Abstract
Leupeptin, antipain, tosyl-lysylchloromethane (Tos-Lys-CH2Cl) and benzyloxy-carbonylphenylalanylalanyldiazomethane (Z-Phe-Ala-CHN2) inhibit reversibly the resorption induced by parathyroid hormone or heparin in cultured mouse bones. Leupeptin and antipain do not affect collagenase production and activity or the enhanced secretion of beta-glucuronidase induced by the bone-resorbing agents. They might thus act by a direct (extracellular?) inhibition of lysosomal thiol proteinases.
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Selected References
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- Burleigh M. C., Barrett A. J., Lazarus G. S. Cathepsin B1. A lysosomal enzyme that degrades native collagen. Biochem J. 1974 Feb;137(2):387–398. doi: 10.1042/bj1370387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ducastaing A., Etherington D. J. Purification of bovine spleen collagenolytic cathepsin, (cathepsin N). Biochem Soc Trans. 1978;6(5):938–940. doi: 10.1042/bst0060938. [DOI] [PubMed] [Google Scholar]
- Lenaers-Claeys G., Vaes G. Collagenase, procollagenase and bone resorption. Effects of heparin, parathyroid hormone and calcitonin. Biochim Biophys Acta. 1979 May 16;584(3):375–388. doi: 10.1016/0304-4165(79)90114-4. [DOI] [PubMed] [Google Scholar]
- Robin R., Chou I. N., Black P. H. Proteolytic enzymes, cell surface changes, and viral transformation. Adv Cancer Res. 1975;22:203–260. doi: 10.1016/s0065-230x(08)60178-5. [DOI] [PubMed] [Google Scholar]
- Robins E., Hirsch H. E., Emmons S. S. Glycosidases in the nervous system. I. Assay, some properties, and distribution of beta-galactosidase, beta-glucoronidase, and beta-glucosidase. J Biol Chem. 1968 Aug 25;243(16):4246–4252. [PubMed] [Google Scholar]
- Shaw E., Dean R. T. The inhibition of macrophage protein turnover by a selective inhibitor of thiol proteinases. Biochem J. 1980 Feb 15;186(2):385–390. doi: 10.1042/bj1860385. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vaes G. Excretion of acid and of lysosomal hydrolytic enzymes during bone resorption induced in tissue culture by parathyroid extract. Exp Cell Res. 1965 Sep;39(2):470–474. doi: 10.1016/0014-4827(65)90050-9. [DOI] [PubMed] [Google Scholar]
- Vaes G. On the mechanisms of bone resorption. The action of parathyroid hormone on the excretion and synthesis of lysosomal enzymes and on the extracellular release of acid by bone cells. J Cell Biol. 1968 Dec;39(3):676–697. doi: 10.1083/jcb.39.3.676. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vaes G. The release of collagenase as an inactive proenzyme by bone explants in culture. Biochem J. 1972 Jan;126(2):275–289. doi: 10.1042/bj1260275. [DOI] [PMC free article] [PubMed] [Google Scholar]