Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1982 Dec 1;207(3):641–644. doi: 10.1042/bj2070641

A correlation between protein thermostability and resistance to proteolysis.

R M Daniel, D A Cowan, H W Morgan, M P Curran
PMCID: PMC1153914  PMID: 6819862

Abstract

The loss of activity due to proteolysis of purified L-asparaginase and beta-galactosidase from different sources correlates with the thermal instability of the enzymes. A similar correlation is found when populations of soluble proteins from micro-organisms grown at different temperatures are compared for proteolytic susceptibility and thermal stability. It is proposed that there is a general correlation between the thermostability of proteins and their resistance to proteolysis.

Full text

PDF
644

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amelunxen R. E., Murdock A. L. Mechanisms of thermophily. CRC Crit Rev Microbiol. 1978;6(4):343–393. doi: 10.3109/10408417809090626. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. CRAVEN G. R., STEERS E., Jr, ANFINSEN C. B. PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12. J Biol Chem. 1965 Jun;240:2468–2477. [PubMed] [Google Scholar]
  4. Cowan D. A., Daniel R. M. Purification and some properties of an extracellular protease (caldolysin) from an extreme thermophile. Biochim Biophys Acta. 1982 Aug 10;705(3):293–305. doi: 10.1016/0167-4838(82)90251-5. [DOI] [PubMed] [Google Scholar]
  5. Daniel R. M., Appleby C. A. Anaerobic-nitrate, symbiotic and aerobic growth of Rhizobium japonicum: effects on cytochrome P 450 , other haemoproteins, nitrate and nitrite reductases. Biochim Biophys Acta. 1972 Sep 20;275(3):347–354. doi: 10.1016/0005-2728(72)90215-0. [DOI] [PubMed] [Google Scholar]
  6. Goldberg A. L., Dice J. F. Intracellular protein degradation in mammalian and bacterial cells. Annu Rev Biochem. 1974;43(0):835–869. doi: 10.1146/annurev.bi.43.070174.004155. [DOI] [PubMed] [Google Scholar]
  7. Grütter M. G., Hawkes R. B., Matthews B. W. Molecular basis of thermostability in the lysozyme from bacteriophage T4. Nature. 1979 Feb 22;277(5698):667–669. doi: 10.1038/277667a0. [DOI] [PubMed] [Google Scholar]
  8. Guy G. R., Daniel R. M. The purification and some properties of a stereospecific D-asparaginase from an extremely thermophilic bacterium, Thermus aquaticus. Biochem J. 1982 Jun 1;203(3):787–790. doi: 10.1042/bj2030787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KOFFLER H., GALE G. O. The relative thermostability of cytoplasmic proteins from thermophilic bacteria. Arch Biochem Biophys. 1957 Mar;67(1):249–251. doi: 10.1016/0003-9861(57)90267-9. [DOI] [PubMed] [Google Scholar]
  10. Langridge J. Genetic and enzymatic experiments relating to the tertiary structure of beta-galactosidase. J Bacteriol. 1968 Nov;96(5):1711–1717. doi: 10.1128/jb.96.5.1711-1717.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Matsunaga A., Noso Y. Conformational change with temperature and thermostability of glutamine synthetase from Bacillus stearothermophilus. Biochim Biophys Acta. 1974 Sep 13;365(1):208–211. doi: 10.1016/0005-2795(74)90265-7. [DOI] [PubMed] [Google Scholar]
  12. Matthews B. W., Weaver L. H., Kester W. R. The conformation of thermolysin. J Biol Chem. 1974 Dec 25;249(24):8030–8044. [PubMed] [Google Scholar]
  13. O'BRIEN R. T., CAMPBELL L. L., Jr Purification and properties of a proteolytic enzyme from Bacillus stearothermophilus. Arch Biochem Biophys. 1957 Aug;70(2):432–441. doi: 10.1016/0003-9861(57)90131-5. [DOI] [PubMed] [Google Scholar]
  14. Privalov P. L. Stability of proteins: small globular proteins. Adv Protein Chem. 1979;33:167–241. doi: 10.1016/s0065-3233(08)60460-x. [DOI] [PubMed] [Google Scholar]
  15. Ulrich J. T., McFeters G. A., Temple K. L. Induction and characterization of -galactosidase in an extreme thermophile. J Bacteriol. 1972 May;110(2):691–698. doi: 10.1128/jb.110.2.691-698.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES