Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1979 Nov 15;184(2):431–440. doi: 10.1042/bj1840431

Androgen-sensitive spermine-binding protein of rat ventral prostate. Purification of the protein and characterization of the hormonal effect.

G Mezzetti, R Loor, S Liao
PMCID: PMC1161779  PMID: 534539

Abstract

The rat ventral prostate contains a cytosol protein that can non-covalently bind spermine much more tightly than spermidine or other natural diamines. The protein has been purified to homogeneity, as judged by electrophoresis in urea- and sodium dodecyl sulphate-containing polyacrylamide gels. The protein, with or without spermine bound to it, sediments at 3 S in a sucrose gradient with or without 0.4 M-KCl. The molecular weight of the protein is about 30 000. Each molecule of the binding protein can bind one molecule of spermine. In the prostate of rats injected with cycloheximide, the protein appears to have a half-life of about 3.5 h. The spermine-binding activity of an acidic fraction obtained by DEAE-cellulose chromatography of the prostate cytosol proteins is reduced by about 40--60% within 20--40 h after castration. This effect is reversed very rapidly within 15--30 min by intraperitoneal injection of 5 alpha-dihydrotestosterone. The hormonal effect is androgen-specific and is not mimicked by dexamethasone or oestradiol-17 beta. The androgen effect was reduced significantly when rats were injected with cycloheximide or actinomycin D, suggesting that the acidic protein may be one of the earliest proteins induced by androgen in the rat ventral prostate.

Full text

PDF
440

Images in this article

433Fig. 1.
on p.433

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdel-Monem M., Ohno K. Separation of the Dns derivatives of polyamines and related compounds by thin-layer and high-pressure liquid chromatography. J Chromatogr. 1975 Apr 16;107(2):416–419. doi: 10.1016/0021-9673(75)80022-7. [DOI] [PubMed] [Google Scholar]
  2. Ahmed K., Ishida H. Effect of testosterone on nuclear phosphoproteins of rat ventral prostate. Mol Pharmacol. 1971 May;7(3):323–327. [PubMed] [Google Scholar]
  3. Ahmed K., Wilson M. J., Goueli S. A., Williams-Ashman H. G. Effects of polyamines on prostatic chromatin- and non-histone-protein-associated protein kinase reactions. Biochem J. 1978 Dec 15;176(3):739–750. doi: 10.1042/bj1760739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barnea A., Gorski J. Estrogen-induced protein. Time course of synthesis. Biochemistry. 1970 Apr 28;9(9):1899–1904. doi: 10.1021/bi00811a006. [DOI] [PubMed] [Google Scholar]
  5. Chen C., Hiipakka R. A., Liao S. Prostate alpha-protein: subunit structure, polyamine binding, and inhibition of nuclear chromatin binding of androgen-receptor complex. J Steroid Biochem. 1979 Jul;11(1B):401–405. doi: 10.1016/0022-4731(79)90058-x. [DOI] [PubMed] [Google Scholar]
  6. Couch R. M., Anderson K. M. Rat ventral prostate chromatin. Effect of androgens on its chemical composition, physical properties, and template activity. Biochemistry. 1973 Jul 31;12(16):3114–3121. doi: 10.1021/bi00740a027. [DOI] [PubMed] [Google Scholar]
  7. DeAngelo A. B., Gorski J. Role of RNA synthesis in the estrogen induction of a specific uterine protein. Proc Natl Acad Sci U S A. 1970 Jul;66(3):693–700. doi: 10.1073/pnas.66.3.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  9. Fang S., Liao S. Androgen receptors. Steroid- and tissue-specific retention of a 17 beta-hydroxy-5 alpha-androstan-3-one-protein complex by the cell nuclei of ventral prostate. J Biol Chem. 1971 Jan 10;246(1):16–24. [PubMed] [Google Scholar]
  10. Heyns W., Peeters B., Mous J., Rombauts W., De Moor P. Purification and characterisation of prostatic binding protein and its subunits. Eur J Biochem. 1978 Aug 15;89(1):181–186. doi: 10.1111/j.1432-1033.1978.tb20910.x. [DOI] [PubMed] [Google Scholar]
  11. Kostraba N. C., Montagna R. A., Wang T. Y. Study of the loosely bound non-histone chromatin proteins. Stimulation of deoxyribonucleic acid-templated ribonucleic acid synthesis by a specific deoxyribonucleic acid-binding phosphoprotein fraction. J Biol Chem. 1975 Feb 25;250(4):1548–1555. [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Liang T., Castañeda E., Liao S. Androgen and initiation of protein synthesis in the prostate. Binding of Met-tRNAfMet to cytosol initiation factor and ribosomal subunit particles. J Biol Chem. 1977 Aug 25;252(16):5692–5700. [PubMed] [Google Scholar]
  15. Liang T., Mezzetti G., Chen C., Liao S. Selective polyamine-binding proteins. Spermine binding by an androgen-sensitive phosphoprotein. Biochim Biophys Acta. 1978 Sep 6;542(3):430–441. doi: 10.1016/0304-4165(78)90374-4. [DOI] [PubMed] [Google Scholar]
  16. Liu A. Y., Greengard P. Regulation by steroid hormones of phosphorylation of specific protein common to several target organs. Proc Natl Acad Sci U S A. 1976 Feb;73(2):568–572. doi: 10.1073/pnas.73.2.568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Loor R. M., Hu A. L., Wang T. Y. Structurally altered and transcriptionally activated rat prostate chromatin induced by androgens. Biochim Biophys Acta. 1977 Aug 2;477(3):312–321. doi: 10.1016/0005-2787(77)90055-7. [DOI] [PubMed] [Google Scholar]
  18. Mainwaring W. I., Jones D. M. Influence of receptor complexes on the properties of prostate chromatin, including its transcription by RNA polymerase. J Steroid Biochem. 1975 Mar-Apr;6(3-4):475–481. doi: 10.1016/0022-4731(75)90175-2. [DOI] [PubMed] [Google Scholar]
  19. Tabor C. W., Tabor H. 1,4-Diaminobutane (putrescine), spermidine, and spermine. Annu Rev Biochem. 1976;45:285–306. doi: 10.1146/annurev.bi.45.070176.001441. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES