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. 1980 Mar 15;186(3):873–879. doi: 10.1042/bj1860873

Lipoprotein lipase activity of rat cardiac muscle. Changes in the enzyme activity during incubations of isolated cardiac-muscle cells in vitro.

P Chohan, A Cryer
PMCID: PMC1161724  PMID: 7396841

Abstract

1. Isolated cardiac-muscle cells from the hearts of adult rats were shown to retain a high amount of viability during 4 h of incubation when viability was assessed by Trypan Bue stain exclusion and intracellular enzyme leakage. 2. The cells also retained their ability to take up O2 and utilize added substrates over the period of incubation at both 25 and 30 degrees C. 3. When cells from the hearts of fed rats were incubated in a buffered-salts solution at pH 7.4 in the presence of amino acids and heparin, lipoprotein lipase activity in the medium increased progressively. 4. During these incubations the intracellular activity of the enzyme remained constant and the total activity of lipoprotein lipase in the system (cells plus medium) increased by 80% over the 4 h of incubation at 25 degrees C. 5. In the absence of heparin only low amounts of enzyme activity were detectable in the medium and the total lipoprotein lipase activity in the system remained constant. 6. The measurement of lipoprotein lipase activity in either fresh homogenates of the cells or in homogenates of acetone/diethyl ether-dried powders of the cells had no effect on the overall pattern of activity change during the incubations, although as reported previously the total activity detected with acetone/diethyl either-dried preparations was approx. 3-fold higher than with fresh cell homogenates. 7. The observations were compared with published data on lipoprotein lipase activity changes in neonatal heart cell cultures maintained in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Argüello C., De La Cruz M. V., Sánchez C. Ultrastructural and experimental evidence of myocardial cell differentiation into connective tissue cells in embryonic chick heart. J Mol Cell Cardiol. 1978 Apr;10(4):307–315. doi: 10.1016/0022-2828(78)90380-2. [DOI] [PubMed] [Google Scholar]
  2. Ashby P., Bennett D. P., Spencer I. M., Robinson D. S. Post-translational regulation of lipoprotein lipase activity in adipose tissue. Biochem J. 1978 Dec 15;176(3):865–872. doi: 10.1042/bj1760865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bagby G. J., Liu M. S., Spitzer J. A. Lipoprotein lipase activity in rat heart myocytes. Life Sci. 1977 Aug 1;21(3):467–473. doi: 10.1016/0024-3205(77)90529-x. [DOI] [PubMed] [Google Scholar]
  4. Berry M. N., Friend D. S., Scheuer J. Morphology and metabolism of intact muscle cells isolated from adult rat heart. Circ Res. 1970 Jun;26(6):679–687. doi: 10.1161/01.res.26.6.679. [DOI] [PubMed] [Google Scholar]
  5. Borensztajn J., Robinson D. S. The effect of fasting on the utilization of chylomicron triglyceride fatty acids in relation to clearing factor lipase (lipoprotein lipase) releasable by heparin in the perfused rat heart. J Lipid Res. 1970 Mar;11(2):111–117. [PubMed] [Google Scholar]
  6. Borensztajn J., Rone M. S., Sandros T. Effects of colchicine and cycloheximide on the functional and non-functional lipoprotein lipase fractions of rat heart. Biochim Biophys Acta. 1975 Sep 19;398(3):394–400. doi: 10.1016/0005-2760(75)90190-3. [DOI] [PubMed] [Google Scholar]
  7. Carlson E. C., Grosso D. S., Romero S. A., Frangakis C. J., Byus C. V., Bressler R. Ultrastructural studies of metabolically active isolated adult rat heart myocytes. J Mol Cell Cardiol. 1978 May;10(5):449–459. doi: 10.1016/0022-2828(78)90366-8. [DOI] [PubMed] [Google Scholar]
  8. Chajek T., Stein O., Stein Y. Interference with the transport of heparin-releasable lipoprotein lipase in the perfused rat heart by colchicine and vinblastine. Biochim Biophys Acta. 1975 May 22;388(2):260–268. doi: 10.1016/0005-2760(75)90131-9. [DOI] [PubMed] [Google Scholar]
  9. Chajek T., Stein O., Stein Y. Lipoprotein lipase of cultured mesenchymal rat heart cells. I. Synthesis, secretion and releasability by heparin. Biochim Biophys Acta. 1978 Mar 30;528(3):456–465. doi: 10.1016/0005-2760(78)90035-8. [DOI] [PubMed] [Google Scholar]
  10. Chajek T., Stein O., Stein Y. Lipoprotein lipase of cultured mesenchymal rat heart cells. II. Hydrolysis of labeled vary low density lipoprotein triacylglycerol by membrane-supported enzyme. Biochim Biophys Acta. 1978 Mar 30;528(3):466–474. doi: 10.1016/0005-2760(78)90036-x. [DOI] [PubMed] [Google Scholar]
  11. Chajek T., Stein O., Stein Y. Rat heart in culture as a tool to elucidate the cellular origin of lipoprotein lipase. Biochim Biophys Acta. 1977 Jul 20;488(1):140–144. doi: 10.1016/0005-2760(77)90131-x. [DOI] [PubMed] [Google Scholar]
  12. Chohan P., Cryer A. Lipoprotein lipase activity of rat cardiac muscle. The intracellular distribution of the enzyme between fractions prepared from cardiac muscle and cells isolated from the hearts of fed and starved animals. Biochem J. 1979 Jul 1;181(1):83–93. doi: 10.1042/bj1810083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Chohan P., Cryer A. Stability of lipoprotein lipase (clearing-factor lipase) in rat cardiac muscle [proceedings]. Biochem Soc Trans. 1977;5(5):1340–1343. doi: 10.1042/bst0051340. [DOI] [PubMed] [Google Scholar]
  14. Chohan P., Cryer A. The lipoprotein lipase (clearing-factor lipase) activity of cells isolated from rat cardiac muscle. Biochem J. 1978 Aug 15;174(2):663–666. doi: 10.1042/bj1740663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Cryer A., Davies P., Williams E. R., Robinson D. S. The clearing-factor lipase activity of isolated fat-cells. Biochem J. 1975 Feb;146(2):481–488. doi: 10.1042/bj1460481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Cryer A., Foster B., Wing D. R., Robinson D. S. The effect of cycloheximide on adipose-tissue clearing-factor lipase. Biochem J. 1973 Apr;132(4):833–836. doi: 10.1042/bj1320833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Cryer A., Jones H. M. Changes in the lipoprotein lipase (clearing-factor lipase) activity of white adipose tissue during development of the rat. Biochem J. 1978 May 15;172(2):319–325. doi: 10.1042/bj1720319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Dani A. M., Cittadini A., Flamini G., Festuccia G., Terranova T. Preparation and some properties of isolated beating myocytes from adult rabbit heart. J Mol Cell Cardiol. 1977 Sep;9(9):777–784. doi: 10.1016/s0022-2828(77)80022-9. [DOI] [PubMed] [Google Scholar]
  19. East A. G., Louis L. N., Hoffenberg R. Albumin synthesis by isolated rat liver cells. Exp Cell Res. 1973 Jan;76(1):41–46. doi: 10.1016/0014-4827(73)90416-3. [DOI] [PubMed] [Google Scholar]
  20. Farmer B. B., Harris R. A., Jolly W. W., Hathaway D. R., Katzberg A., Watanabe A. M., Whitlow A. L., Besch H. R., Jr Isolation and characterization of adult rat hearts cells. Arch Biochem Biophys. 1977 Mar;179(2):545–558. doi: 10.1016/0003-9861(77)90143-6. [DOI] [PubMed] [Google Scholar]
  21. Fielding C. J., Higgins J. M. Lipoprotein lipase: comparative properties of the membrane-supported and solubilized enzyme species. Biochemistry. 1974 Oct 8;13(21):4324–4330. doi: 10.1021/bi00718a013. [DOI] [PubMed] [Google Scholar]
  22. Grove D., Zak R., Nair K. G., Aschenbrenner V. Biochemical correlates of cardiac hypertrophy. IV. Observations on the cellular organization of growth during myocardial hypertrophy in the rat. Circ Res. 1969 Oct;25(4):473–485. doi: 10.1161/01.res.25.4.473. [DOI] [PubMed] [Google Scholar]
