Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1982 Jan 15;202(1):139–143. doi: 10.1042/bj2020139

Transacylation as a chain-termination mechanism in fatty acid synthesis by mammalian fatty acid synthetase. Synthesis of medium-chain-length (C8-C12) acyl-CoA esters by goat mammary-gland fatty acid synthetase.

J Knudsen, I Grunnet
PMCID: PMC1158083  PMID: 7082303

Abstract

1. Ruminant mammary-gland fatty acid synthetases can, in contrast with non-ruminant mammary enzymes, synthesize medium-chain fatty acids. 2. Medium-chain fatty acids are only synthesized in the presence of a fatty acid-removing system such as albumin, beta-lactoglobulin or methylated cyclodextrin. 3. The short- and medium-chain fatty acids synthesized were released as acyl-CoA esters from the fatty acid synthetase.

Full text

PDF
141

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EGGERER H., LYNEN F. [On the biosynthesis of fatty acids. II. Synthesis and properties of S-malonyl-coenzyme A]. Biochem Z. 1962;335:540–547. [PubMed] [Google Scholar]
  2. Engeser H., Hübner K., Straub J., Lynen F. Identity of malonyl and palmitoyl transferase of fatty acid synthetase from yeast. 2. A comparison of active-site peptides. Eur J Biochem. 1979 Nov;101(2):413–422. doi: 10.1111/j.1432-1033.1979.tb19734.x. [DOI] [PubMed] [Google Scholar]
  3. Grunnet I., Knudsen J. Direct transfer of fatty acids synthesized "de novo" from fatty acid synthetase into triacylglycerols without activation. Biochem Biophys Res Commun. 1981 May 29;100(2):629–636. doi: 10.1016/s0006-291x(81)80222-7. [DOI] [PubMed] [Google Scholar]
  4. Grunnet I., Knudsen J. Fatty-acid synthesis in lactating-goat mammary gland. 1. Medium-chain fatty-acid synthesis. Eur J Biochem. 1979 Apr;95(3):497–502. doi: 10.1111/j.1432-1033.1979.tb12989.x. [DOI] [PubMed] [Google Scholar]
  5. Grunnet I., Knudsen J. Fatty-acid synthesis in lactating-goat mammary gland. 2. Medium-chain fatty acid synthesis. Eur J Biochem. 1979 Apr;95(3):503–507. doi: 10.1111/j.1432-1033.1979.tb12990.x. [DOI] [PubMed] [Google Scholar]
  6. Grunnet I., Knudsen J. Medium chain acyl-thioester hydrolase activity in goat and rabbit mammary gland fatty acid synthetase complexes. Biochem Biophys Res Commun. 1978 Feb 28;80(4):745–749. doi: 10.1016/0006-291x(78)91308-6. [DOI] [PubMed] [Google Scholar]
  7. Knudsen J., Clark S., Dils R. Purification and some properties of a medium-chain acyl-thioester hydrolase from lactating-rabbit mammary gland which terminates chain elongation in fatty acid synthesis. Biochem J. 1976 Dec 15;160(3):683–691. doi: 10.1042/bj1600683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Knudsen J. Fatty acid synthetase from cow mammary gland tissue cells. Biochim Biophys Acta. 1972 Nov 30;280(3):408–414. doi: 10.1016/0005-2760(72)90246-9. [DOI] [PubMed] [Google Scholar]
  9. Knudsen J., Grunnet I. Primer specificity of mammalian mammary gland fatty acid synthetases. Biochem Biophys Res Commun. 1980 Aug 29;95(4):1808–1814. doi: 10.1016/s0006-291x(80)80109-4. [DOI] [PubMed] [Google Scholar]
  10. Knudsen J., Hansen J. K., Grunnet I. Quantitative extraction and separation from [1-14C]acetate and preparation for ratio gas chromatography of microgram amounts of a 14C-labeled mixture of butyrate and longer-chain-length fatty acids. Anal Biochem. 1981 Mar 15;112(1):190–193. doi: 10.1016/0003-2697(81)90279-7. [DOI] [PubMed] [Google Scholar]
  11. Libertini L. J., Smith S. Purification and properties of a thioesterase from lactating rat mammary gland which modifies the product specificity of fatty acid synthetase. J Biol Chem. 1978 Mar 10;253(5):1393–1401. [PubMed] [Google Scholar]
  12. Linn T. C., Srere P. A. Coenzyme A requirement for the termination reaction of rat liver fatty acid synthetase. J Biol Chem. 1980 Nov 25;255(22):10676–10680. [PubMed] [Google Scholar]
  13. Machida Y., Bergeron R., Flick P., Bloch K. Effects of cyclodextrins on fatty acid synthesis. J Biol Chem. 1973 Sep 10;248(17):6246–6247. [PubMed] [Google Scholar]
  14. Spector A. A., Fletcher J. E. Binding of long chain fatty acids to beta-lactoglobulin. Lipids. 1970 Apr;5(4):403–411. doi: 10.1007/BF02532106. [DOI] [PubMed] [Google Scholar]
  15. Spector A. A., John K., Fletcher J. E. Binding of long-chain fatty acids to bovine serum albumin. J Lipid Res. 1969 Jan;10(1):56–67. [PubMed] [Google Scholar]
  16. Sumper M., Oesterhelt D., Riepertinger C., Lynen F. Die Synthese verschiedener Carbonsäuren durch den Multienzymkomplex der Fettsäuresynthese aus Hefe und die Erklärung ihrer Bildung. Eur J Biochem. 1969 Sep;10(2):377–387. doi: 10.1111/j.1432-1033.1969.tb00701.x. [DOI] [PubMed] [Google Scholar]
  17. Wood W. I., Peterson D. O., Bloch K. Mycobacterium smegmatis fatty acid synthetase. A mechanism based on steady state rates and product distributions. J Biol Chem. 1977 Aug 25;252(16):5745–5749. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES