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. 1979 Sep 1;181(3):659–665. doi: 10.1042/bj1810659

The isolation and partial sequence of peptides produced by cyanogen bromide cleavage of calf thymus non-histone chromosomal high-mobility-group protein 2. Sequence homology with non-histone chromosomal high-mobility-group protein 1.

J M Walker, K Gooderham, E W Johns
PMCID: PMC1161205  PMID: 518545

Abstract

Peptides produced by CNBr cleavage of non-histone chromosomal protein HMG 2 (CNBr peptides) were isolated and characterized, and their partial sequences were determined. The present sequence data account for over half of the sequence of the protein HMG (high-mobility-group) 2 molecule, and, together with previously published results, provide interesting information on the charge distribution within the molecule. Comparison of the CNBr-peptide-sequence data for protein HMG 2 with the previously published data on the CNBr peptides from protein HMG 1 reveals extensive sequence homology between the two proteins. Detailed evidence for the amino acid-sequence data has been deposited as Supplementary Publication SUP 50095 (6 pages) at the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies may be obtained on the terms given in Biochem. J. (1978) 169, 5.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Goodwin G. H., Nicolas R. H., Johns E. W. An improved large scale fractionation of high mobility group non-histone chromatin proteins. Biochim Biophys Acta. 1975 Oct 20;405(2):280–291. doi: 10.1016/0005-2795(75)90094-x. [DOI] [PubMed] [Google Scholar]
  2. Goodwin G. H., Rabbani A., Nicolas P. H., Johns E. W. The isolation of the high mobility group non-histone chromosomal protein HMG 14. FEBS Lett. 1977 Aug 15;80(2):413–416. doi: 10.1016/0014-5793(77)80488-2. [DOI] [PubMed] [Google Scholar]
  3. Goodwin G. H., Woodhead L., Johns E. W. The presence of high mobility group non-histone chromatin proteins in isolated nucleosomes. FEBS Lett. 1977 Jan 15;73(1):85–88. [PubMed] [Google Scholar]
  4. Hartley B. S. Strategy and tactics in protein chemistry. Biochem J. 1970 Oct;119(5):805–822. doi: 10.1042/bj1190805f. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kinkade J. M., Jr, Cole R. D. A structural comparison of different lysine-rich histones of calf thymus. J Biol Chem. 1966 Dec 25;241(24):5798–5805. [PubMed] [Google Scholar]
  6. Kinkade J. M., Jr, Cole R. D. The resolution of four lysine-rich histones derived from calf thymus. J Biol Chem. 1966 Dec 25;241(24):5790–5797. [PubMed] [Google Scholar]
  7. Kornberg R. D. Chromatin structure: a repeating unit of histones and DNA. Science. 1974 May 24;184(4139):868–871. doi: 10.1126/science.184.4139.868. [DOI] [PubMed] [Google Scholar]
  8. Piszkiewicz D., Landon M., Smith E. L. Anomalous cleavage of aspartyl-proline peptide bonds during amino acid sequence determinations. Biochem Biophys Res Commun. 1970 Sep 10;40(5):1173–1178. doi: 10.1016/0006-291x(70)90918-6. [DOI] [PubMed] [Google Scholar]
  9. Rabbani A., Goodwin G. H., Johns E. W. High mobility group non-histone chromosomal proteins from chicken erythrocytes. Biochem Biophys Res Commun. 1978 Mar 30;81(2):351–358. doi: 10.1016/0006-291x(78)91540-1. [DOI] [PubMed] [Google Scholar]
  10. Shaw B. R., Herman T. M., Kovacic R. T., Beaudreau G. S., Van Holde K. E. Analysis of subunit organization in chicken erythrocyte chromatin. Proc Natl Acad Sci U S A. 1976 Feb;73(2):505–509. doi: 10.1073/pnas.73.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Spiker S., Mardian J. K., Isenberg I. Chomosomal HMG proteins occur in three eukaryotic kingdoms. Biochem Biophys Res Commun. 1978 May 15;82(1):129–135. doi: 10.1016/0006-291x(78)90586-7. [DOI] [PubMed] [Google Scholar]
  12. Sterner R., Boffa L. C., Vidali G. Comparative structural analysis of high mobility group proteins from a variety of sources. Evidence for a high mobility group protein unique to avian erythrocyte nuclei. J Biol Chem. 1978 Jun 10;253(11):3830–3836. [PubMed] [Google Scholar]
  13. Varshavsky A. J., Bakayev V. V., Georgiev G. P. Heterogeneity of chromatin subunits in vitro and location of histone H1. Nucleic Acids Res. 1976 Feb;3(2):477–492. doi: 10.1093/nar/3.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Walker J. M., Goodwin G. H., Johns E. W. The primary structure of the nucleosome-associated chromosomal protein HMG 14. FEBS Lett. 1979 Apr 15;100(2):394–398. doi: 10.1016/0014-5793(79)80378-6. [DOI] [PubMed] [Google Scholar]
  15. Walker J. M., Goodwin G. H., Johns E. W. The similarity between the primary structures of two non-histone chromosomal proteins. Eur J Biochem. 1976 Mar 1;62(3):461–469. doi: 10.1111/j.1432-1033.1976.tb10179.x. [DOI] [PubMed] [Google Scholar]
  16. Walker J. M., Hastings J. R., Johns E. W. A novel continuous sequence of 41 aspartic and glutamic residues in a non-histone chromosomal protein. Nature. 1978 Jan 19;271(5642):281–282. doi: 10.1038/271281a0. [DOI] [PubMed] [Google Scholar]
  17. Walker J. M., Hastings J. R., Johns E. W. The primary structure of a non-histone chromosomal protein. Eur J Biochem. 1977 Jun 15;76(2):461–468. doi: 10.1111/j.1432-1033.1977.tb11616.x. [DOI] [PubMed] [Google Scholar]
  18. Walker J. M., Parker B. M., Johns E. W. Isolation and partial sequence of the cyanogen bromide peptides from calf thymus non-histone chromosomal protein HMG 1. Int J Pept Protein Res. 1978 Nov;12(5):269–276. doi: 10.1111/j.1399-3011.1978.tb02897.x. [DOI] [PubMed] [Google Scholar]
  19. Watson D. C., Peters E. H., Dixon G. H. The purification, characterization and partial sequence determination of a trout testis non-histone protein, HMG-T. Eur J Biochem. 1977 Mar 15;74(1):53–60. doi: 10.1111/j.1432-1033.1977.tb11365.x. [DOI] [PubMed] [Google Scholar]

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