Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1982 Sep 15;206(3):647–654. doi: 10.1042/bj2060647

Hydrolysis of plasma triacylglycerol-rich lipoproteins from immature and laying hens (Gallus domesticus) by lipoprotein lipase in vitro.

H Griffin, G Grant, M Perry
PMCID: PMC1158635  PMID: 7150269

Abstract

Very-low-density (VLD) lipoproteins and portomicrons were isolated from the plasma of immature and laying hens and their size, lipid composition and susceptibility to hydrolysis by lipoprotein lipase were compared. In agreement with other studies, VLD lipoproteins from laying hens were found to be smaller and have a different lipid composition than VLD lipoproteins from immature hens. Portomicrons from immature and laying hens had mean diameters of about 150 nm and similar lipid compositions. Hydrolysis of VLD lipoproteins from immature hens, and portomicrons from immature and laying hens, proceeded rapidly until at least 40% of the substrate had been used. In contrast only 1--15% of laying-hen VLD-lipoprotein triacylglycerol was readily hydrolysis occurred slowly. The limited susceptibility of laying-hen VLD lipoproteins appeared to be due to their low content of lipoprotein lipase activator apoprotein, which occurred despite an abundance of activator in the high-density lipoproteins of laying-hen plasma. The results provide further evidence that the liver of the laying hen synthesizes specialized lipoproteins. Their limited susceptibility to hydrolysis by lipoprotein lipase is probably a major factor in ensuring transport of lipid to yolk rather than to other tissues. The form of transport of dietary lipid, however, is similar in immature and laying hens.

