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. 1984 Jun 15;220(3):767–772. doi: 10.1042/bj2200767

The kinetics of ammonia release during the catalytic cycle of pig plasma amine oxidase.

F X Rius, P F Knowles, G Pettersson
PMCID: PMC1153694  PMID: 6466302

Abstract

The time course of NH3 release during the catalytic cycle of pig plasma amine oxidase was followed by using the quenched-flow technique in conjunction with a sensitive assay for NH3. These studies were made under both air and O2-saturating conditions. The results establish unequivocally that NH3 is released in the step whereby a reduced enzyme species is re-oxidized by molecular O2 rather than in the step leading to the reduced enzyme. It is concluded that the catalytic cycle of the enzyme conforms to an aminotransferase mechanism rather than one in which an imine is an intermediate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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