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. 1979 Dec 15;184(3):565–569. doi: 10.1042/bj1840565

Acetate is the preferred substrate for long-chain fatty acid synthesis in isolated spinach chloroplasts.

P G Roughan, R Holland, C R Slack
PMCID: PMC1161838  PMID: 540048

Abstract

1. Commercially available [2-14C]pyruvate and [2-14C]malonate were found to contain 3-6% (w/w) of [14C]acetate. 2. The contaminating [14C]acetate was efficiently utilized for fatty acid synthesis by isolated chloroplasts, whereas the parent materials were poorer substrates. 3. Maximum incorporation rates of the different substrates examined were (ng-atoms of C/h per mg of chlorophyll): [1-14C]acetate, 2676; [2-14C]pyruvate, 810; H14CO3-, 355; [2-14C]malonate, 19. 4. Products of CO2 fixation were probably not a significant carbon source for fatty acid synthesis in the presence of exogenous acetate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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