Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1980 Dec 1;191(3):855–858. doi: 10.1042/bj1910855

Immobilization of DNA for affinity chromatography and drug-binding studies.

A J Macdougall, J R Brown, T W Plumbridge
PMCID: PMC1162286  PMID: 7283977

Abstract

A method is described in which double-stranded DNA is alkylated with 4-bis-(2-chloroethyl)amino-L-phenylalanine and the product immobilized on an insoluble support via the primary amino group of the phenylalanine moiety. The DNA is hence irreversibly bound to the matrix by both strands at a limited number of points.

Full text

PDF
856

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberts B. M., Amodio F. J., Jenkins M., Gutmann E. D., Ferris F. L. Studies with DNA-cellulose chromatography. I. DNA-binding proteins from Escherichia coli. Cold Spring Harb Symp Quant Biol. 1968;33:289–305. doi: 10.1101/sqb.1968.033.01.033. [DOI] [PubMed] [Google Scholar]
  2. Arndt-Jovin D. J., Jovin T. M., Bähr W., Frischauf A. M., Marquardt M. Covalent attachment of DNA to agarose. Improved synthesis and use in affinity chromatography. Eur J Biochem. 1975 Jun;54(2):411–418. doi: 10.1111/j.1432-1033.1975.tb04151.x. [DOI] [PubMed] [Google Scholar]
  3. BAUTZ E. K., HALL B. D. The isolation of T4-specific RNA on a DNA-cellulose column. Proc Natl Acad Sci U S A. 1962 Mar 15;48:400–408. doi: 10.1073/pnas.48.3.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bagioni S., Sisto R., Ferraro A., Caiafa P., Turano C. A new method for the preparation of DNA--cellulose. Anal Biochem. 1978 Sep;89(2):616–619. doi: 10.1016/0003-2697(78)90390-1. [DOI] [PubMed] [Google Scholar]
  5. Cavalieri L. F., Carroll E. A DNA-acrylamide gel column for analyzing proteins that bind to DNA. I. DNA polymerase. Proc Natl Acad Sci U S A. 1970 Oct;67(2):807–812. doi: 10.1073/pnas.67.2.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dickerman H. W., Ryan T. J., Bass A. I., Chatterjee N. K. Preparation of azoDNA and its use in affinity chromatography. Arch Biochem Biophys. 1978 Mar;186(2):218–234. doi: 10.1016/0003-9861(78)90431-9. [DOI] [PubMed] [Google Scholar]
  7. Inagaki A., Kageyama M. Interaction of antibiotics with deoxyribonucleic acid. II. DNA-cellulose chromatography of antibiotics and related compounds. J Biochem. 1970 Aug;68(2):187–192. doi: 10.1093/oxfordjournals.jbchem.a129345. [DOI] [PubMed] [Google Scholar]
  8. Jovin T. M., Kornberg A. Polynucleotide celluloses as solid state primers and templates for polymerases. J Biol Chem. 1968 Jan 25;243(2):250–259. [PubMed] [Google Scholar]
  9. Litman R. M. A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose. J Biol Chem. 1968 Dec 10;243(23):6222–6233. [PubMed] [Google Scholar]
  10. Plumbridge T. W., Brown J. R. Spectrophotometric and fluorescence polarization studies of the binding of ethidium, daunomycin and mepacrine to DNA and to poly(I-C). Biochim Biophys Acta. 1977 Dec 14;479(4):441–449. doi: 10.1016/0005-2787(77)90037-5. [DOI] [PubMed] [Google Scholar]
  11. Poonian M. S., Schlabach A. J., Weissbach A. Covalent attachment of nucleic acids to agarose for affinity chromatography. Biochemistry. 1971 Feb 2;10(3):424–427. doi: 10.1021/bi00779a011. [DOI] [PubMed] [Google Scholar]
  12. Potuzak H., Dean P. D. Affinity adsorbents consisting of nucleic acids immobilized via bisoxirane activated polysaccharides. Nucleic Acids Res. 1978 Jan;5(1):297–303. doi: 10.1093/nar/5.1.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Potuzak H., Wintersberger U. DNA covalently linked to carboxymethyl-cellulose and its application in affinity chromatography. FEBS Lett. 1976 Mar 15;63(1):167–170. doi: 10.1016/0014-5793(76)80218-9. [DOI] [PubMed] [Google Scholar]
  14. Rickwood D. An improved method for the insolubilization of DNA for affinity chromatography. Biochim Biophys Acta. 1972 Apr 26;269(1):47–50. doi: 10.1016/0005-2787(72)90072-x. [DOI] [PubMed] [Google Scholar]
  15. Schaller H., Nüsslein C., Bonhoeffer F. J., Kurz C., Nietzschmann I. Affinity chromatography of DNA-binding enzymes on single-stranded DNA-agarose columns. Eur J Biochem. 1972 Apr 24;26(4):474–481. doi: 10.1111/j.1432-1033.1972.tb01789.x. [DOI] [PubMed] [Google Scholar]
  16. Weissbach A., Poonian M. Nucleic acids attached to solid matrices. Methods Enzymol. 1974;34:463–475. doi: 10.1016/s0076-6879(74)34057-8. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES