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. 1980 Feb 1;185(2):387–395. doi: 10.1042/bj1850387

Sequence of the N-terminal half of Bacillus amyloliquefaciens alpha-amylase.

H Chung, F Friedberg
PMCID: PMC1161365  PMID: 6156671

Abstract

Bacillus amyloliquefaciens alpha-amylase (1,4-alpha-D-glucan glucanohydrolase. EC 3.2.1.1), which is commercially supplied as 'Bacillus subtilis alpha-amylase' does not cross-react immunologically with B. subtilis alpha-amylase. This enzyme (from B. amyloliquefaciens) was cleaved by treatment with CNBr into seven fragments. Peptide A was selected for sequence determination. It is the longest one, containing 185 amino acids (i.e. approx. 50% of the total molecule) and connects to the hexapeptide of the N-terminus. Its primary structure was aligned by use of various proteolytic enzymes. The sequence of amino acids 181-184 is identical with that of amino acids 14-17 of the alpha-amylase isolated from B. subtilis (except that amino acid 183 is asparagine rather than aspartic acid).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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