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. 1982 Mar 15;202(3):581–587. doi: 10.1042/bj2020581

Studies of lactate dehydrogenase in the purified state and in intact erythrocytes.

R J Simpson, K M Brindle, F F Brown, I D Campbell, D L Foxall
PMCID: PMC1158151  PMID: 7092832

Abstract

Lactate dehydrogenase in intact erythrocytes was studied by observing isotope exchange between lactate and pyruvate by p.m.r. The inhibition of the enzyme in intact cells by both oxalate and pyruvate was found to be similar to that of the purified enzyme. The activity of the enzyme in intact cells indicates that the free solution NAD+ + NADH concentration in erythrocytes is about 10 microM whereas the total extractable NAD+ + NADH is about 80 nmol/ml of cell water.

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Selected References

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