Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1980 May 1;187(2):521–524. doi: 10.1042/bj1870521

Heparin enhances the rate of binding of fibronectin to collagen.

S Johansson, M Höök
PMCID: PMC1161819  PMID: 6156674

Abstract

125I-labelled fibronectin is shown to bind to both native and denatured collagen immobilized on Sephadex beads in reactions that exhibit different kinetics. The rates of both reactions were enhanced by the presence of heparin or highly sulphated dextran sulphate but not by other glycosaminoglycans or dextran sulphates having low sulphate contents.

Full text

PDF
521

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bäckström G., Hök M., Lindahl U., Feingold D. S., Malmström A., Rodén L., Jacobsson I. Biosynthesis of heparin. Assay and properties of the microsomal uronosyl C-5 epimerase. J Biol Chem. 1979 Apr 25;254(8):2975–2982. [PubMed] [Google Scholar]
  2. Dessau W., Adelmann B. C., Timpl R. Identification of the sites in collagen alpha-chains that bind serum anti-gelatin factor (cold-insoluble globulin). Biochem J. 1978 Jan 1;169(1):55–59. doi: 10.1042/bj1690055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Engvall E., Ruoslahti E., Miller E. J. Affinity of fibronectin to collagens of different genetic types and to fibrinogen. J Exp Med. 1978 Jun 1;147(6):1584–1595. doi: 10.1084/jem.147.6.1584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hedman K., Kurkinen M., Alitalo K., Vaheri A., Johansson S., Hök M. Isolation of the pericellular matrix of human fibroblast cultures. J Cell Biol. 1979 Apr;81(1):83–91. doi: 10.1083/jcb.81.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hopper K. E., Adelmann B. C., Gentner G., Gay S. Recongnition by guinea-pig peritoneal exudate cells of conformationally different states of the collagen molecule. Immunology. 1976 Feb;30(2):249–259. [PMC free article] [PubMed] [Google Scholar]
  6. Hök M., Björk I., Hopwood J., Lindahl U. Anticoagulant activity of heparin: separation of high-activity and low-activity heparin species by affinity chromatography on immobilized antithrombin. FEBS Lett. 1976 Jul 1;66(1):90–93. doi: 10.1016/0014-5793(76)80592-3. [DOI] [PubMed] [Google Scholar]
  7. Jilek F., Hörmann H. Cold-insoluble globulin (fibronectin), IV[1-35 affinity to soluble collagen of various types. Hoppe Seylers Z Physiol Chem. 1978 Feb;359(2):247–250. doi: 10.1515/bchm.1978.359.1.247. [DOI] [PubMed] [Google Scholar]
  8. Jilek F., Hörmann H. Fibronectin (cold-insoluble globulin), VI. Influence of heparin and hyaluronic acid on the binding of native collagen. Hoppe Seylers Z Physiol Chem. 1979 Apr;360(4):597–603. doi: 10.1515/bchm2.1979.360.1.597. [DOI] [PubMed] [Google Scholar]
  9. Johansson S., Rubin K., Hök M., Ahlgren T., Seljelid R. In vitro biosynthesis of cold insoluble globulin (fibronectin) by mouse peritoneal macrophages. FEBS Lett. 1979 Sep 15;105(2):313–316. doi: 10.1016/0014-5793(79)80637-7. [DOI] [PubMed] [Google Scholar]
  10. Klebe R. J. Isolation of a collagen-dependent cell attachment factor. Nature. 1974 Jul 19;250(463):248–251. doi: 10.1038/250248a0. [DOI] [PubMed] [Google Scholar]
  11. Obrink B., Laurent T. C., Carlsson B. The binding of chondroitin sulphate to collagen. FEBS Lett. 1975 Aug 1;56(1):166–169. doi: 10.1016/0014-5793(75)80133-5. [DOI] [PubMed] [Google Scholar]
  12. Obrink B. Non-aggregated tropocollagen at physiological pH and ionic strength. A chemical and physico-chemical characterization of tropocollagen isolated from the skin of lathyritic rats. Eur J Biochem. 1972 Feb;25(3):563–572. doi: 10.1111/j.1432-1033.1972.tb01729.x. [DOI] [PubMed] [Google Scholar]
  13. Pearlstein E. Plasma membrane glycoprotein which mediates adhesion of fibroblasts to collagen. Nature. 1976 Aug 5;262(5568):497–500. doi: 10.1038/262497a0. [DOI] [PubMed] [Google Scholar]
  14. Perkins M. E., Ji T. H., Hynes R. O. Cross-linking of fibronectin to sulfated proteoglycans at the cell surface. Cell. 1979 Apr;16(4):941–952. doi: 10.1016/0092-8674(79)90109-0. [DOI] [PubMed] [Google Scholar]
  15. Rubin K., Oldberg A., Hök M., Obrink B. Adhesion of rat hepatocytes to collagen. Exp Cell Res. 1978 Nov;117(1):165–177. doi: 10.1016/0014-4827(78)90439-1. [DOI] [PubMed] [Google Scholar]
  16. Saba T. M., Blumenstock F. A., Weber P., Kaplan J. E. Physiologic role of cold-insoluble globulin in systemic host defense: implications of its characterization as the opsonic alpha 2-surface-binding glycoprotein. Ann N Y Acad Sci. 1978 Jun 20;312:43–55. doi: 10.1111/j.1749-6632.1978.tb16792.x. [DOI] [PubMed] [Google Scholar]
  17. Yamada K. M., Olden K. Fibronectins--adhesive glycoproteins of cell surface and blood. Nature. 1978 Sep 21;275(5677):179–184. doi: 10.1038/275179a0. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES