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. 1980 Jun 1;187(3):851–856. doi: 10.1042/bj1870851

The serum competitor of oestrogen--rat alpha 1-foetoprotein interactions. Identification as a mixture of non-esterified fatty acids.

G Vallette, C Benassayag, L Savu, J Delorme, E A Nunez, J Doumas, G Maume, B F Maume
PMCID: PMC1162471  PMID: 6204639

Abstract

The novel endogenous serum ligands of rat alpha 1-foetoprotein previously demonstrated in different mammalian sera were identified by g.l.c.--mass-spectrometric methods as a mixture of non-esterified long-chain and predominantly unsaturated fatty acids. Detailed comparative analyses of these ligands extracted from foetal- and pregnant-rat sera, rat amniotic fluid and foetal human sera are presented. We also show that an important fraction of these ligands remains associated with the rat alpha 1-foetoprotein after purification; analyses are given for the composition of this lipid moiety of the foetoprotein. The physiological relevance of these results is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benassayag C., Savu L., Vallette G., Delorme J., Nunez E. A. Relations between fatty acids and oestrogen binding properties of pure rat alpha 1-foetoprotein. Biochim Biophys Acta. 1979 Oct 4;587(2):227–237. doi: 10.1016/0304-4165(79)90356-8. [DOI] [PubMed] [Google Scholar]
  2. Benassayag C., Vallette G., Cittanova N., Nunez E., Jayle M. F. Isolation of two forms of rat alpha-fetoprotein and comparison of their binding parameters with estradiol-17beta. Biochim Biophys Acta. 1975 Dec 15;412(2):295–305. doi: 10.1016/0005-2795(75)90044-6. [DOI] [PubMed] [Google Scholar]
  3. Benassayag C., Vallette G., Delorme J., Savu L., Nunez E. A., Jayle M. F. Rat and human embryo and post-natal sera contain a potent endogenous competitor of estrogen-rat alpha-fetoprotein interactions. Steroids. 1977 Dec;30(6):771–785. doi: 10.1016/s0039-128x(77)80023-8. [DOI] [PubMed] [Google Scholar]
  4. Crawford M. A., Hassam A. G., Williams G. Essential fatty acids and fetal brain growth. Lancet. 1976 Feb 28;1(7957):452–453. doi: 10.1016/s0140-6736(76)91476-8. [DOI] [PubMed] [Google Scholar]
  5. Crawford M. A., Hassam A. G., Williams G., Whitehouse W. Fetal accumulation of long-chain polyunsaturated fatty acids. Adv Exp Med Biol. 1977;83:135–143. doi: 10.1007/978-1-4684-3276-3_13. [DOI] [PubMed] [Google Scholar]
  6. DeWille J. W., Fraker P. J., Romsos D. R. Effects of essential fatty acid deficiency, and various levels of dietary polyunsaturated fatty acids, on humoral immunity in mice. J Nutr. 1979 Jun;109(6):1018–1027. doi: 10.1093/jn/109.6.1018. [DOI] [PubMed] [Google Scholar]
  7. FRIEDMAN M., BYERS S. O. Effects of diet on serum lipids of fetal, neonatal, and pregnant rabbits. Am J Physiol. 1961 Oct;201:611–616. doi: 10.1152/ajplegacy.1961.201.4.611. [DOI] [PubMed] [Google Scholar]
  8. Gammal E. B., Carroll K. K., Plunkett E. R. Effects of dietary fat on mammary carcinogenesis by 7,12-dimethylbenz(alpha)anthracene in rats. Cancer Res. 1967 Oct;27(10):1737–1742. [PubMed] [Google Scholar]
  9. HANSEN A. E., WIESE H. F., ADAM D. J., BOELSCHE A. N., HAGGARD M. E., DAVIS H., NEWSOM W. T., PESUT L. INFLUENCE OF DIET ON BLOOD SERUM LIPIDS IN PREGNANT WOMEN AND NEWBORN INFANTS. Am J Clin Nutr. 1964 Jul;15:11–19. doi: 10.1093/ajcn/15.1.11. [DOI] [PubMed] [Google Scholar]
  10. Hopkins G. J., Hard G. C., West C. E. Carcinogenesis induced by 7,12-dimethylbenz[a]anthracene in c3H-A vyfB mice: influence of different dietary fats. J Natl Cancer Inst. 1978 Apr;60(4):849–853. doi: 10.1093/jnci/60.4.849. [DOI] [PubMed] [Google Scholar]
  11. Nunez E., Benassayag C., Engelmann F., Vallette G., Hurst L., Jayle M. F. Binding of estrogens to serum protein of rats treated with hepatocarcinogenic compounds. Biomedicine. 1973 Nov;18(6):514–520. [PubMed] [Google Scholar]
  12. Nunez E., Engelmann F., Benassayag C., Jayle M. F. Identification et purification préliminaire de la faeto-protéine liant les aestrogènes dans le sérum de rats nouveau-nés. C R Acad Sci Hebd Seances Acad Sci D. 1971 Aug 30;273(9):831–834. [PubMed] [Google Scholar]
  13. Nunez E., Vallette G., Benassayag C., Jayle M. F. Comparative study on the binding of estrogens by human and rat serum proteins in development. Biochem Biophys Res Commun. 1974 Mar 15;57(1):126–133. doi: 10.1016/s0006-291x(74)80366-9. [DOI] [PubMed] [Google Scholar]
  14. Parmelee D. C., Evenson M. A., Deutsch H. F. The presence of fatty acids in human alpha-fetoprotein. J Biol Chem. 1978 Apr 10;253(7):2114–2119. [PubMed] [Google Scholar]
  15. Savu L., Benassayag C., Vallette G., Nunez E., Jayle M. F. Purification and estrogen binding properties of mouse alpha1-fetoprotein and of two forms of the protein with different affinities for concanavalin-A. Biochimie. 1977;59(3):323–328. doi: 10.1016/s0300-9084(77)80149-1. [DOI] [PubMed] [Google Scholar]
  16. Savu L., Crepy O., Guerin M. A., Nunez E., Engelmann F., Benassayag C., Jayle M. F. Etude des constantes de liaison entre les oestrogenes et 1'alpha(1)-foetoproteine de rat. FEBS Lett. 1972 Apr 15;22(1):113–116. doi: 10.1016/0014-5793(72)80233-3. [DOI] [PubMed] [Google Scholar]
  17. Tonkin C. H., Brostoff J. Do fatty acids exert a specific effect on human lymphocyte transformation in vitro? Int Arch Allergy Appl Immunol. 1978;57(2):171–176. doi: 10.1159/000232099. [DOI] [PubMed] [Google Scholar]
  18. Uriel J., de Nechaud B., Dupiers M. Estrogen-binding properties of rat, mouse and man fetospecific serum proteins. Demonstration by immuno-autoradiographic methods. Biochem Biophys Res Commun. 1972 Feb 16;46(3):1175–1180. doi: 10.1016/s0006-291x(72)80098-6. [DOI] [PubMed] [Google Scholar]

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