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. 1980 Jul 1;189(1):27–34. doi: 10.1042/bj1890027

Studies on the mechanism of binding of serum albumins to immobilized cibacron blue F3G A.

R J Leatherbarrow, P D Dean
PMCID: PMC1161914  PMID: 7458904

Abstract

The interaction of Cibacron Blue F3G A-Sepharose 4B with several serum albumins was studied. Although all albumins used were fond to bind to this adsorbent, human serum albumin was bound to a far greater extent than were the others. From the results of competition experiments and n.m.r. studies of Cibacron Blue and/or bilirubin binding to human serum albumin it is proposed that the mechanism of the interaction between human serum albumin and cibacron Blue is consistent wit Cibacron Blue binding to bilirubin-binding sites. In contrast with these findings with human serum albumin, there is little or no interaction of Cibacron Blue and the bilirubin-binding sites of albumins from rabbit, horse, bovine or sheep sera, although some interaction occurs between Cibacron Blue and the fatty acid-binding sites of these proteins. Structural analogues of Cibacron Blue have been used to investigate the binding of albumins to these ligands.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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