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. 1983 Jan 15;210(1):21–28. doi: 10.1042/bj2100021

Subcellular localization and tissue distribution of sialic acid precursor-forming enzymes.

J Van Rinsum, W Van Dijk, G J Hooghwinkel, W Ferwerda
PMCID: PMC1154185  PMID: 6303311

Abstract

The enzymes UDP-N-acetylglucosamine pyrophosphorylase, UDP-N-acetylglucosamine 2-epimerase, N-acetylmannosamine kinase, N-acetylglucosamine kinase and N-acetylglucosamine 2-epimerase, which are involved in the metabolism of N-acetylneuraminic acid, were studied in rat with regard to their subcellular localization and tissue distribution. The subcellular distribution studies in liver indicated that the enzymes are localized in the soluble cell fraction. In other tissues the comparison of enzyme activities in homogenates with that in high-speed supernatants led to a similar conclusion. UDP-N-acetylglucosamine pyrophosphorylase, N-acetylglucosamine kinase and N-acetylglucosamine 2-epimerase were detected in almost all tissues studied. UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase, two enzymes considered to be key enzymes in the N-acetylneuraminic acid biosynthesis, were detected only in sialoglycoprotein-secreting tissues, i.e. liver, salivary gland and intestinal mucosa. The low activity of the key enzymes in other tissues suggests that the biosynthetic pathway of N-acetylneuraminic acid is not the same in various tissues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen M. B., Brockelbank J. L., Walker D. G. Apparent 'glucokinase' activity in non-hepatic tissues due to N-acetyl-D-glucosamine kinase. Biochim Biophys Acta. 1980 Aug 7;614(2):357–366. doi: 10.1016/0005-2744(80)90225-9. [DOI] [PubMed] [Google Scholar]
  2. Allen M. B., Walker D. G. Kinetic characterization of N-acetyl-D-glucosamine kinase from rat liver and kidney. Biochem J. 1980 Mar 1;185(3):577–582. doi: 10.1042/bj1850577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Allen M. B., Walker D. G. The isolation and preliminary characterization of N-acetyl-D-glucosamine kinase from rat kidney and liver. Biochem J. 1980 Mar 1;185(3):565–575. doi: 10.1042/bj1850565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BLACKLOW R. S., WARREN L. Biosynthesis of sialic acids by Neisseria meningitidis. J Biol Chem. 1962 Nov;237:3520–3526. [PubMed] [Google Scholar]
  5. BRUNETTI P., JOURDIAN G. W., ROSEMAN S. The sialic acids. III. Distribution and properties of animal N-acetylneuraminic aldolase. J Biol Chem. 1962 Aug;237:2447–2453. [PubMed] [Google Scholar]
  6. COMB D. G., ROSEMAN S. Enzymic synthesis of N-acetyl-D-mannosamine. Biochim Biophys Acta. 1958 Sep;29(3):653–654. doi: 10.1016/0006-3002(58)90031-3. [DOI] [PubMed] [Google Scholar]
  7. COMB D. G., ROSEMAN S. The sialic acids. I. The structure and enzymatic synthesis of N-acetylneuraminic acid. J Biol Chem. 1960 Sep;235:2529–2537. [PubMed] [Google Scholar]
  8. Carey D. J., Hirschberg C. B. Kinetics of glycosylation and intracellular transport of sialoglycoproteins in mouse liver. J Biol Chem. 1980 May 10;255(9):4348–4354. [PubMed] [Google Scholar]
  9. Carey D. J., Hirschberg C. B. Topography of sialoglycoproteins and sialyltransferases in mouse and rat liver Golgi. J Biol Chem. 1981 Jan 25;256(2):989–993. [PubMed] [Google Scholar]
  10. Coates S. W., Gurney T., Jr, Sommers L. W., Yeh M., Hirschberg C. B. Subcellular localization of sugar nucleotide synthetases. J Biol Chem. 1980 Oct 10;255(19):9225–9229. [PubMed] [Google Scholar]
  11. Creek K. E., Morré D. J. Translocation of cytidine 5'-monophosphosialic acid across Golgi apparatus membranes. Biochim Biophys Acta. 1981 May 6;643(2):292–305. doi: 10.1016/0005-2736(81)90075-4. [DOI] [PubMed] [Google Scholar]
  12. Datta A. Regulatory role of adenosine triphosphate on hog kidney N-acetyl-D-glucosamine 2-epimerase. Biochemistry. 1970 Aug 18;9(17):3363–3370. doi: 10.1021/bi00819a011. [DOI] [PubMed] [Google Scholar]
  13. Ferwerda W., Blok C. M., Heijlman J. Turnover of free sialic acid, CMP-sialic acid, and bound sialic acid in rat brain. J Neurochem. 1981 Apr;36(4):1492–1499. doi: 10.1111/j.1471-4159.1981.tb00591.x. [DOI] [PubMed] [Google Scholar]
  14. GHOSH S., ROSEMAN S. Enzymatic phosphorylation of N-acetyl-D-mannosamine. Proc Natl Acad Sci U S A. 1961 Jul 15;47:955–958. doi: 10.1073/pnas.47.7.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. GHOSH S., ROSEMAN S. THE SIALIC ACIDS. IV. N-ACYL--D-GLUCOSAMINE 6-PHOSPHATE 2-EPIMERASE. J Biol Chem. 1965 Apr;240:1525–1530. [PubMed] [Google Scholar]
  16. GHOSH S., ROSEMAN S. THE SIALIC ACIDS. V. N-ACYL-D-GLUCOSAMINE 2-EPIMERASE. J Biol Chem. 1965 Apr;240:1531–1536. [PubMed] [Google Scholar]
  17. Gielen W., Schaper R., Pink H. Die subzelluläre Anordnung und die Aktivität der Cytidinmonophosphat-N-acetylneuraminat-Synthetase im jungen Rattengehirn. Hoppe Seylers Z Physiol Chem. 1971 Oct;352(10):1291–1296. [PubMed] [Google Scholar]
  18. Gielen W., Schaper R., Pink H. Neuraminidase und Cytidinmonophosphat-N-acetylneuraminat-Synthetase in Rinderleukozyten. Hoppe Seylers Z Physiol Chem. 1970 Jun;351(6):768–770. [PubMed] [Google Scholar]
  19. Harms E., Kreisel W., Morris H. P., Reutter W. Biosynthesis of N-acetylneuraminic acid in Morris hepatomas. Eur J Biochem. 1973 Jan 15;32(2):254–262. doi: 10.1111/j.1432-1033.1973.tb02605.x. [DOI] [PubMed] [Google Scholar]
  20. JOURDIAN G. W., ROSEMAN S. The sialic acids. II. Preparation of N-glycolylhexosamines, N-glycolylhexosamine 6-phosphates, glycolyl coenzyme A, and glycolyl glutathione. J Biol Chem. 1962 Aug;237:2442–2446. [PubMed] [Google Scholar]
  21. KORNFELD S., KORNFELD R., NEUFELD E. F., O'BRIEN P. J. THE FEEDBACK CONTROL OF SUGAR NUCLEOTIDE BIOSYNTHESIS IN LIVER. Proc Natl Acad Sci U S A. 1964 Aug;52:371–379. doi: 10.1073/pnas.52.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kean E. L. Nuclear cytidine 5'-monophosphosialic acid synthetase. J Biol Chem. 1970 May 10;245(9):2301–2308. [PubMed] [Google Scholar]
  23. Kikuchi K., Kikuchi H., Tsuiki S. Activities of sialic acid-synthesizing enzymes in rat liver and rat and mouse tumors. Biochim Biophys Acta. 1971 Nov 12;252(2):357–368. doi: 10.1016/0304-4165(71)90017-1. [DOI] [PubMed] [Google Scholar]
  24. Kikuchi K., Tsuiki S. Metabolism of exogenous N-acetylglucosamine in extracts of rat kidney, liver and hepatoma. Biochim Biophys Acta. 1979 May 1;584(2):246–253. doi: 10.1016/0304-4165(79)90269-1. [DOI] [PubMed] [Google Scholar]
  25. Kikuchi K., Tsuiki S. Purification and properties of UDP-N-acetylglucosamine 2'-epimerase from rat liver. Biochim Biophys Acta. 1973 Nov 15;327(1):193–206. doi: 10.1016/0005-2744(73)90117-4. [DOI] [PubMed] [Google Scholar]
  26. Kundig W., Ghosh S., Roseman S. The sialic acids. VII. N-acyl-D-mannosamine kinase from rat liver. J Biol Chem. 1966 Dec 10;241(23):5619–5626. [PubMed] [Google Scholar]
  27. LELOIR L. F., CARDINI C. E., OLAVARRIA J. M. Phosphorylation of acetylhexosamines. Arch Biochem Biophys. 1958 Mar;74(1):84–91. doi: 10.1016/0003-9861(58)90201-7. [DOI] [PubMed] [Google Scholar]
  28. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  29. Lisman J. J., de Haan J., Overdijk B. Isolation of lysosomes from brain tissue. A separation method by means of alteration of mitochondrial and synaptosomal sedimentation properties. Biochem J. 1979 Jan 15;178(1):79–87. doi: 10.1042/bj1780079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Okamoto Y., Akamatsu N. Metabolism of sialic acid in regenerating rat liver. Biochem J. 1980 Jun 15;188(3):905–911. doi: 10.1042/bj1880905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. PATTABIRAMAN T. N., BACHHAWAT B. K. Purification of uridine diphosphoacetylglucosamine pyrophosphorylase from sheep brain. Biochim Biophys Acta. 1961 Jun 10;50:129–134. doi: 10.1016/0006-3002(61)91068-x. [DOI] [PubMed] [Google Scholar]
  32. PATTABIRAMAN T. N., VARMA T. N., BACHHAWAT B. K. ENZYMIC DEGRADATION OF URIDINE DIPHOSPHOACETYLGLUCOSAMINE. Biochim Biophys Acta. 1964 Mar 2;83:74–83. doi: 10.1016/0926-6526(64)90053-9. [DOI] [PubMed] [Google Scholar]
  33. PENNINGTON R. J. Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase. Biochem J. 1961 Sep;80:649–654. doi: 10.1042/bj0800649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. STROMINGER J. L., SMITH M. S. Uridine diphosphoacetylglucosamine pyrophosphorylase. J Biol Chem. 1959 Jul;234(7):1822–1827. [PubMed] [Google Scholar]
  35. Salo W. L., Fletcher H. G., Jr Studies onthe mechanism of action of uridine diphosphate N-acetylglucosamine 2-epimerase. Biochemistry. 1970 Feb 17;9(4):882–885. doi: 10.1021/bi00806a024. [DOI] [PubMed] [Google Scholar]
  36. Schachter H. The subcellular sites of glycosylation. Biochem Soc Symp. 1974;(40):57–71. [PubMed] [Google Scholar]
  37. Sommar K. M., Ellis D. B. Uridine diphosphate N-acetyl-D-glucosamine-2-epimerase from rat liver. I. Catalytic and regulatory properties. Biochim Biophys Acta. 1972 May 12;268(2):581–589. doi: 10.1016/0005-2744(72)90355-5. [DOI] [PubMed] [Google Scholar]
  38. WARREN L., FELSENFELD H. N-Acetylmannosamine-6-phosphate and N-acetylneuraminic acid-9-phosphate as intermediates in sialic acid biosynthesis. Biochem Biophys Res Commun. 1961 Jun 28;5:185–190. doi: 10.1016/0006-291x(61)90107-3. [DOI] [PubMed] [Google Scholar]
  39. WARREN L., FELSENFELD H. The biosynthesis of sialic acids. J Biol Chem. 1962 May;237:1421–1431. [PubMed] [Google Scholar]
  40. van Dijk W., Ferwerda W., van den Eijnden D. H. Subcellular and regional distribution of CMP-N-acetylneuraminic acid synthetase in the calf kidney. Biochim Biophys Acta. 1973 Jul 5;315(1):162–175. doi: 10.1016/0005-2744(73)90139-3. [DOI] [PubMed] [Google Scholar]
  41. van den Eijnden D. H., Meems L., Roukema P. A. The regional distribution of cytidine 5'-monophospho-N-acetyl-neuraminic acid synthetase in calf brain. J Neurochem. 1972 Jul;19(7):1649–1658. doi: 10.1111/j.1471-4159.1972.tb06210.x. [DOI] [PubMed] [Google Scholar]
  42. van den Eijnden D. H. The subcellular localization of cytidine 5'-monophospho-N-acetylneuraminic acid synthetase in calf brain. J Neurochem. 1973 Oct;21(4):949–958. doi: 10.1111/j.1471-4159.1973.tb07539.x. [DOI] [PubMed] [Google Scholar]

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