  23. Howard R. B., Pesch L. A. Respiratory activity of intact, isolated parenchymal cells from rat liver. J Biol Chem. 1968 Jun 10;243(11):3105–3109. [PubMed] [Google Scholar]
  24. Klinge O., Stöcker E. Die DNS-Synthese im Rattenherzen als Funktion des Lebensalters. Autoradiographische Untersuchungen mit 3H-Thymidin. Experientia. 1968 Feb 15;24(2):167–168. doi: 10.1007/BF02146965. [DOI] [PubMed] [Google Scholar]
  25. Kornhauser D. M., Vaughan M. Release of lipoprotein lipase from fat cells in vitro. Biochim Biophys Acta. 1975 Jan 24;380(1):97–105. doi: 10.1016/0005-2760(75)90048-x. [DOI] [PubMed] [Google Scholar]
  26. Liu M. S., Spitzer J. J. Oxidation of palmitate and lactate by beating myocytes isolated from adult dog heart. J Mol Cell Cardiol. 1978 May;10(5):415–426. doi: 10.1016/0022-2828(78)90363-2. [DOI] [PubMed] [Google Scholar]
  27. Miller L. V., Beigelman P. M. Effects of varying concentrations of insulin upon protein synthesis in isolated fat cells. Endocrinology. 1967 Aug;81(2):386–389. doi: 10.1210/endo-81-2-386. [DOI] [PubMed] [Google Scholar]
  28. Morkin E., Ashford T. P. Myocardial DNA synthesis in experimental cardiac hypertrophy. Am J Physiol. 1968 Dec;215(6):1409–1413. doi: 10.1152/ajplegacy.1968.215.6.1409. [DOI] [PubMed] [Google Scholar]
  29. Olivecrona T., Bengtsson G., Marklund S. E., Lindahl U., Hök M. Heparin-lipoprotein lipase interactions. Fed Proc. 1977 Jan;36(1):60–65. [PubMed] [Google Scholar]
  30. Powell T., Twist V. W. A rapid technique for the isolation and purification of adult cardiac muscle cells having respiratory control and a tolerance to calcium. Biochem Biophys Res Commun. 1976 Sep 7;72(1):327–333. doi: 10.1016/0006-291x(76)90997-9. [DOI] [PubMed] [Google Scholar]
  31. Robinson D. S., Wing D. R. Studies on tissue clearing factor lipase related to its role in the removal of lipoprotein triglyceride from the plasma. Biochem Soc Symp. 1971;(33):123–135. [PubMed] [Google Scholar]
  32. Rogers M. P., Robinson D. S. Effects of cold exposure on heart clearing factor lipase and triglyceride utilization in the rat. J Lipid Res. 1974 May;15(3):263–272. [PubMed] [Google Scholar]
  33. Salaman M. R., Robinson D. S. Clearing-factor lipase in adipose tissue. A medium in which the enzyme activity of tissue from starved rats increases in vitro. Biochem J. 1966 Jun;99(3):640–647. doi: 10.1042/bj0990640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Spencer I. M., Hutchinson A., Robinson D. S. The effect of nutritional state on the lipoprotein lipase activity of isolated fat cells. Biochim Biophys Acta. 1978 Sep 28;530(3):375–384. doi: 10.1016/0005-2760(78)90157-1. [DOI] [PubMed] [Google Scholar]
  35. Stewart J. E., Schotz M. C. Release of lipoprotein lipase activity from isolated fat cells. II. Effect of heparin. J Biol Chem. 1974 Feb 10;249(3):904–907. [PubMed] [Google Scholar]
  36. Stewart J. E., Schotz M. C. Stimulation of release of lipoprotein lipase activity from isolated fat cells by rat serum. Nat New Biol. 1973 Aug 22;244(138):250–251. doi: 10.1038/newbio244250a0. [DOI] [PubMed] [Google Scholar]
  37. Stewart J. E., Schotz M. C. Studies on release of lipoprotein lipase activity from fat cells. J Biol Chem. 1971 Sep 25;246(18):5749–5753. [PubMed] [Google Scholar]
  38. WARBURG O., KRIPPAHL G. [Further development of manometric methods]. Z Naturforsch B. 1959 Aug-Sep;14B:561–563. [PubMed] [Google Scholar]
  39. Wing D. R., Robinson D. S. Clearing-factor lipase in adipose tissue. Studies with puromycin and actinomycin. Biochem J. 1968 Feb;106(3):667–676. doi: 10.1042/bj1060667. [DOI] [PMC free article] [PubMed] [Google Scholar]

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