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. 1979 Aug 15;182(2):421–427. doi: 10.1042/bj1820421

Role of insulin receptors in the changing metabolism of adipose tissue during pregnancy and lactation in the rat.

D J Flint, P A Sinnett-Smith, R A Clegg, R G Vernon
PMCID: PMC1161323  PMID: 508293

Abstract

Changes in the volume, the rates of fatty acid synthesis and synthesis of the glycerol moiety of acylglycerols, the activity of lipoprotein lipase, and the number and affinity of insulin receptors of adipocytes, and concentrations of serum insulin, prolactin and progesterone were determined in virgin rats and in rats at various stages of pregnancy and lactation. Changes in the metabolic activities of adipose tissue appeared to be synchronized and primarily comprised a marked decrease in anabolic activity around parturition. In contrast, the number of insulin receptors (Kd 1.5 nM) per adipocyte doubled during pregnancy before returning to normal values around parturition. It is postulated that the increase in the number of insulin receptors is an adaptation to counteract the effects of insulin-antagonistic hormones during pregnancy and that the decrease in the number of receptors is primarily responsible for the loss of anabolic activity around parturition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEATON G. H., BEARE J., RYU M. H., McHENRY E. W. Protein metabolism in the pregnant rat. J Nutr. 1954 Oct 11;54(2):291–304. doi: 10.1093/jn/54.2.291. [DOI] [PubMed] [Google Scholar]
  2. Bourne A. R., Richardson D. P., Bruckdorfer K. R., Yudkin J. Some effects of different dietary carbohydrates on pregnancy and lactation in rats. Nutr Metab. 1975;19(1-2):73–90. doi: 10.1159/000175649. [DOI] [PubMed] [Google Scholar]
  3. Demetriou J. A., Austin F. G. A rapid competitive protein-binding assay for plasma progesterone. Clin Chim Acta. 1971 Jun;33(1):21–32. doi: 10.1016/0009-8981(71)90244-0. [DOI] [PubMed] [Google Scholar]
  4. DiGirolamo M., Howe M. D., Esposito J., Thurman L., Owens J. L. Metabolic patterns and insulin responsiveness of enlarging fat cells. J Lipid Res. 1974 Jul;15(4):332–338. [PubMed] [Google Scholar]
  5. Elias J. J., Pitelka D. R., Armstrong R. C. Changes in fat cell morphology during lactation in the mouse. Anat Rec. 1973 Dec;177(4):533–547. doi: 10.1002/ar.1091770407. [DOI] [PubMed] [Google Scholar]
  6. Freychet P., Kahn R., Roth J., Neville D. M., Jr Insulin interactions with liver plasma membranes. Independence of binding of the hormone and its degradation. J Biol Chem. 1972 Jun 25;247(12):3953–3961. [PubMed] [Google Scholar]
  7. Gavin J. R., 3rd, Roth J., Neville D. M., Jr, de Meyts P., Buell D. N. Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture. Proc Natl Acad Sci U S A. 1974 Jan;71(1):84–88. doi: 10.1073/pnas.71.1.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gliemann J., Osterlind K., Vinten J., Gammeltoft S. A procedure for measurement of distribution spaces in isolated fat cells. Biochim Biophys Acta. 1972 Nov 24;286(1):1–9. doi: 10.1016/0304-4165(72)90082-7. [DOI] [PubMed] [Google Scholar]
  9. Hamosh M., Clary T. R., Chernick S. S., Scow R. O. Lipoprotein lipase activity of adipose and mammary tissue and plasma triglyceride in pregnant and lactating rats. Biochim Biophys Acta. 1970 Sep 8;210(3):473–482. doi: 10.1016/0005-2760(70)90044-5. [DOI] [PubMed] [Google Scholar]
  10. Hanson R. W., Ballard F. J. Citrate, pyruvate, and lactate contaminants of commercial serum albumin. J Lipid Res. 1968 Sep;9(5):667–668. [PubMed] [Google Scholar]
  11. Ip C., Tepperman H. M., Holohan P., Tepperman J. Insulin binding and insulin response of adipocytes from rats adapted to fat feeding. J Lipid Res. 1976 Nov;17(6):588–599. [PubMed] [Google Scholar]
