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. 1997 May 15;501(Pt 1):59–66. doi: 10.1111/j.1469-7793.1997.059bo.x

Activation of a novel non-selective cation channel by alloxan and H2O2 in the rat insulin-secreting cell line CRI-G1.

P S Herson 1, M L Ashford 1
PMCID: PMC1159504  PMID: 9174994

Abstract

1. Alloxan and its auto-oxidation product hydrogen peroxide (H2O2) irreversibly depolarize insulinoma cells by opening a non-selective cation channel. The channel opened is characterized by a linear current-voltage relation with a conductance of approximately 70 pS and very slow kinetics (of the order of seconds). 2. Cells are protected against the alloxan-induced channel opening and consequent cell depolarization by the presence of H2O2 and hydroxyl radical scavengers. 3. The free radical-activated non-selective cation channel is not operative in isolated patches but can be activated by the application of beta-NAD+ to the cytoplasmic aspect of the membrane.

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Selected References

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