Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1982 Jan 15;202(1):243–247. doi: 10.1042/bj2020243

Lipolysis in rat adipocytes during pregnancy and lactation. The response to noradrenaline.

R E Aitchison, R A Clegg, R G Vernon
PMCID: PMC1158097  PMID: 6282271

Abstract

1. Lipolysis has been measured in parametrial adipocytes from virgin, pregnant and lactating rats. 2. The basal rate and the maximal rate of lipolysis, the latter measured in the presence of noradrenaline and theophylline, remained constant between the three experimental categories, with the exception of a significant transient increase in the basal rate at parturition. 3. The noradrenaline-stimulated lipolysis rate rose above the virgin rate during pregnancy and fell below it during lactation; inclusion of adenosine deaminase in incubations abolished these differences in response to noradrenaline. 4. Cyclic AMP phosphodiesterase activity was lower in adipocytes during pregnancy and lactation than in virgin animals.

Full text

PDF
246

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEATON G. H., BEARE J., RYU M. H., McHENRY E. W. Protein metabolism in the pregnant rat. J Nutr. 1954 Oct 11;54(2):291–304. doi: 10.1093/jn/54.2.291. [DOI] [PubMed] [Google Scholar]
  2. Burton L. E., Wells W. W. Characterization of the lactation-dependent fatty liver in myo-inositol deficient rats. J Nutr. 1977 Oct;107(10):1871–1883. doi: 10.1093/jn/107.10.1871. [DOI] [PubMed] [Google Scholar]
  3. Farid M., Baldwin R. L., Yang Y. T., Osborne E., Grichting G. Effects of age, diet and lactation on lipogenesis in rat adipose, liver and mammary tissues. J Nutr. 1978 Mar;108(3):514–524. doi: 10.1093/jn/108.3.514. [DOI] [PubMed] [Google Scholar]
  4. Felber J. P., Zaragoza N., Benuzzi-Badoni M., Genazzani A. R. The double effect of human chorionic somatomammotropin (HCS) and pregnancy on lipogenesis and on lipolysis in the isolated rat epididymal fat pad and fat pad cells. Horm Metab Res. 1972 Jul;4(4):293–296. doi: 10.1055/s-0028-1094070. [DOI] [PubMed] [Google Scholar]
  5. Fernandez B. M., Saggerson E. D. Alterations in response of rat white adipocytes to insulin, noradrenaline, corticotropin and glucagon after adrenalectomy. Correction of these changes by adenosine deaminase. Biochem J. 1978 Jul 15;174(1):111–118. doi: 10.1042/bj1740111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flint D. J., Sinnett-Smith P. A., Clegg R. A., Vernon R. G. Role of insulin receptors in the changing metabolism of adipose tissue during pregnancy and lactation in the rat. Biochem J. 1979 Aug 15;182(2):421–427. doi: 10.1042/bj1820421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fredholm B. B. Local regulation of lipolysis in adipose tissue by fatty acids, prostaglandins and adenosine. Med Biol. 1978 Oct;56(5):249–261. [PubMed] [Google Scholar]
  8. Fredholm B. B., Sollevi A. The release of adenosine and inosine from canine subcutaneous adipose tissue by nerve stimulation and noradrenaline. J Physiol. 1981;313:351–367. doi: 10.1113/jphysiol.1981.sp013670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GARLAND P. B., RANDLE P. J. A rapid enzymatic assay for glycerol. Nature. 1962 Dec 8;196:987–988. doi: 10.1038/196987a0. [DOI] [PubMed] [Google Scholar]
  10. Hales C. N., Luzio J. P., Siddle K. Hormonal control of adipose-tissue lipolysis. Biochem Soc Symp. 1978;(43):97–135. [PubMed] [Google Scholar]
  11. Hamosh M., Clary T. R., Chernick S. S., Scow R. O. Lipoprotein lipase activity of adipose and mammary tissue and plasma triglyceride in pregnant and lactating rats. Biochim Biophys Acta. 1970 Sep 8;210(3):473–482. doi: 10.1016/0005-2760(70)90044-5. [DOI] [PubMed] [Google Scholar]
