Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1997 Nov 1;504(Pt 3):721–726. doi: 10.1111/j.1469-7793.1997.721bd.x

Extracellular glucose turnover in the striatum of unanaesthetized rats measured by quantitative microdialysis.

A E Fray 1, M Boutelle 1, M Fillenz 1
PMCID: PMC1159973  PMID: 9401977

Abstract

1. Steady-state and time-resolved quantitative microdialysis was used to measure dialysate concentration, extracellular concentration and the in vivo recovery of glucose in rat striatum. 2. The extracellular concentration of glucose, determined by the zero net flux method of Lönnroth, was 350 +/- 20 microM and the in vivo recovery was 39 +/- 2%. 3. Veratridine caused a steep decrease in dialysate glucose after an initial delay of 7.5 min. When steady-state glucose levels had been reached in the presence of veratridine the extracellular concentration was reduced to zero, but there was no significant change in in vivo recovery. 4. Measurement of the dynamic changes during the administration of veratridine showed an immediate decrease in extracellular glucose concentration and a steep rise in in vivo recovery, which accounted for the delay in the delay in the decrease in dialysate glucose. When extracellular concentration reached zero, in vivo recovery returned to control levels.

Full text

PDF
725

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boutelle M. G., Fellows L. K., Cook C. Enzyme packed bed system for the on-line measurement of glucose, glutamate, and lactate in brain microdialysate. Anal Chem. 1992 Sep 1;64(17):1790–1794. doi: 10.1021/ac00041a010. [DOI] [PubMed] [Google Scholar]
  2. Boutelle M. G., Fillenz M. Clinical microdialysis: the role of on-line measurement and quantitative microdialysis. Acta Neurochir Suppl. 1996;67:13–20. doi: 10.1007/978-3-7091-6894-3_3. [DOI] [PubMed] [Google Scholar]
  3. Bungay P. M., Morrison P. F., Dedrick R. L. Steady-state theory for quantitative microdialysis of solutes and water in vivo and in vitro. Life Sci. 1990;46(2):105–119. doi: 10.1016/0024-3205(90)90043-q. [DOI] [PubMed] [Google Scholar]
  4. Dringen R., Gebhardt R., Hamprecht B. Glycogen in astrocytes: possible function as lactate supply for neighboring cells. Brain Res. 1993 Oct 1;623(2):208–214. doi: 10.1016/0006-8993(93)91429-v. [DOI] [PubMed] [Google Scholar]
  5. Fellows L. K., Boutelle M. G., Fillenz M. Extracellular brain glucose levels reflect local neuronal activity: a microdialysis study in awake, freely moving rats. J Neurochem. 1992 Dec;59(6):2141–2147. doi: 10.1111/j.1471-4159.1992.tb10105.x. [DOI] [PubMed] [Google Scholar]
  6. Forsyth R., Fray A., Boutelle M., Fillenz M., Middleditch C., Burchell A. A role for astrocytes in glucose delivery to neurons? Dev Neurosci. 1996;18(5-6):360–370. doi: 10.1159/000111429. [DOI] [PubMed] [Google Scholar]
  7. Fray A. E., Forsyth R. J., Boutelle M. G., Fillenz M. The mechanisms controlling physiologically stimulated changes in rat brain glucose and lactate: a microdialysis study. J Physiol. 1996 Oct 1;496(Pt 1):49–57. doi: 10.1113/jphysiol.1996.sp021664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Justice J. B., Jr Quantitative microdialysis of neurotransmitters. J Neurosci Methods. 1993 Jul;48(3):263–276. doi: 10.1016/0165-0270(93)90097-b. [DOI] [PubMed] [Google Scholar]
  9. Lönnroth P., Jansson P. A., Smith U. A microdialysis method allowing characterization of intercellular water space in humans. Am J Physiol. 1987 Aug;253(2 Pt 1):E228–E231. doi: 10.1152/ajpendo.1987.253.2.E228. [DOI] [PubMed] [Google Scholar]
  10. Magistretti P. J., Pellerin L. Cellular bases of brain energy metabolism and their relevance to functional brain imaging: evidence for a prominent role of astrocytes. Cereb Cortex. 1996 Jan-Feb;6(1):50–61. doi: 10.1093/cercor/6.1.50. [DOI] [PubMed] [Google Scholar]
  11. Morrison P. F., Bungay P. M., Hsiao J. K., Ball B. A., Mefford I. N., Dedrick R. L. Quantitative microdialysis: analysis of transients and application to pharmacokinetics in brain. J Neurochem. 1991 Jul;57(1):103–119. doi: 10.1111/j.1471-4159.1991.tb02105.x. [DOI] [PubMed] [Google Scholar]
  12. Morton D. B., Griffiths P. H. Guidelines on the recognition of pain, distress and discomfort in experimental animals and an hypothesis for assessment. Vet Rec. 1985 Apr 20;116(16):431–436. doi: 10.1136/vr.116.16.431. [DOI] [PubMed] [Google Scholar]
  13. Pardridge W. M. Brain metabolism: a perspective from the blood-brain barrier. Physiol Rev. 1983 Oct;63(4):1481–1535. doi: 10.1152/physrev.1983.63.4.1481. [DOI] [PubMed] [Google Scholar]
  14. Ronne-Engström E., Carlson H., Liu Y., Ungerstedt U., Hillered L. Influence of perfusate glucose concentration on dialysate lactate, pyruvate, aspartate, and glutamate levels under basal and hypoxic conditions: a microdialysis study in rat brain. J Neurochem. 1995 Jul;65(1):257–262. doi: 10.1046/j.1471-4159.1995.65010257.x. [DOI] [PubMed] [Google Scholar]
  15. Silver I. A., Erecińska M. Extracellular glucose concentration in mammalian brain: continuous monitoring of changes during increased neuronal activity and upon limitation in oxygen supply in normo-, hypo-, and hyperglycemic animals. J Neurosci. 1994 Aug;14(8):5068–5076. doi: 10.1523/JNEUROSCI.14-08-05068.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sokoloff L. The brain as a chemical machine. Prog Brain Res. 1992;94:19–33. doi: 10.1016/s0079-6123(08)61736-7. [DOI] [PubMed] [Google Scholar]
  17. Whitesell R. R., Ward M., McCall A. L., Granner D. K., May J. M. Coupled glucose transport and metabolism in cultured neuronal cells: determination of the rate-limiting step. J Cereb Blood Flow Metab. 1995 Sep;15(5):814–826. doi: 10.1038/jcbfm.1995.102. [DOI] [PubMed] [Google Scholar]
  18. de Boer P., Damsma G., Fibiger H. C., Timmerman W., de Vries J. B., Westerink B. H. Dopaminergic-cholinergic interactions in the striatum: the critical significance of calcium concentrations in brain microdialysis. Naunyn Schmiedebergs Arch Pharmacol. 1990 Nov;342(5):528–534. doi: 10.1007/BF00169041. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES