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. 1980 Mar 15;186(3):679–685. doi: 10.1042/bj1860679

A species-non-specific liver plasma-membrane antigen and its involvement in chronic active hepatitis.

T A De Kretser, I G McFarlane, A L Eddleston, R Williams
PMCID: PMC1161702  PMID: 6772154

Abstract

Rabbit liver plasma membranes were isolated and purified by using an aqueous two-phase polymer system. Examination of these preparations with respect to electron-microscopical appearance, distribution of marker enzymes and gross biochemical composition revealed them to be free from contamination by intracellular components. Sera from ten patients with chronic active hepatitis, four with and six without hepatitis B viral markers (HBsAg) in their sera, produced a single precipitin line on immunodiffusion against a detergent extract of the isolated plasma membranes. Sera from HBsAg-positive and HBsAg-negative patients reacted against the same antigen. This antigen was enriched in the plasma membrane preparations compared with whole-liver homogenates and was identical with a species-non-specific antigen in a macromolecular fraction of normal human liver, which has been previously described as liver-specific lipoprotein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blitzer B. L., Boyer J. L. Cytochemical localization of Na+, K+-ATPase in the rat hepatocyte. J Clin Invest. 1978 Nov;62(5):1104–1108. doi: 10.1172/JCI109216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cochrane A. M., Moussouros A., Thomsom A. D., Eddleston A. L., Wiiliams R. Antibody-dependent cell-mediated (K cell) cytotoxicity against isolated hepatocytes in chronic active hepatitis. Lancet. 1976 Feb 28;1(7957):441–444. doi: 10.1016/s0140-6736(76)91472-0. [DOI] [PubMed] [Google Scholar]
  3. Dienstag J. L., Isselbacher K. J. Liver-specific protein: more questions than answers. N Engl J Med. 1978 Jul 6;299(1):40–42. doi: 10.1056/NEJM197807062990110. [DOI] [PubMed] [Google Scholar]
  4. EMMELOT P., BOS C. J., BENEDETTI E. L., RUEMKE P. STUDIES ON PLASMA MEMBRANES. I. CHEMICAL COMPOSITION AND ENZYME CONTENT OF PLASMA MEMBRANES ISOLATED FROM RAT LIVER. Biochim Biophys Acta. 1964 Jul 15;90:126–145. doi: 10.1016/0304-4165(64)90125-4. [DOI] [PubMed] [Google Scholar]
  5. Frings C. S., Dunn R. T. A colorimetric method for determination of total serum lipids based on the sulfo-phospho-vanillin reaction. Am J Clin Pathol. 1970 Jan;53(1):89–91. doi: 10.1093/ajcp/53.1.89. [DOI] [PubMed] [Google Scholar]
  6. Hess H. H., Derr J. E. Assay of inorganic and organic phosphorus in the 0.1-5 nanomole range. Anal Biochem. 1975 Feb;63(2):607–613. doi: 10.1016/0003-2697(75)90388-7. [DOI] [PubMed] [Google Scholar]
  7. Hopf U., Meyer zum Büschenfelde K. H., Freudenberg J. Liver-specific antigens of different species. II. Localization of a membrane antigen at cell surface of isolated hepatocytes. Clin Exp Immunol. 1974 Jan;16(1):117–123. [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lesko L., Donlon M., Marinetti G. V., Hare J. D. A rapid method for the isolation of rat liver plasma membranes using an aqueous two-phase polymer system. Biochim Biophys Acta. 1973 Jun 22;311(2):173–179. doi: 10.1016/0005-2736(73)90264-2. [DOI] [PubMed] [Google Scholar]
  10. McFarlane I. G., Wojcicka B. M., Zucker G. M., Eddleston A. L., Williams R. Purification and characterization of human liver-specific membrane lipoprotein (LSP). Clin Exp Immunol. 1977 Mar;27(3):381–390. [PMC free article] [PubMed] [Google Scholar]
  11. Meyer zum Büschenfelde, Miescher P. A. Liver specific antigens. Purification and characterization. Clin Exp Immunol. 1972 Jan;10(1):89–102. [PMC free article] [PubMed] [Google Scholar]
  12. Miller J., Smith M. G., Mitchell C. G., Reed W. D., Eddleston A. L., Williams R. Cell-mediated immunity to a human liver-specific antigen in patients with active chronic hepatitis and primary biliary cirrhosis. Lancet. 1972 Aug 12;2(7772):296–297. doi: 10.1016/s0140-6736(72)92904-2. [DOI] [PubMed] [Google Scholar]
  13. Rudel L. L., Morris M. D. Determination of cholesterol using o-phthalaldehyde. J Lipid Res. 1973 May;14(3):364–366. [PubMed] [Google Scholar]
  14. Schnaitman C., Erwin V. G., Greenawalt J. W. The submitochondrial localization of monoamine oxidase. An enzymatic marker for the outer membrane of rat liver mitochondria. J Cell Biol. 1967 Mar;32(3):719–735. doi: 10.1083/jcb.32.3.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. TSANEV R., MARKOV G. G. Substances interfering with spectrophotometric estimation of nucleic acids and their elimination by the two-wavelength method. Biochim Biophys Acta. 1960 Aug 26;42:442–452. doi: 10.1016/0006-3002(60)90822-2. [DOI] [PubMed] [Google Scholar]
  16. Tage-Jensen U., Arnold W., Dietrichson O., Hardt F., Hopf U., Meyer Zum Büschenfelde K. H., Nielsen J. O. Liver-cell-membrane autoantibody specific for inflammatory liver diseases. Br Med J. 1977 Jan 22;1(6055):206–208. doi: 10.1136/bmj.1.6055.206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tsantoulas D. C., McFarlane I. G., Portmann B., Eddleston A. L., Williams R. Cell-mediated immunity to human Tamm-Horsfall glycoprotein in autoimmune liver disease with renal tubular acidosis. Br Med J. 1974 Nov 30;4(5943):491–494. doi: 10.1136/bmj.4.5943.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wisher M. H., Evans W. H. Functional polarity of the rat hepatocyte surface membrane. Isolation and characterization of plasma-membrane subfractions from the blood-sinusoidal, bile-Canalicular and contiguous surfaces of the hepatocyte. Biochem J. 1975 Feb;146(2):375–388. doi: 10.1042/bj1460375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. de Kretser T. A., Livett B. G. Skeletal-muscle sarcolemma from normal and dystrophic mice. Isolation, characterization and lipid composition. Biochem J. 1977 Nov 15;168(2):229–237. doi: 10.1042/bj1680229. [DOI] [PMC free article] [PubMed] [Google Scholar]

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