  12. Hanson R. W., Ballard F. J. Citrate, pyruvate, and lactate contaminants of commercial serum albumin. J Lipid Res. 1968 Sep;9(5):667–668. [PubMed] [Google Scholar]
  13. Honnor R. C., Saggerson E. D. Altered lipolytic response to glucagon and adenosine deaminase in adipocytes from starved rats. Biochem J. 1980 Jun 15;188(3):757–761. doi: 10.1042/bj1880757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Knopp R. H., Herrera E., Freinkel N. Carbohydrate metabolism in pregnancy. 8. Metabolism of adipose tissue isolated from fed and fasted pregnant rats during late gestation. J Clin Invest. 1970 Jul;49(7):1438–1446. doi: 10.1172/JCI106361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Knopp R. H., Saudek C. D., Arky R. A., O'Sullivan J. B. 2 phases of adipose tissue metabolism in pregnancy: maternal adaptations for fetal growth. Endocrinology. 1973 Apr;92(4):984–988. doi: 10.1210/endo-92-4-984. [DOI] [PubMed] [Google Scholar]
  16. Lorenzo M., Caldés T., Benito M., Medina J. M. Lipogenesis in vivo in maternal and foetal tissues during late gestation in the rat. Biochem J. 1981 Aug 15;198(2):425–428. doi: 10.1042/bj1980425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ohisalo J. J., Stouffer J. E. Adenosine, thyroid status and regulation of lipolysis. Biochem J. 1979 Jan 15;178(1):249–251. doi: 10.1042/bj1780249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Otway S., Robinson D. S. The significance of changes in tissue clearing-factor lipase activity in relation to the lipaemia of pregnancy. Biochem J. 1968 Feb;106(3):677–682. doi: 10.1042/bj1060677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Robinson A. M., Girard J. R., Williamson D. H. Evidence for a role of insulin in the regulation of lipogenesis in lactating rat mammary gland. Measurements of lipogenesis in vivo and plasma hormone concentrations in response to starvation and refeeding. Biochem J. 1978 Oct 15;176(1):343–346. doi: 10.1042/bj1760343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SPRAY C. M. A study of some aspects of reproduction by means of chemical analysis. Br J Nutr. 1950;4(4):354–360. doi: 10.1079/bjn19500059. [DOI] [PubMed] [Google Scholar]
  21. Schwabe U., Ebert R., Erbler H. C. Adenosine release from fat cells: effect on cyclic AMP levels and hormone actions. Adv Cyclic Nucleotide Res. 1975;5:569–584. [PubMed] [Google Scholar]
  22. Schwabe U., Ebert R., Erbler H. C. Adenosine release from isolated fat cells and its significance for the effects of hormones on cyclic 3',5'-AMP levels and lipolysis. Naunyn Schmiedebergs Arch Pharmacol. 1973;276(2):133–148. doi: 10.1007/BF00501186. [DOI] [PubMed] [Google Scholar]
  23. Smith R. W. The effects of pregnancy, lactation and involution on the metabolism of glucose by rat parametrial adipose tissue. J Dairy Res. 1973 Oct;40(3):353–360. doi: 10.1017/s0022029900014722. [DOI] [PubMed] [Google Scholar]
  24. Smith R. W., Walsh A. Effect of lactation on lipolysis in rat adipose tissue. Lipids. 1976 May;11(5):418–420. doi: 10.1007/BF02532850. [DOI] [PubMed] [Google Scholar]
  25. Sollevi A., Fredholm B. B. Role of adenosine in adipose tissue circulation. Acta Physiol Scand. 1981 Jul;112(3):293–298. doi: 10.1111/j.1748-1716.1981.tb06819.x. [DOI] [PubMed] [Google Scholar]
  26. Thompson W. J., Terasaki W. L., Epstein P. M., Strada S. J. Assay of cyclic nucleotide phosphodiesterase and resolution of multiple molecular forms of the enzyme. Adv Cyclic Nucleotide Res. 1979;10:69–92. [PubMed] [Google Scholar]
  27. Vernon R. G. Development of perirenal adipose tissue in the neonatal lamb: effects of dietary safflower oil. Biol Neonate. 1977;32(1-2):15–23. doi: 10.1159/000240991. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES