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. 1995 Sep 15;487(Pt 3):549–555. doi: 10.1113/jphysiol.1995.sp020899

Control of the amiloride-sensitive Na+ current in mouse salivary ducts by intracellular anions is mediated by a G protein.

A Dinudom 1, P Komwatana 1, J A Young 1, D I Cook 1
PMCID: PMC1156644  PMID: 8544120

Abstract

1. We have previously reported that the Na+ conductance in mouse intralobular salivary duct cells is controlled by cytosolic anions, being inhibited by high cytosolic concentrations of Cl- and NO3- but not of glutamate. In the present paper, we use whole-cell patch-clamp methods to investigate whether this anion effect is mediated by a G protein. 2. Inclusion of 100 mumol l-1 GTP-gamma-S, a non-hydrolysable GTP analogue, in the glutamate-containing pipette solution, i.e. when the Na+ conductance is active, reduced the size of the Na+ conductance whereas inclusion of 100 mumol l-1 GDP-beta-S, a non-hydrolysable GDP analogue, had no effect. 3. Inclusion of 100 mumol l-1 GDP-beta-S in the NO3(-)-containing pipette solution, i.e. when the Na+ conductance is inhibited, reactivated the conductance. Inclusion of 500 ng ml-1 activated pertussis toxin in the NO3(-)-containing pipette solution had a similar effect on the Na+ conductance. 4. We conclude that the inhibitory effect of intracellular anions such as NO3- and Cl- on the amiloride-sensitive Na+ conductance in mouse mandibular intralobular duct cells is mediated by a G protein sensitive to pertussis toxin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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