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. 1979 Jul 1;181(1):245–246. doi: 10.1042/bj1810245

Localization of the heparin-releasable lipase in situ in the rat liver.

T Kuusi, E A Nikklä, I Virtanen, P K Kinnunen
PMCID: PMC1161147  PMID: 384999

Abstract

Immunofluorescence and immuno-electron microscopy were used for the localization of the heparin-releasable lipase in situ in the rat liver. The lipase is located exclusively on the liver endothelial cells. No labelling could be detected on the parenchymal of Kupffer cells, or in the livers of heparin-pretreated animals. The physiological significance of the endothelial localization of the hepatic lipase is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen J. M., Nervi F. O., Dietschy J. M. Rate constants for the uptake of cholesterol from various intestinal and serum lipoprotein fractions by the liver of the rat in vivo. Biochim Biophys Acta. 1977 Feb 23;486(2):298–307. doi: 10.1016/0005-2760(77)90025-x. [DOI] [PubMed] [Google Scholar]
  2. Assmann G., Krauss R. M., Fredrickson D. S., Levy R. I. Characterization, subcellular localization, and partial purification of a heparin-released triglyceride lipase from rat liver. J Biol Chem. 1973 Mar 25;248(6):1992–1999. [PubMed] [Google Scholar]
  3. El-Maghrabi M. R., Waite M., Rudel L. L., Sisson P. Hydrolysis of monoacylglycerol in lipoprotein remnants catalyzed by the liver plasma membrane monoacylglycerol acyltransferase. J Biol Chem. 1978 Feb 10;253(3):974–981. [PubMed] [Google Scholar]
  4. Felts J. M., Mayes P. A. Release of lipoprotein lipase from the perfused liver of the rat. Nature. 1967 May 6;214(5088):620–621. doi: 10.1038/214620a0. [DOI] [PubMed] [Google Scholar]
  5. Glomset J. A. The plasma lecithins:cholesterol acyltransferase reaction. J Lipid Res. 1968 Mar;9(2):155–167. [PubMed] [Google Scholar]
  6. Huttunen J. K., Ehnholm C., Kekki M., Nikkilä E. A. Post-heparin plasma lipoprotein lipase and hepatic lipase in normal subjects and in patients with hypertriglyceridaemia: correlations to sex, age and various parameters of triglyceride metabolism. Clin Sci Mol Med. 1976 Apr;50(4):249–260. doi: 10.1042/cs0500249. [DOI] [PubMed] [Google Scholar]
  7. Jansen H., van Berkel T. J., Hülsmann W. C. Binding of liver lipase to parenchymal and non-parenchymal rat liver cells. Biochem Biophys Res Commun. 1978 Nov 14;85(1):148–152. doi: 10.1016/s0006-291x(78)80022-9. [DOI] [PubMed] [Google Scholar]
  8. Kuusi T., Kinnunen P. K., Ehnholm C., Nikkilä E. A. A simple purification procedure for rat hepatic lipase. FEBS Lett. 1979 Feb 15;98(2):314–318. doi: 10.1016/0014-5793(79)80207-0. [DOI] [PubMed] [Google Scholar]
  9. LaRosa J. C., Levy R. I., Windmueller H. G., Fredrickson D. S. Comparison of the triglyceride lipase of liver, adipose tissue, and postheparin plasma. J Lipid Res. 1972 May;13(3):356–363. [PubMed] [Google Scholar]
  10. Stein O., Rachmilewitz D., Sanger L., Eisenberg S., Stein Y. Metabolism of iodinated very low density lipoprotein in the rat. Autoradiographic localization in the liver. Biochim Biophys Acta. 1974 Aug 22;360(2):205–216. doi: 10.1016/0005-2760(74)90170-2. [DOI] [PubMed] [Google Scholar]
  11. Stein O., Stein Y., Goodman D. S., Fidge N. H. The metabolism of chylomicron cholesteryl ester in rat liver. A combined radioautographic-electron microscopic and biochemical study. J Cell Biol. 1969 Dec;43(3):410–431. doi: 10.1083/jcb.43.3.410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Van Berkel T. J., Koster J. F., Hülsmann W. C. High density lipoprotein and low density lipoprotein catabolism by human liver and parenchymal and non-parenchymal cells from rat liver. Biochim Biophys Acta. 1977 Mar 25;486(3):586–589. doi: 10.1016/0005-2760(77)90114-x. [DOI] [PubMed] [Google Scholar]
  13. Waite M., Sisson P. Solubilization by heparin of the phospholipase A1 from the plasma membranes of rat liver. J Biol Chem. 1973 Oct 25;248(20):7201–7206. [PubMed] [Google Scholar]
  14. Weibel E. R., Stäubli W., Gnägi H. R., Hess F. A. Correlated morphometric and biochemical studies on the liver cell. I. Morphometric model, stereologic methods, and normal morphometric data for rat liver. J Cell Biol. 1969 Jul;42(1):68–91. doi: 10.1083/jcb.42.1.68. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. van Berkel T. J., van Tol A. In vivo uptake of human and rat low density and high density lipoprotein by parenchymal and nonparenchymal cells from rat liver. Biochim Biophys Acta. 1978 Aug 25;530(2):299–304. doi: 10.1016/0005-2760(78)90015-2. [DOI] [PubMed] [Google Scholar]

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