Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1982 Sep 15;206(3):545–553. doi: 10.1042/bj2060545

Changes in the blood level and affinity to concanavalin A of rat plasma glycoproteins during acute inflammation and hepatoma growth.

A Koj, A Dubin, H Kasperczyk, J Bereta, A H Gordon
PMCID: PMC1158622  PMID: 7150262

Abstract

Plasma concentrations of ten individual proteins were measured by electroimmunoassay in young male Buffalo rats following injection of turpentine oil or implantation of Morris hepatoma 7777. The highest relative responses to inflammation and tumour growth were found for alpha 2-macroglobulin, alpha 1-acute-phase globulin and alpha 1-acid glycoprotein. As shown by crossed immuno-affinoelectrophoresis the concanavalin A-reactive fractions of the latter two glycoproteins were predominantly increased in plasma from injured and tumour-bearing rats.

Full text

PDF
549

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abd-el-Fattah M., Scherer R., Fouad F. M., Ruhenstroth-Bauer G. Kinetics of the acute-phase reaction in rats after tumor transplantation. Cancer Res. 1981 Jun;41(6):2548–2555. [PubMed] [Google Scholar]
  2. Berger E. G., Wyss S. R., Nimberg R. B., Schmid K. The microheterogeneity of human plasma alpha1-acid glycoprotein. Hoppe Seylers Z Physiol Chem. 1980 Oct;361(10):1567–1572. doi: 10.1515/bchm2.1980.361.2.1567. [DOI] [PubMed] [Google Scholar]
  3. Bosanquet A. G., Chandler A. M., Gordon A. H. Effects of injury on the concentration of alpha1-macroglobulin and alpha2-macroglobulin in the plasmas of male and remale rats. Experientia. 1976 Oct 15;32(10):1348–1349. doi: 10.1007/BF01953133. [DOI] [PubMed] [Google Scholar]
  4. Clarke H. G., Freeman T. Quantitative immunoelectrophoresis of human serum proteins. Clin Sci. 1968 Oct;35(2):403–413. [PubMed] [Google Scholar]
  5. DARCY D. A. RESPONSE OF A SERUM GLYCOPROTEIN TO TISSUE INJURY AND NECROSIS. I. THE RESPONSE TO NECROSIS, HYPERPLASIA AND TUMOUR GROWTH. Br J Exp Pathol. 1964 Jun;45:281–293. [PMC free article] [PubMed] [Google Scholar]
  6. Dobryszycka W., Krawczyk E. Microheterogeneity of mammalian haptoglobins in isoelectric focusing. Comp Biochem Physiol B. 1979;62(1):111–113. doi: 10.1016/0305-0491(79)90022-1. [DOI] [PubMed] [Google Scholar]
  7. Fournet B., Montreuil J., Strecker G., Dorland L., Haverkamp J., Vliegenthart F. G., Binette J. P., Schmid K. Determination of the primary structures of 16 asialo-carbohydrate units derived from human plasma alpha 1-acid glycoprotein by 360-MHZ 1H NMR spectroscopy and permethylation analysis. Biochemistry. 1978 Nov 28;17(24):5206–5214. doi: 10.1021/bi00617a021. [DOI] [PubMed] [Google Scholar]
  8. Friesen A. D., Jamieson J. C. Studies on incorporation of mannose into rat alpha 1-acid glycoprotein: evidence for precursor forms in rough membranes. Can J Biochem. 1980 Oct;58(10):1101–1111. doi: 10.1139/o80-148. [DOI] [PubMed] [Google Scholar]
  9. Gordon A. H., Koj A. Changes in the rates of synthesis of certain plasma proteins following tissue damage due to talc injection. A study using the perfused rat liver. Br J Exp Pathol. 1968 Oct;49(5):436–447. [PMC free article] [PubMed] [Google Scholar]
  10. Gordon A. H., Louis L. N. Alpha-1 acute-phase globulins of rats. Isolation and some properties. Biochem J. 1969 Jul;113(3):481–488. doi: 10.1042/bj1130481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jamieson J. C., Morrison K. E., Molasky D., Turchen B. Studies on acute phase proteins of rat serum. V Effect on induces inflammation on the synthesis of albumin and alpha-1-acid glycoprotein by liver slices. Can J Biochem. 1975 Apr;53(4):401–414. doi: 10.1139/o75-056. [DOI] [PubMed] [Google Scholar]
  12. Koj A. Comparison of synthesis and secretion of plasma albumin, fibrinogen and alpha 2-macroglobulin by slices of Morris hepatomas and rat liver. Br J Exp Pathol. 1980 Jun;61(3):332–338. [PMC free article] [PubMed] [Google Scholar]
  13. Koj A., Dubin A. The effect of D-galactosamine on plasma protein synthesis by the perfused rat liver from turpentine-stimulated donors. Br J Exp Pathol. 1978 Oct;59(5):504–513. [PMC free article] [PubMed] [Google Scholar]
  14. Koj A., Regoeczi E. Effect of experimental inflammation on the synthesis and distribution of antithrombin III and alpha1-antitrypsin in rabbits. Br J Exp Pathol. 1978 Oct;59(5):473–481. [PMC free article] [PubMed] [Google Scholar]
  15. LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
  16. Nagashima M., Urban J., Schreiber G. Intrahepatic precursor form of rat alpha 1-acid glycoprotein. Isolation and properties. J Biol Chem. 1980 May 25;255(10):4951–4956. [PubMed] [Google Scholar]
  17. Nicollet I., Lebreton J. P., Fontaine M., Hiron M. Evidence for alpha-1-acid glycoprotein populations of different pI values after concanavalin A affinity chromatography. Study of their evolution during inflammation in man. Biochim Biophys Acta. 1981 Apr 28;668(2):235–245. doi: 10.1016/0005-2795(81)90031-3. [DOI] [PubMed] [Google Scholar]
  18. Okubo H., Miyanaga O., Nagano M., Ishibashi H., Kudo J., Ikuta T., Shibata K. Purification and immunological determination of alpha 2-macroglobulin in serum from injured rats. Biochim Biophys Acta. 1981 Apr 28;668(2):257–267. doi: 10.1016/0005-2795(81)90033-7. [DOI] [PubMed] [Google Scholar]
  19. Schmid K., Binette J. P., Dorland L., Vliegenthart J. F., Fournet B., Montreuil J. The primary structure of the asialo-carbohydrate units of the first glycosylation site of human plasma alpha 1-acid glycoprotein. Biochim Biophys Acta. 1979 Dec 14;581(2):356–359. doi: 10.1016/0005-2795(79)90255-1. [DOI] [PubMed] [Google Scholar]
  20. Schreiber G., Boutwell R. K., Potter V. R., Morris H. P. Lack of secretion of serum protein by transplanted rat hepatomas. Cancer Res. 1966 Nov;26(11):2357–2361. [PubMed] [Google Scholar]
  21. Shibata K., Okubo H., Ishibashi H., Tsuda K. Rat alpha1-acid glycoprotein. Purification and immunological estimation of its serum concentration. Biochim Biophys Acta. 1977 Nov 25;495(1):37–45. doi: 10.1016/0005-2795(77)90237-9. [DOI] [PubMed] [Google Scholar]
  22. Snyder S., Ashwell G. Quantitation of specific serum glycoproteins in malignancy. Clin Chim Acta. 1971 Oct;34(3):449–455. doi: 10.1016/0009-8981(71)90100-8. [DOI] [PubMed] [Google Scholar]
  23. Urban J., Chan D., Schreiber G. A rat serum glycoprotein whose synthesis rate increases greatly during inflammation. J Biol Chem. 1979 Nov 10;254(21):10565–10568. [PubMed] [Google Scholar]
  24. Weimer H. E., Coggshall V. Divergent responses of serum glycoprotein fractions to tissue injury in adrenalectomized rats. Can J Physiol Pharmacol. 1967 Sep;45(5):767–775. doi: 10.1139/y67-091. [DOI] [PubMed] [Google Scholar]
  25. Weimer H. E., Humelbaugh C., Roberts D. M. The influence of tumor growth on the synthesis of alpha 2-AP (acute phase) globulin of rat serum. Can J Biochem. 1967 Dec;45(12):1937–1941. doi: 10.1139/o67-226. [DOI] [PubMed] [Google Scholar]
  26. Wells C., Bøg-Hansen T. C., Cooper E. H., Glass M. R. The use of concanavalin A crossed immuno-affinoelectrophoresis to detect hormone-associated variations in alpha 1-acid glycoprotein. Clin Chim Acta. 1981 Jan 8;109(1):59–67. doi: 10.1016/0009-8981(81)90137-6. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES