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. 1982 Aug 15;206(2):427–430. doi: 10.1042/bj2060427

Regulation of 3-hydroxybutyrate formation and secretion of very-low-density-lipoprotein triacylglycerol by perfused livers from fed and starved rats.

M E Laker, P A Mayes
PMCID: PMC1158602  PMID: 7150254

Abstract

Acetoacetate was the sole ketone body formed when livers from starved rats were perfused with minimal concentrations of non-esterified fatty acid. Absence of 3-hydroxybutyrate was related to a low substrate potential, which caused a more oxidized redox state and a decreased [ATP]/[ADP] ratio. Only under conditions of comparable non-esterified fatty acid uptake was lipoprotein triacylglycerol production inversely related to ketogenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brunengraber H., Boutry M., Lowenstein J. M. Fatty acid and 3- -hydroxysterol synthesis in the perfused rat liver. Including measurements on the production of lactate, pyruvate, -hydroxy-butyrate, and acetoacetate by the fed liver. J Biol Chem. 1973 Apr 25;248(8):2656–2669. [PubMed] [Google Scholar]
  2. Flatt J. P. On the maximal possible rate of ketogenesis. Diabetes. 1972 Jan;21(1):50–53. doi: 10.2337/diab.21.1.50. [DOI] [PubMed] [Google Scholar]
  3. Ide T., Ontko J. A. Increased secretion of very low density lipoprotein triglyceride following inhibition of long chain fatty acid oxidation in isolated rat liver. J Biol Chem. 1981 Oct 25;256(20):10247–10255. [PubMed] [Google Scholar]
  4. Laker M. E., Mayes P. A. Effect of hyperthyroidism and hypothyroidism on lipid and carbohydrate metabolism of the perfused rat liver. Biochem J. 1981 Apr 15;196(1):247–255. doi: 10.1042/bj1960247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Laker M. E., Mayes P. A. The immediate and long term effects of clofibrate on the metabolism of the perfused rat liver. Biochem Pharmacol. 1979 Sep 15;28(18):2813–2827. doi: 10.1016/0006-2952(79)90567-7. [DOI] [PubMed] [Google Scholar]
  6. Mayes P. A., Felts J. M. Regulation of fat metabolism of the liver. Nature. 1967 Aug 12;215(5102):716–718. doi: 10.1038/215716a0. [DOI] [PubMed] [Google Scholar]
  7. Mayes P. A., Laker M. E. Regulation of ketogenesis in the liver. Biochem Soc Trans. 1981 Aug;9(4):339–341. doi: 10.1042/bst0090339. [DOI] [PubMed] [Google Scholar]
  8. Ontko J. A. Metabolism of free fatty acids in isolated liver cells. Factors affecting the partition between esterification and oxidation. J Biol Chem. 1972 Mar 25;247(6):1788–1800. [PubMed] [Google Scholar]
  9. Shepherd D., Yates D. W., Garland P. B. The relationship between the rates of conversion of palmitate into citrate or acetoacetate and the acetyl-coenzyme A content of rat-liver mitochondria. Biochem J. 1965 Dec;97(3):38C–40C. doi: 10.1042/bj0970038c. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Siess E. A., Kientsch-Engel R. I., Wieland O. H. Role of free oxaloacetate in ketogenesis. Derivation from the direct measurement of mitochondrial [3-hydroxybutyrate]/[acetoacetate] ratio in hepatocytes. Eur J Biochem. 1982 Jan;121(3):493–499. doi: 10.1111/j.1432-1033.1982.tb05814.x. [DOI] [PubMed] [Google Scholar]
  11. Williamson J. R., Scholz R., Browning E. T. Control mechanisms of gluconeogenesis and ketogenesis. II. Interactions between fatty acid oxidation and the citric acid cycle in perfused rat liver. J Biol Chem. 1969 Sep 10;244(17):4617–4627. [PubMed] [Google Scholar]

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