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. 1979 Jul 1;181(1):251–253. doi: 10.1042/bj1810251

Dansylation of human serum albumin in the study of the primary binding sites of bilirubin and L-tryptophan.

C Jacobsen, J Jacobsen
PMCID: PMC1161149  PMID: 486157

Abstract

Binding of bilirubin and of L-tryptophan to dansylated albumins was investigated. Dansylation of less than one lysine residue per molecule of albumin did not affect the bilirubin binding, but decreased the L-tryptophan binding, indicating that dansylation had taken place in or near the l-tryptophan-binding site. Native albumin and albumin-bilirubin 1:1 complex showed the same affinity for L-tryptophan. The results indicate that, although L-tryptophan and bilirubin are bound in the same region, perhaps in a common cavity of the albumin molecule, such a cavity is sufficiently large to contain both ligands.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chen R. F. Dansyl labeled proteins: determination of extinction coefficienc and number of bound residues with radioactive dansyl chloride. Anal Biochem. 1968 Oct 24;25(1):412–416. doi: 10.1016/0003-2697(68)90116-4. [DOI] [PubMed] [Google Scholar]
  2. Chen R. F. Removal of fatty acids from serum albumin by charcoal treatment. J Biol Chem. 1967 Jan 25;242(2):173–181. [PubMed] [Google Scholar]
  3. Gambhir K. K., McMenamy R. H., Watson F. Positions in human serum albumin which involve the indole binding site. Sequence of 107-residue fragment. J Biol Chem. 1975 Sep 10;250(17):6711–6719. [PubMed] [Google Scholar]
  4. Jacobsen C. Chemical modification of the high-affinity bilirubin-binding site of human-serum albumin. Eur J Biochem. 1972 Jun 9;27(3):513–519. doi: 10.1111/j.1432-1033.1972.tb01867.x. [DOI] [PubMed] [Google Scholar]
  5. Jacobsen C. Lysine residue 240 of human serum albumin is involved in high-affinity binding of bilirubin. Biochem J. 1978 May 1;171(2):453–459. doi: 10.1042/bj1710453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jacobsen C. Trinitrophenylation of the bilirubin binding site of human serum albumin. Int J Pept Protein Res. 1975;7(2):161–165. doi: 10.1111/j.1399-3011.1975.tb02427.x. [DOI] [PubMed] [Google Scholar]
  7. Jacobsen J. Binding of bilirubin to human serum albumin - determination of the dissociation constants. FEBS Lett. 1969 Oct 21;5(2):112–114. doi: 10.1016/0014-5793(69)80307-8. [DOI] [PubMed] [Google Scholar]
  8. Kragh-Hansen U., Moller J. V., Sheikh M. I. A spectrophotometric micromethod for the determination of binding of phenol red to plasma proteins of various species. Pflugers Arch. 1972;337(2):163–176. doi: 10.1007/BF00587838. [DOI] [PubMed] [Google Scholar]
  9. Meloun B., Morávek L., Kostka V. Complete amino acid sequence of human serum albumin. FEBS Lett. 1975 Oct 15;58(1):134–137. doi: 10.1016/0014-5793(75)80242-0. [DOI] [PubMed] [Google Scholar]
  10. Sjöholm I., Ljungstedt I. Studies on the tryptophan and drug-binding properties of human serum albumin fragments by affinity chromatography and circular dichroism measurements. J Biol Chem. 1973 Dec 25;248(24):8434–8441. [PubMed] [Google Scholar]
  11. Walker J. E. Lysine residue 199 of human serum albumin is modified by acetylsalicyclic acid. FEBS Lett. 1976 Jul 15;66(2):173–175. doi: 10.1016/0014-5793(76)80496-6. [DOI] [PubMed] [Google Scholar]

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