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. 1982 Dec 1;207(3):529–533. doi: 10.1042/bj2070529

The determination by radiochemical assay of argininosuccinase produced in an Escherichia coli system in vitro.

M J Zidwick, P Rogers
PMCID: PMC1153893  PMID: 6762207

Abstract

A highly sensitive radiochemical assay used to measure the synthesis and regulation of the product of the argH gene, argininosuccinase, in an Escherichia coli system in vitro is described. With L-[guanidino-14C]argininosuccinic acid as a substrate, and in the presence of excess arginase and urease, 14CO2 is collected in a simply designed micro-vessel. With this method less than 1 nmol of product can be measured in the presence of various concentrations of L-arginine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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