Full text

PDF
654

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allain C. C., Poon L. S., Chan C. S., Richmond W., Fu P. C. Enzymatic determination of total serum cholesterol. Clin Chem. 1974 Apr;20(4):470–475. [PubMed] [Google Scholar]
  2. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  3. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  4. Bensadoun A., Ehnholm C., Steinberg D., Brown W. V. Purification and characterization of lipoprotein lipase from pig adipose tissue. J Biol Chem. 1974 Apr 10;249(7):2220–2227. [PubMed] [Google Scholar]
  5. Bensadoun A., Kompiang I. P. Role of lipoprotein lipase in plasma triglyceride removal. Fed Proc. 1979 Nov;38(12):2622–2626. [PubMed] [Google Scholar]
  6. Bensadoun A., Rothfeld A. The form of absorption of lipids in the chicken, Gallus domesticus. Proc Soc Exp Biol Med. 1972 Dec;141(3):814–817. doi: 10.3181/00379727-141-36878. [DOI] [PubMed] [Google Scholar]
  7. Breckenridge W. C., Little J. A., Steiner G., Chow A., Poapst M. Hypertriglyceridemia associated with deficiency of apolipoprotein C-II. N Engl J Med. 1978 Jun 8;298(23):1265–1273. doi: 10.1056/NEJM197806082982301. [DOI] [PubMed] [Google Scholar]
  8. Caffrey M., Feigenson G. W. Fatty-acyl-chain characteristics of phosphatidylcholines affect Ca2+-dependent ATPase enzymic activity but not the affinity of the protein for these different lipid species. Biochem Soc Trans. 1981 Feb;9(1):155–156. doi: 10.1042/bst0090155. [DOI] [PubMed] [Google Scholar]
  9. Chan L., Jackson R. L., O'Malley B. W., Means A. R. Synthesis of very low density lipoproteins in the cockerel. Effects of estrogen. J Clin Invest. 1976 Aug;58(2):368–379. doi: 10.1172/JCI108481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chapman M. J. Animal lipoproteins: chemistry, structure, and comparative aspects. J Lipid Res. 1980 Sep;21(7):789–853. [PubMed] [Google Scholar]
  11. Chapman M. J., Goldstein S., Laudat M. H. Characterization and comparative aspects of the serum very low and low density lipoproteins and their apoproteins in the chicken (Gallus domesticus). Biochemistry. 1977 Jun 28;16(13):3006–3015. doi: 10.1021/bi00632a031. [DOI] [PubMed] [Google Scholar]
  12. Chen R. F. Removal of fatty acids from serum albumin by charcoal treatment. J Biol Chem. 1967 Jan 25;242(2):173–181. [PubMed] [Google Scholar]
  13. Chung J., Scanu A. M. Continuous pH-stat titration method for the assay of lipoprotein lipase activity in vitro. Anal Biochem. 1974 Nov;62(1):134–148. doi: 10.1016/0003-2697(74)90374-1. [DOI] [PubMed] [Google Scholar]
  14. DOLE V. P., MEINERTZ H. Microdetermination of long-chain fatty acids in plasma and tissues. J Biol Chem. 1960 Sep;235:2595–2599. [PubMed] [Google Scholar]
  15. Fitzharris T. J., Quinn D. M., Goh E. H., Johnson J. D., Kashyap M. L., Srivastava L. S., Jackson R. L., Harmony J. A. Hydrolysis of guinea pig nascent very low density lipoproteins catalyzed by lipoprotein lipase: activation by hjman apolipoprotein C-II. J Lipid Res. 1981 Aug;22(6):921–933. [PubMed] [Google Scholar]
  16. Gornall D. A., Kuksis A. Alterations in lipid composition of plasma lipoproteins during deposition of egg yolk. J Lipid Res. 1973 Mar;14(2):197–205. [PubMed] [Google Scholar]
  17. Husbands D. R. The distribution of lipoprotein lipase in tissues of the domestic fowl and the effects of feeding and starving. Br Poult Sci. 1972 Jan;13(1):85–90. doi: 10.1080/00071667208415919. [DOI] [PubMed] [Google Scholar]
  18. Jones A. L., Price J. M. Some methods of electron microscopic visualization of lipoproteins in plasma and chyle. J Histochem Cytochem. 1968 May;16(5):366–370. doi: 10.1177/16.5.366. [DOI] [PubMed] [Google Scholar]
  19. Kompiang I. P., Bensadoun A., Yang M. W. Effect of an anti-lipoprotein lipase serum on plasma triglyceride removal. J Lipid Res. 1976 Sep;17(5):498–505. [PubMed] [Google Scholar]
  20. Krumins S. A., Roth T. F. High-affinity binding of lower-density lipoproteins to chicken oocyte membranes. Biochem J. 1981 May 15;196(2):481–488. doi: 10.1042/bj1960481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kudzma D. J., Swaney J. B., Ellis E. N. Effects of estrogen administration on the lipoproteins and apoproteins of the chicken. Biochim Biophys Acta. 1979 Feb 26;572(2):257–268. doi: 10.1016/0005-2760(79)90041-9. [DOI] [PubMed] [Google Scholar]
  22. Laurell S., Tibbling G. Colorimetric micro-determination of free fatty acids in plasma. Clin Chim Acta. 1967 Apr;16(1):57–62. doi: 10.1016/0009-8981(67)90269-0. [DOI] [PubMed] [Google Scholar]
  23. Nestruck A. C., Rubinstein D. The synthesis of apoproteins of very low density lipoproteins isolated from the Golgi apparatus of rat liver. Can J Biochem. 1976 Jul;54(7):617–628. doi: 10.1139/o76-091. [DOI] [PubMed] [Google Scholar]
  24. Perry M. M., Gilbert A. B. Yolk transport in the ovarian follicle of the hen (Gallus domesticus): lipoprotein-like particles at the periphery of the oocyte in the rapid growth phase. J Cell Sci. 1979 Oct;39:257–272. doi: 10.1242/jcs.39.1.257. [DOI] [PubMed] [Google Scholar]
  25. Scanu A. M., Edelstein C. Solubility in aqueous solutions of ethanol of the small molecular weight peptides of the serum very low density and high density lipoproteins: relevance to the recovery problem during delipidation of serum lipoproteins. Anal Biochem. 1971 Dec;44(2):576–588. doi: 10.1016/0003-2697(71)90247-8. [DOI] [PubMed] [Google Scholar]
  26. Williams D. L. Apoproteins of avian very low density lipoprotein: demonstration of a single high molecular weight apoprotein. Biochemistry. 1979 Mar 20;18(6):1056–1063. doi: 10.1021/bi00573a019. [DOI] [PubMed] [Google Scholar]
  27. Wiskocil R., Bensky P., Dower W., Goldberger R. F., Gordon J. I., Deeley R. G. Coordinate regulation of two estrogen-dependent genes in avian liver. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4474–4478. doi: 10.1073/pnas.77.8.4474. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES