Abstract
A sensitive and specific radioimmunoassay for the 148 kDa cartilage protein (previously referred to as 'cartilage matrix protein') was developed. The protein is insoluble in conventional buffers and was therefore dissolved in sodium dodecyl sulphate. Excess sodium dodecyl sulphate was bound in mixed micelles with Triton X-100 before assay. Antibodies raised against the 148 kDa cartilage protein did not react with other cartilage macromolecules. Neither did they react with guanidinium chloride extracts of a number of non-cartilaginous tissues. The protein, then, is unique for cartilage. It was demonstrated in tracheal, nasal-septum, xiphisternal, auricular and epiphysial cartilage. Surprisingly, the protein was detected neither in extracts of articular cartilage nor in extracts of the anulus fibrosus or the nucleus pulposus of the intervertebral disc.
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- Caterson B., Baker J. R., Levitt D., Paslay J. W. Radioimmunoassay of the link proteins associated with bovine nasal cartilage proteoglycan. J Biol Chem. 1979 Oct 10;254(19):9369–9372. [PubMed] [Google Scholar]
- Converse C. A., Papermaster D. S. Membrane protein analysis by two-dimensional immunoelectrophoresis. Science. 1975 Aug 8;189(4201):469–472. doi: 10.1126/science.1154021. [DOI] [PubMed] [Google Scholar]
- GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller E. J. Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin. Biochemistry. 1972 Dec 19;11(26):4903–4909. doi: 10.1021/bi00776a005. [DOI] [PubMed] [Google Scholar]
- Paulsson M., Heinegård D. Matrix proteins bound to associatively prepared proteoglycans from bovine cartilage. Biochem J. 1979 Dec 1;183(3):539–545. doi: 10.1042/bj1830539. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paulsson M., Heinegård D. Purification and structural characterization of a cartilage matrix protein. Biochem J. 1981 Aug 1;197(2):367–375. doi: 10.1042/bj1970367. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rodbard D., Bridson W., Rayford P. L. Rapid calculation of radioimmunoassay results. J Lab Clin Med. 1969 Nov;74(5):770–781. [PubMed] [Google Scholar]
- Wieslander J., Heinegárd D. Immunochemical analysis of cartilage proteoglycans. Radioimmunoassay of the molecules and the substructures. Biochem J. 1980 Jun 1;187(3):687–694. doi: 10.1042/bj1870687. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wieslander J., Heinegård D. Immunochemical analysis of cartilage proteoglycans. Antigenic determinants of substructures. Biochem J. 1979 Apr 1;179(1):35–45. doi: 10.1042/bj1790035. [DOI] [PMC free article] [PubMed] [Google Scholar]