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. 1982 Dec 1;207(3):613–616. doi: 10.1042/bj2070613

Comparative studies on oestrogen-induced rat uterus peroxidase and rat eosinophil peroxidase

Ragner L Olsen 1, Clive Little 1
PMCID: PMC1153907  PMID: 6299274

Abstract

Rat eosinophil peroxidase and rat uterine peroxidase II showed similar electrophoretic mobilities, molecular weights, specific activities and spectral properties and could be purified by essentially identical techniques. Antibodies raised against the uterine enzyme strongly inhibited the eosinophil enzyme. It is suggested that rat eosinophil peroxidase and rat uterine peroxidase II may well be one and the same enzyme.

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Selected References

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  1. Anderson W. A., Kang Y. H., DeSombre E. R. Endogenous peroxidase: specific marker enzyme for tissues displaying growth dependency on estrogen. J Cell Biol. 1975 Mar;64(3):668–681. doi: 10.1083/jcb.64.3.668. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brökelmann J., Fawcett D. W. The localization of endogenous peroxidase in the rat uterus and its induction by estradiol. Biol Reprod. 1969 Apr;1(1):59–71. doi: 10.1095/biolreprod1.1.59. [DOI] [PubMed] [Google Scholar]
  3. Brökelmann J. Peroxidase-associated binding of estradiol by the rat uterus. J Histochem Cytochem. 1969 Jun;17(6):394–397. doi: 10.1177/17.6.394. [DOI] [PubMed] [Google Scholar]
  4. Collings J. R., Savage N. Peroxidase as a marker for oestrogen dependence in human breast cancer. Br J Cancer. 1979 Sep;40(3):500–503. doi: 10.1038/bjc.1979.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  6. Duffy M. J., O'Connell M. Estrogens, estradiol receptors and peroxidase activity in human mammary carcinomas. Eur J Cancer. 1981 Jun;17(6):711–714. doi: 10.1016/0014-2964(81)90276-0. [DOI] [PubMed] [Google Scholar]
  7. Holt J. A., Lyttle C. R., Lorincz M. A., Stern S. D., Press M. F., Herbst A. L. Estrogen receptor and peroxidase activity in epithelial ovarian carcinomas. J Natl Cancer Inst. 1981 Aug;67(2):307–318. [PubMed] [Google Scholar]
  8. KLEBANOFF S. J. INACTIVATION OF ESTROGEN BY RAT UTERINE PREPARATIONS. Endocrinology. 1965 Feb;76:301–311. doi: 10.1210/endo-76-2-301. [DOI] [PubMed] [Google Scholar]
  9. Keeping H. S., Kimura S., Løvsted J., Jellinck P. H. Further purification and properties of rat uterine peroxidase. Can J Biochem. 1981 Nov-Dec;59(11-12):916–920. doi: 10.1139/o81-129. [DOI] [PubMed] [Google Scholar]
  10. King W. J., Allen T. C., DeSombre E. R. Localization of uterine peroxidase activity in estrogen-treated rats. Biol Reprod. 1981 Nov;25(4):859–870. doi: 10.1095/biolreprod25.4.859. [DOI] [PubMed] [Google Scholar]
  11. LUCAS F. V., NEUFELD H. A., UTTERBACK J. G., MARTIN A. P., STOTZ E. The effect of estrogen on the production of a peroxidase in the rat uterus. J Biol Chem. 1955 Jun;214(2):775–780. [PubMed] [Google Scholar]
  12. Levy J., Liel Y., Glick S. M. Peroxidase activity as a marker for estrogenicity: studies in uterus and mammary tumors. Isr J Med Sci. 1981 Sep-Oct;17(9-10):970–975. [PubMed] [Google Scholar]
  13. Lyttle C. R., Jellinck P. H. Metabolism of (4- 14 C)oestradiol by oestrogen-induced uterine peroxidase. Biochem J. 1972 Apr;127(3):481–487. doi: 10.1042/bj1270481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McNabb T., Jellinck P. H. Origin of oestrogen-induced uterine peroxidase. J Endocrinol. 1974 Aug;62(2):415–416. doi: 10.1677/joe.0.0620415. [DOI] [PubMed] [Google Scholar]
  15. McNabb T., Jellinck P. H. Purification and properties of oestrogen-induced uterine peroxidase. Biochem J. 1975 Nov;151(2):275–279. doi: 10.1042/bj1510275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Olsen R. L., Flatmark T., Little C. Spectral properties of the oestrogen-induced rat uterus peroxidase II and some of its derivatives. Biochem J. 1982 Jan 1;201(1):91–94. doi: 10.1042/bj2010091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Olsen R. L., Little C. The peroxidase activity of rat uterus. Eur J Biochem. 1979 Nov;101(2):333–339. doi: 10.1111/j.1432-1033.1979.tb19725.x. [DOI] [PubMed] [Google Scholar]
  18. Penney G. C., Scott K. M., Hawkins R. A. Endogenous peroxidase: an alternative to oestrogen receptor in the management of breast cancer? Br J Cancer. 1980 Apr;41(4):648–651. doi: 10.1038/bjc.1980.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. RYTOMAA T., TEIR H. Relationship between tissue eosinophils and peroxidase activity. Nature. 1961 Oct 21;192:271–272. doi: 10.1038/192271b0. [DOI] [PubMed] [Google Scholar]
  20. Schaffner W., Weissmann C. A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal Biochem. 1973 Dec;56(2):502–514. doi: 10.1016/0003-2697(73)90217-0. [DOI] [PubMed] [Google Scholar]
  21. Stevenson G. T., Dorrington K. J. The recombination of dimers of immunoglobulin peptide chains. Biochem J. 1970 Aug;118(5):703–712. doi: 10.1042/bj1180703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Strum J. M., Becci P. J. Analysis of mammary tumors for cytochemical evidence of endogenous mammary peroxidase. Virchows Arch B Cell Pathol Incl Mol Pathol. 1979 Oct;31(2):135–142. doi: 10.1007/BF02889931. [DOI] [PubMed] [Google Scholar]

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