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. 1982 Sep 1;205(3):567–573. doi: 10.1042/bj2050567

Identification and purification of a liver microsomal glucose 6-phosphatase.

A Burchell, B Burchell
PMCID: PMC1158522  PMID: 6293458

Abstract

1. Hepatic glucose 6-phosphatase activity was purified 65-fold in good yield over that in cholate-solubilized microsomal fractions. 2. This preparation still contained five major polypeptides and numerous minor contaminants. 3. The smallest of the five major polypeptides (Mr approx. 18 500) could be purified from heat-treated microsomal fractions. 4. Antisera raised against the heat-stable protein doublet was used to immunoprecipitate specifically glucose 6-phosphatase activity from cholate-solubilized microsomal fractions. 5. This work indicates that hepatic microsomal glucose 6-phosphatase appears to be one or both of the low-molecular-weight heat-stable polypeptides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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