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. 1995 Nov 15;489(Pt 1):55–62. doi: 10.1113/jphysiol.1995.sp021029

Ca2+ current enhancement by alpha 2/delta and beta subunits in Xenopus oocytes: contribution of changes in channel gating and alpha 1 protein level.

E Shistik 1, T Ivanina 1, T Puri 1, M Hosey 1, N Dascal 1
PMCID: PMC1156791  PMID: 8583415

Abstract

1. A combined biochemical and electrophysiological approach was used to determine the mechanism by which the auxiliary subunits of Ca2+ channel enhance the macroscopic Ca2+ currents. Xenopus oocytes were injected with RNA of the main pore-forming subunit (cardiac: alpha 1C), and various combinations of RNAs of the auxiliary subunits (alpha 2/delta and beta 2A). 2. The single channel open probability (Po; measured at 0 mV) was increased approximately 3-, approximately 8- and approximately 35-fold by alpha 2/delta, beta 2A and alpha 2/delta+beta 2A, respectively. The whole-cell Ca2+ channel current was increased approximately 8- to 10-fold by either alpha 2/delta or beta 2A, and synergistically > 100-fold by alpha 2/delta+beta 2A. The amount of 35S-labelled alpha 1 protein in the plasma membrane was not changed by coexpression of beta 2A, but was tripled by coexpression of alpha 2/delta (either with or without beta). 3. We conclude that the increase in macroscopic current by alpha 2/delta is equally due to changes in amount of alpha 1 in the plasma membrane and an increase in Po, whereas all of the effect of beta 2A is due to an increase in Po. The synergy between alpha 2/delta and beta in increasing the macroscopic current is due mainly to synergistic changes in channel gating.

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Selected References

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