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. 1979 Dec 1;183(3):647–656. doi: 10.1042/bj1830647

Purification of pig synovial collagenase to high specific activity.

T E Cawston, J A Tyler
PMCID: PMC1161646  PMID: 231970

Abstract

1. Pig synovium in tissue culture secretes a specific collagenase in a latent form. 2. The latent enzyme was concentrated by (NH4)2SO4 precipitation and activated with 4-aminophenylmercuric acetate, and the active enzyme was purified by chromatography on Ultrogel AcA44, DEAE-cellulose, heparin-Sepharose and a zinc-chelate medium to a specific activity of 53 400 units/mg. of protein. 3. The enzyme was shown to be essentially homogeneous by polyacrylamide-gel electrophoresis. 4. The purified collagenase digested collagen to give the characteristic three-quarter and one-quarter pieces.

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Selected References

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