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. 1981 Jan 1;193(1):371–374. doi: 10.1042/bj1930371

Double-label reductive methylation of tissue proteins for precision two-dimensional polyacrylamide-gel electrophoretic analysis.

J M Finger, K H Choo
PMCID: PMC1162610  PMID: 7305933

Abstract

Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.

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Selected References

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