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. 1982 Jan 15;202(1):47–51. doi: 10.1042/bj2020047

Decreased protein and puromycinyl-peptide degradation in livers of senescent mice.

L Lavie, A Z Reznick, D Gershon
PMCID: PMC1158072  PMID: 7082317

Abstract

Liver protein-degradation rates were determined in young and old C57B1 mice by the method of Swick & Ip [(1974) J. Biol. Chem. 249, 6836-6841]. The results indicated a marked age-related increase in the half-lives of short-lived proteins in the nuclear, mitochondrial, lysosomal and 100000 g-supernatant cellular fractions and in total trichloroacetic acid-precipitable proteins. The efficiency of the degradation system in removing aberrant proteins from livers of young and old mice was tested. The time required for 50% disappearance of puromycinyl-peptides changed from about 20 min in 6-month-old mice to approx. 150 min in 24-month-old animals. These findings suggest that in old animals the proteolytic activity involved in degradation of aberrant proteins, and presumably of "native proteins, is markedly defective.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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