scholar]
  37. Rothhut B., Cloix J. F., Russo-Marie F. Dexamethasone induces the synthesis of "renocortins," two antiphospholipase proteins in rat renomedullary interstitial cells in culture. Adv Prostaglandin Thromboxane Leukot Res. 1983;12:51–56. [PubMed] [Google Scholar]
  38. Smith R. H., Palmer R. M., Reeds P. J. Protein synthesis in isolated rabbit forelimb muscles. The possible role of metabolites of arachidonic acid in the response to intermittent stretching. Biochem J. 1983 Jul 15;214(1):153–161. doi: 10.1042/bj2140153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Stirewalt W. S., Low R. B. Effects of insulin in vitro on protein turnover in rat epitrochlearis muscle. Biochem J. 1983 Feb 15;210(2):323–330. doi: 10.1042/bj2100323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Tashjian A. H., Jr, Voelkel E. F., McDonough J., Levine L. Hydrocortisone inhibits prostaglandin production by mouse fibrosarcoma cells. Nature. 1975 Dec 25;258(5537):739–741. doi: 10.1038/258739a0. [DOI] [PubMed] [Google Scholar]
  41. Thompson E. B., Lippman M. E. Mechanism of action of glucocorticoids. Metabolism. 1974 Feb;23(2):159–202. doi: 10.1016/0026-0495(74)90113-9. [DOI] [PubMed] [Google Scholar]
  42. Tomas F. M., Munro H. N., Young V. R. Effect of glucocorticoid administration on the rate of muscle protein breakdown in vivo in rats, as measured by urinary excretion of N tau-methylhistidine. Biochem J. 1979 Jan 15;178(1):139–146. doi: 10.1042/bj1780139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Turner L. V., Garlick P. J. The effect of unilateral phrenicectomy on the rate of protein synthesis in rat diaphragm in vivo. Biochim Biophys Acta. 1974 Apr 27;349(1):109–113. doi: 10.1016/0005-2787(74)90013-6. [DOI] [PubMed] [Google Scholar]
  44. Vernon B. G., Buttery P. J. Protein turnover in rats treated with trienbolone acetate. Br J Nutr. 1976 Nov;36(3):575–579. doi: 10.1079/bjn19760112. [DOI] [PubMed] [Google Scholar]

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