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. 1979 Dec 1;183(3):555–559. doi: 10.1042/bj1830555

A new fluorogenic substrate for plasmin.

P A Pierzchala, C P Dorn, M Zimmerman
PMCID: PMC1161636  PMID: 161507

Abstract

A new fluorogenic peptide substrate for plasmin, 7-(N-succinoylalanylphenylalanyl-lysylamido)-4-methylcoumarin trifluoroacetate salt, was prepared that can be used in a simple and direct assay. The results obtained by the assay method are linear over a wide range of enzyme concentrations and sensitive enough to detect as little as 10(-5) CTA units of plasmin. By making use of the inhibitor Trasylol and the differences in kinetic constants, plasmin can be specifically assayed even in the presence of the plasminogen activator thrombin, as well as in culture fluids from HeLa cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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