  12. Kasuga M., Akanuma Y., Iwamoto Y., Kosaka K. Effects of fasting and refeeding of insulin receptors and glucose metabolism in rat adipocytes. Endocrinology. 1977 May;100(5):1384–1390. doi: 10.1210/endo-100-5-1384. [DOI] [PubMed] [Google Scholar]
  13. Knopp R. H., Herrera E., Freinkel N. Carbohydrate metabolism in pregnancy. 8. Metabolism of adipose tissue isolated from fed and fasted pregnant rats during late gestation. J Clin Invest. 1970 Jul;49(7):1438–1446. doi: 10.1172/JCI106361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Knopp R. H., Ruder H. J., Herrera E., Freinkel N. Carbohydrate metabolism in pregnancy. VII. Insulin tolerance during late pregnancy in the fed and fasted rat. Acta Endocrinol (Copenh) 1970 Oct;65(2):352–360. [PubMed] [Google Scholar]
  15. Knopp R. H., Saudek C. D., Arky R. A., O'Sullivan J. B. 2 phases of adipose tissue metabolism in pregnancy: maternal adaptations for fetal growth. Endocrinology. 1973 Apr;92(4):984–988. doi: 10.1210/endo-92-4-984. [DOI] [PubMed] [Google Scholar]
  16. Nilsson-Ehle P., Schotz M. C. A stable, radioactive substrate emulsion for assay of lipoprotein lipase. J Lipid Res. 1976 Sep;17(5):536–541. [PubMed] [Google Scholar]
  17. Olefsky J. M., Reaven G. M. Effects of age and obesity on insulin binding to isolated adipocytes. Endocrinology. 1975 Jun;96(6):1486–1498. doi: 10.1210/endo-96-6-1486. [DOI] [PubMed] [Google Scholar]
  18. Otway S., Robinson D. S. The significance of changes in tissue clearing-factor lipase activity in relation to the lipaemia of pregnancy. Biochem J. 1968 Feb;106(3):677–682. doi: 10.1042/bj1060677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Robinson A. M., Girard J. R., Williamson D. H. Evidence for a role of insulin in the regulation of lipogenesis in lactating rat mammary gland. Measurements of lipogenesis in vivo and plasma hormone concentrations in response to starvation and refeeding. Biochem J. 1978 Oct 15;176(1):343–346. doi: 10.1042/bj1760343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SPRAY C. M. A study of some aspects of reproduction by means of chemical analysis. Br J Nutr. 1950;4(4):354–360. doi: 10.1079/bjn19500059. [DOI] [PubMed] [Google Scholar]
  21. Salans L. B., Dougherty J. W. The effect of insulin upon glucose metabolism by adipose cells of different size. Influence of cell lipid and protein content, age, and nutritional state. J Clin Invest. 1971 Jul;50(7):1399–1410. doi: 10.1172/JCI106623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Smith R. W. The effects of pregnancy, lactation and involution on the metabolism of glucose by rat parametrial adipose tissue. J Dairy Res. 1973 Oct;40(3):353–360. doi: 10.1017/s0022029900014722. [DOI] [PubMed] [Google Scholar]
  23. Sutter-Dub M. T., Leclercq R., Sutter B. C., Jacquot R. Plasma glucose, progesterone and immunoreactive insulin levels in the lactating rat. Horm Metab Res. 1974 Jul;6(4):297–300. doi: 10.1055/s-0028-1093852. [DOI] [PubMed] [Google Scholar]
  24. Tyson J. E., Felig P. Medical aspects of diabetes in pregnancy and the diabetogenic effects of oral contraceptives. Med Clin North Am. 1971 Jul;55(4):947–959. doi: 10.1016/s0025-7125(16)32489-0. [DOI] [PubMed] [Google Scholar]
  25. Vernon R. G. Development of perirenal adipose tissue in the neonatal lamb: effects of dietary safflower oil. Biol Neonate. 1977;32(1-2):15–23. doi: 10.1159/000240991. [DOI] [PubMed] [Google Scholar]
  26. Wang C., Smith R. L. Lowry determination of protein in the presence of Triton X-100. Anal Biochem. 1975 Feb;63(2):414–417. doi: 10.1016/0003-2697(75)90363-2. [DOI] [PubMed] [Google Scholar]
  27. Zinder O., Hamosh M., Fleck T. R., Scow R. O. Effect of prolactin on lipoprotein lipase in mammary glands and adipose tissue of rats. Am J Physiol. 1974 Mar;226(3):742–748. doi: 10.1152/ajplegacy.1974.226.3.744. [DOI] [PubMed] [Google Scholar]

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